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    NOVEL GLYCOSYLTRANSFERASE GENE AND USE THEREOF
    1.
    发明申请
    NOVEL GLYCOSYLTRANSFERASE GENE AND USE THEREOF 审中-公开
    新型GLYCOSYLTRANSFERASE基因及其用途

    公开(公告)号：US20170058269A1

    公开(公告)日：2017-03-02

    申请号：US15308117

    申请日：2015-05-01

    申请人： SUNTORY HOLDINGS LIMITED

    发明人： Yoshikazu TANAKA ,  Naoko OKITSU ,  Keisuke MATSUI

    IPC分类号： C12N9/10 ,  C12P17/06

    CPC分类号： C12N9/1048 ,  A01H5/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/09 ,  C12P17/06 ,  C12P19/60 ,  C12P21/00 ,  C12Y204/00

    摘要： Provided is a polynucleotide for encoding a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone, particularly flavone 4′-glucoside. A polynucleotide selected from the group consisting of: (a) polynucleotides comprising a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5; (b) polynucleotides that hybridize, under stringent conditions, with polynucleotides comprising a base sequence complementary to a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5, wherein the polynucleotides encode a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; (c) polynucleotides for encoding proteins comprising an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6; (d) polynucleotides for encoding proteins comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted, and/or added in an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6, the polynucleotides exhibiting activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; and the like.

    摘要翻译： 本发明提供一种编码蛋白质的多核苷酸，其表现出将糖转移到黄酮，特别是黄酮4'-葡萄糖苷的第7位的羟基的活性。 选自以下的多核苷酸：（a）包含SEQ ID NO：1或SEQ ID NO：5的碱基序列的多核苷酸; （b）在严格条件下与包含与SEQ ID NO：1或SEQ ID NO：5的碱基序列互补的碱基序列的多核苷酸杂交的多核苷酸，其中所述多核苷酸编码显示转移糖的活性的蛋白质 到黄酮的第7位的羟基; （c）编码包含SEQ ID NO：2或SEQ ID NO：6的氨基酸序列的蛋白质的多核苷酸; （d）用于编码蛋白质的多核苷酸，其包含其中一个或多个氨基酸已被缺失，取代，插入和/或加入到SEQ ID NO：2或SEQ ID NO：6的氨基酸序列中的氨基酸序列 表现出将糖转移到黄酮第7位羟基的活性的多核苷酸; 等等。