公开(公告)号：US20160007597A1

公开(公告)日：2016-01-14

申请号：US14771229

申请日：2014-03-14

申请人： SUNTORY HOLDINGS LIMITED

发明人： Keisuke MATSUI

IPC分类号： A01N37/18 ,  C12N15/87

CPC分类号： A01N37/18 ,  A01H3/04 ,  C07K7/06 ,  C12N15/87

摘要： The object of the present invention is to provide techniques for causing trait modification in plants by administering a desired substance from the extracellular environment.The present invention provides a substance delivery method for introduction of a desired substance into plant cells by treatment of a whole plant with a treating agent for plant cell walls, which comprises a chelator and a surfactant.

摘要翻译： 本发明的目的是提供通过从细胞外环境施用所需物质来引起植物中的性状修饰的技术。 本发明提供了通过用植物细胞壁处理剂处理整株植物将所需物质引入植物细胞的物质递送方法，其包括螯合剂和表面活性剂。

公开(公告)号：US20170058269A1

公开(公告)日：2017-03-02

申请号：US15308117

申请日：2015-05-01

申请人： SUNTORY HOLDINGS LIMITED

发明人： Yoshikazu TANAKA ,  Naoko OKITSU ,  Keisuke MATSUI

IPC分类号： C12N9/10 ,  C12P17/06

CPC分类号： C12N9/1048 ,  A01H5/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/09 ,  C12P17/06 ,  C12P19/60 ,  C12P21/00 ,  C12Y204/00

摘要： Provided is a polynucleotide for encoding a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone, particularly flavone 4′-glucoside. A polynucleotide selected from the group consisting of: (a) polynucleotides comprising a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5; (b) polynucleotides that hybridize, under stringent conditions, with polynucleotides comprising a base sequence complementary to a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5, wherein the polynucleotides encode a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; (c) polynucleotides for encoding proteins comprising an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6; (d) polynucleotides for encoding proteins comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted, and/or added in an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6, the polynucleotides exhibiting activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; and the like.

摘要翻译： 本发明提供一种编码蛋白质的多核苷酸，其表现出将糖转移到黄酮，特别是黄酮4'-葡萄糖苷的第7位的羟基的活性。 选自以下的多核苷酸：（a）包含SEQ ID NO：1或SEQ ID NO：5的碱基序列的多核苷酸; （b）在严格条件下与包含与SEQ ID NO：1或SEQ ID NO：5的碱基序列互补的碱基序列的多核苷酸杂交的多核苷酸，其中所述多核苷酸编码显示转移糖的活性的蛋白质 到黄酮的第7位的羟基; （c）编码包含SEQ ID NO：2或SEQ ID NO：6的氨基酸序列的蛋白质的多核苷酸; （d）用于编码蛋白质的多核苷酸，其包含其中一个或多个氨基酸已被缺失，取代，插入和/或加入到SEQ ID NO：2或SEQ ID NO：6的氨基酸序列中的氨基酸序列 表现出将糖转移到黄酮第7位羟基的活性的多核苷酸; 等等。