公开(公告)号：US11913942B2

公开(公告)日：2024-02-27

申请号：US16924081

申请日：2020-07-08

申请人： IDEXX LABORATORIES, INC.

发明人： Murthy V S N Yerramilli ,  Hongzhi Xie ,  Daniel Wayne Patch ,  Giosi Farace

IPC分类号： G01N33/53 ,  G01N33/542 ,  G01N33/68 ,  C12N9/04

CPC分类号： G01N33/5308 ,  C12N9/0006 ,  G01N33/542 ,  G01N33/6812 ,  C12Y101/01049 ,  G01N2800/347

摘要： Homogeneous immunoassays that allow for compensation of background signals inherent in samples and reagents. The use of homogeneous immunoassays for the detection of the presence or amount of symmetrical Dimethyl Arginine (SDMA) in biological samples. Reagents and kits for conducting the assays.

公开(公告)号：US20240002867A1

公开(公告)日：2024-01-04

申请号：US17935466

申请日：2022-09-26

申请人： Jiangnan University

发明人： Zhiming RAO ,  Jiajia YOU ,  Taowei YANG ,  Xuewei PAN ,  Xian ZHANG ,  Meijuan XU

IPC分类号： C12N15/75 ,  C12N9/04 ,  C12N9/90 ,  C12N9/88 ,  C12N9/92 ,  C12N1/20 ,  C07K14/32 ,  C12P25/00

CPC分类号： C12N15/75 ,  C12N9/0006 ,  C12N9/90 ,  C12N9/88 ,  C12N9/92 ,  C12N1/205 ,  C07K14/32 ,  C12P25/00 ,  C12Y101/01049 ,  C12Y503/01006 ,  C12Y401/99012 ,  C12Y503/01009 ,  C12R2001/125

摘要： The present disclosure provides a Bacillus subtilis strain, a recombinant B. subtilis strain and use thereof, belongs to the technical field of microbial fermentation. B. subtilis strain RF1-6 provided by the present disclosure is a mutant strain with the highest riboflavin yield screened by gene modification and mutagenesis using a high riboflavin-producing strain RF1 as a starting strain, deposited at China Center for Type Culture Collection (CCTCC) under accession number M 2022565. Riboflavin yield can be increased by 22.8% compared with that of the high riboflavin-producing strain RF1.

公开(公告)号：US20170073661A1

公开(公告)日：2017-03-16

申请号：US15363038

申请日：2016-11-29

申请人： Roche Diabetes Care, Inc.

发明人： Wolfgang Roedel ,  Carina Horn ,  Nelli Steinke ,  Nadine Bucci ,  Thomas Meier ,  Rainer Schmuck ,  Rolf Nagel ,  Dieter Heindl

IPC分类号： C12N9/96 ,  C12Q1/26 ,  C12N9/02 ,  C12Q1/32 ,  C12N9/04

CPC分类号： C12N9/96 ,  C12N9/0006 ,  C12N9/0036 ,  C12Q1/26 ,  C12Q1/32 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01047 ,  C12Y101/01049 ,  C12Y106/05002 ,  C12Y106/99

摘要： Methods are provided for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme. In addition, enzymes are provided that are stabilized with a stabilized coenzyme, as well as the use thereof in test elements for detecting analytes. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.

摘要翻译： 提供了通过在稳定的辅酶的存在下储存酶来稳定酶的方法。 此外，提供了用稳定化的辅酶稳定的酶以及其用于检测分析物的测试元件中的用途。 其他方面包括独特的组合物，方法，技术，涉及酶稳定化的系统和装置。

