公开(公告)号：US20200283782A1

公开(公告)日：2020-09-10

申请号：US16753110

申请日：2018-10-02

申请人： Suntory Holdings Limited

发明人： Noriko NAKAMURA ,  Naoko OKITSU ,  Yoshikazu TANAKA

IPC分类号： C12N15/82 ,  C12N15/52 ,  C12N9/10 ,  A01H5/00 ,  C12N9/88

摘要： Provided are a transformed plant having a blue-hued flower color or its inbred or outbred progenies, or their propagules, partial plant bodies, tissues or cells.Delphinidin type anthocyanin and flavone C-glycoside are made to be coexpressed in petals.

公开(公告)号：US20190032066A1

公开(公告)日：2019-01-31

申请号：US15740944

申请日：2016-06-30

申请人： SUNTORY HOLDINGS LIMITED

发明人： Naonobu NODA ,  Ryutaro AIDA ,  Satoshi HONGO ,  Sanae SATO ,  Yoshikazu TANAKA

IPC分类号： C12N15/82 ,  C12N9/10 ,  C12N9/02

摘要： Provided are transformed chrysanthemum plants having blue flower color, their self-fertilized progenies or cross-fertilized progenies thereof, a vegetative propagated plants thereof, and a part, a tissue or a cell of the plant body. Anthocyanin 3′,5′-O-glucosyltransferase gene (CtA3′5′GT) derived from Clitoria ternatea and flavonoid 3′,5′-hydroxylase gene derived from Campanula (CamF3′5′H) are coexpressed in chrysanthemum petals.

公开(公告)号：US20170058269A1

公开(公告)日：2017-03-02

申请号：US15308117

申请日：2015-05-01

申请人： SUNTORY HOLDINGS LIMITED

发明人： Yoshikazu TANAKA ,  Naoko OKITSU ,  Keisuke MATSUI

IPC分类号： C12N9/10 ,  C12P17/06

CPC分类号： C12N9/1048 ,  A01H5/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/09 ,  C12P17/06 ,  C12P19/60 ,  C12P21/00 ,  C12Y204/00

摘要： Provided is a polynucleotide for encoding a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone, particularly flavone 4′-glucoside. A polynucleotide selected from the group consisting of: (a) polynucleotides comprising a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5; (b) polynucleotides that hybridize, under stringent conditions, with polynucleotides comprising a base sequence complementary to a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5, wherein the polynucleotides encode a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; (c) polynucleotides for encoding proteins comprising an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6; (d) polynucleotides for encoding proteins comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted, and/or added in an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6, the polynucleotides exhibiting activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; and the like.

摘要翻译： 本发明提供一种编码蛋白质的多核苷酸，其表现出将糖转移到黄酮，特别是黄酮4'-葡萄糖苷的第7位的羟基的活性。 选自以下的多核苷酸：（a）包含SEQ ID NO：1或SEQ ID NO：5的碱基序列的多核苷酸; （b）在严格条件下与包含与SEQ ID NO：1或SEQ ID NO：5的碱基序列互补的碱基序列的多核苷酸杂交的多核苷酸，其中所述多核苷酸编码显示转移糖的活性的蛋白质 到黄酮的第7位的羟基; （c）编码包含SEQ ID NO：2或SEQ ID NO：6的氨基酸序列的蛋白质的多核苷酸; （d）用于编码蛋白质的多核苷酸，其包含其中一个或多个氨基酸已被缺失，取代，插入和/或加入到SEQ ID NO：2或SEQ ID NO：6的氨基酸序列中的氨基酸序列 表现出将糖转移到黄酮第7位羟基的活性的多核苷酸; 等等。

公开(公告)号：US20140165235A1

公开(公告)日：2014-06-12

申请号：US14156961

申请日：2014-01-16

申请人： SUNTORY HOLDINGS LIMITED

发明人： Yoshikazu TANAKA ,  Filippa Brugliera

IPC分类号： C12N15/82

CPC分类号： C12N15/825 ,  C12N9/0073 ,  C12N15/8225

摘要： Disclosed are: a novel cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H); and a novel F3′5′H gene construct which carries the polynucleotide. Specifically disclosed are: a cineraria-derived petal-specific promoter which is selected from the group consisting of (1) a polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 and (2) a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 under stringent conditions and can act as a promoter for a coding region of flavonoid 3′,5′-hydroxylase (F3′5′H); and a gene construct for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the novel cineraria-derived polynucleotide.

摘要翻译： 本发明涉及一种新型的来源于线粒体的多核苷酸，其可以作为黄酮3'，5'-羟化酶（F3'5'H）的编码区的启动子。 和携带多核苷酸的新型F3'5'H基因构建体。 具体公开的是：来自花粉衍生的花瓣特异性启动子，其选自（1）具有SEQ ID NO：9所示核苷酸序列的多核苷酸和（2）可与具有SEQ ID NO：9的多核苷酸杂交的多核苷酸， 在严格条件下显示于SEQ ID NO：9中的核苷酸序列，可作为黄酮3'，5'-羟化酶（F3'5'H）编码区的启动子。 以及用于以RNAi方法抑制基因表达的基因构建体，其具有作为环的多核苷酸，所述多核苷酸包含全新一代来源于多形核苷酸的多核苷酸的第一内含子的全部或一部分。