公开(公告)号：US20150089683A1

公开(公告)日：2015-03-26

申请号：US14395636

申请日：2013-03-06

申请人： Monsanto Technology LLC

发明人： Mark Scott Abad ,  Pranesh Badami ,  Amarjit Basra ,  Erin Bell ,  Christopher P. Bonin ,  Paolo Castiglioni ,  Eric R. Cerny ,  Jaishree M. Chittoor-Vijayanath ,  Paul Chomet ,  Todd Dezwaan ,  Stephen Duff ,  Michael D. Edgerton ,  Barry S. Goldman ,  Balasulojini Karunanandaa ,  Kempagangaiah Krishnamurthy ,  Ping Li ,  Adrian A. Lund ,  Llinda Lutfiyya ,  Thomas M. Malvar ,  Donald E. Nelson ,  Monnanda S. Rajani ,  Dhanalakshmi Ramachandra ,  Thomas G. Ruff ,  Sara Salvador ,  S. Sangeetha ,  Char Shobha ,  Jeffrey M. Staub ,  Virginia Ursin ,  Tyamagondlu V. Venkatesh ,  Kammaradi R. Vidya ,  Xiaoyun Wu ,  Sean Yang ,  Jianmin Zhao

IPC分类号： C12N15/82 ,  C12N5/04 ,  A01H1/02

CPC分类号： C12N15/8261 ,  A01H1/02 ,  C07K14/415 ,  C07K14/81 ,  C12N5/04 ,  C12N9/0006 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/825 ,  C12N15/8271 ,  C12N15/8273 ,  C12Y101/01049 ,  Y02A40/146

摘要： This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

摘要翻译： 本公开提供具有增强性状的转基因植物，例如增加的产量，增加的氮利用效率和增强的耐旱性; 这些转基因植物的繁殖体，后代和田间作物; 以及制备和使用这些转基因植物的方法。 本公开还提供了从这种转基因植物产生杂种种子的方法，种植这种种子并选择具有增强特征的后代植物。 还公开了具有改变的表型的转基因植物，其可用于筛选和选择所需增强性状的转基因事件。

公开(公告)号：US20100120105A1

公开(公告)日：2010-05-13

申请号：US12606410

申请日：2009-10-27

申请人： Larry Cameron Anthony ,  Michael Dauner ,  Gail K. Donaldson ,  Brian James Paul

发明人： Larry Cameron Anthony ,  Michael Dauner ,  Gail K. Donaldson ,  Brian James Paul

IPC分类号： C12P7/04 ,  C12N1/00 ,  C12N1/21 ,  C12N1/19

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/18 ,  C12N9/88 ,  C12N9/92 ,  C12N15/52 ,  C12Y101/01044 ,  C12Y101/01049 ,  C12Y207/02011 ,  C12Y401/02014 ,  C12Y402/02012 ,  Y02E50/10

摘要： A microbial host cell is provided for the production of isobutanol. Carbon flux in the cell is optimized through the Entner-Doudoroff pathway.

摘要翻译： 提供用于生产异丁醇的微生物宿主细胞。 通过Entner-Doudoroff途径优化细胞中的碳通量。

公开(公告)号：US07078204B2

公开(公告)日：2006-07-18

申请号：US10312007

申请日：2001-06-15

申请人： Haruhiko Yokoi ,  Seiko Ando ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Shin-ichi Hashimoto

发明人： Haruhiko Yokoi ,  Seiko Ando ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Shin-ichi Hashimoto

IPC分类号： C12N9/04 ,  C12N1/20 ,  C12P21/04 ,  C12P13/04 ,  C07H21/04

CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/08 ,  C12Y101/01049

摘要： The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant.According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.

摘要翻译： 本发明涉及一种衍生自属于棒状杆菌属的细菌的新型葡萄糖-6-磷酸脱氢酶（以下称为“G6PD”），编码该酶的DNA，包含该DNA的重组DNA，包含重组体 DNA，包含其染色体上的DNA的转化体，以及生产L-氨基酸或G6PD的方法，其包括培养转化体。 根据本发明，获得了修饰的G6PD和编码G6PD的D​​NA，通过使用改良的G6PD可以提高微生物的L-氨基酸的生产率。

公开(公告)号：US20060014259A9

公开(公告)日：2006-01-19

申请号：US10091342

申请日：2002-03-06

申请人： Kevin Burke ,  Hermann Sahm ,  Lothar Eggeling ,  Bernd Moritz ,  L. Dunican ,  Ashling McCormack ,  Cliona Stapelton ,  Bettina Mockel ,  Georg Thierbach ,  Rita Dunican

发明人： Kevin Burke ,  Hermann Sahm ,  Lothar Eggeling ,  Bernd Moritz ,  L. Dunican ,  Ashling McCormack ,  Cliona Stapelton ,  Bettina Mockel ,  Georg Thierbach ,  Rita Dunican

IPC分类号： C12P13/08 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N9/0006 ,  C12N15/77 ,  C12P13/08 ,  C12Y101/01049

摘要： The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an amplified gene encoding the Zwischenferment protein.

公开(公告)号：US11884940B2

公开(公告)日：2024-01-30

申请号：US17550212

申请日：2021-12-14

申请人： Provivi, Inc.

发明人： Effendi Leonard ,  Micah Sheppard ,  Thomas Heel ,  Kathryn Christina Wu

IPC分类号： C12N9/04 ,  C12P7/64 ,  C12N9/02 ,  C12N9/18 ,  C12N15/52 ,  C12N15/81 ,  C12P7/6409

CPC分类号： C12N9/0006 ,  C12N9/0071 ,  C12N9/18 ,  C12N15/52 ,  C12N15/815 ,  C12P7/6409 ,  C12Y101/0104 ,  C12Y101/01042 ,  C12Y101/01044 ,  C12Y101/01049 ,  C12Y114/19005 ,  C12Y301/01031

摘要： The present application relates to methods to improve biomass or lipid production in a microorganism from one or more fatty acid and one or more simple carbon co-substrates. Produced lipids may include unsaturated C6-C24 fatty acids, alcohols, aldehydes, and acetates which may be useful as final products or precursors to insect pheromones, fragrances, flavors, and polymer intermediates. The application further relates to recombinant microorganisms modified for improved production of biomass or lipid, or improved lipid selectivity. Also provided are methods of producing one or more lipid using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally one or more of the product lipid.

公开(公告)号：US11739355B2

公开(公告)日：2023-08-29

申请号：US17048553

申请日：2019-04-19

申请人： Zymergen Inc.

发明人： Cara Ann Tracewell ,  Alexander Glennon Shearer ,  Michael Shareef Siddiqui ,  Steven M. Edgar ,  Nicolaus Herman ,  Murtaza Shabbir Hussain

IPC分类号： C12N15/80 ,  C12P17/10 ,  C12N1/18 ,  C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/77 ,  C12N15/81

CPC分类号： C12P17/10 ,  C12N1/18 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1077 ,  C12N9/88 ,  C12N15/77 ,  C12N15/81 ,  C12Y101/01023 ,  C12Y101/01049 ,  C12Y204/02017 ,  C12Y206/01009 ,  C12Y207/06001 ,  C12Y301/03015 ,  C12Y305/04019 ,  C12Y306/01031 ,  C12Y401/01022 ,  C12Y402/01019 ,  C12Y403/02 ,  C12Y503/01016

摘要： The present disclosure describes the engineering of microbial cells for fermentative production of histamine and provides novel engineered microbial cells and cultures, as well as related histamine production methods.

公开(公告)号：US20190211342A1

公开(公告)日：2019-07-11

申请号：US16362897

申请日：2019-03-25

申请人： Yeda Research and Development Co. Ltd.

发明人： Ron MILO ,  Niv ANTONOVSKY ,  Elad NOOR ,  Arren BAR-EVEN ,  Yehudit ZOHAR ,  Lior ZELCBUCH ,  Shmuel GLEIZER ,  Shira AMRAM

IPC分类号： C12N15/70 ,  C07K14/195 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C07K14/245 ,  C12N1/20

CPC分类号： C12N15/70 ,  C07K14/195 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1205 ,  C12N9/88 ,  C12N9/90 ,  C12Y101/01049 ,  C12Y203/03009 ,  C12Y207/01011 ,  C12Y207/01019 ,  C12Y401/01039 ,  C12Y402/01011 ,  C12Y504/02

摘要： A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.