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1.发明申请公开(公告)号：US20180223302A1
公开(公告)日：2018-08-09
申请号：US15737558
申请日：2016-06-22
Applicant: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Yeda Research and Development Co. Ltd., at the Weizmann Institute of Science
Inventor： Arren BAR-EVEN , Tobias ERB , Steffen LINDNER , Philippe MARLIERE , Dan S. TAWFIK
IPC: C12N15/82
CPC classification number: C12N15/8269 , C12N15/8261 , Y02A40/146
Abstract: The present invention relates to an organism, a tissue, a cell or an organelle expressing enzymes which allow the conversion of 2-phosphoglycolate (2-PG; also known as glycolate 2-phosphate) into an intermediate compound of the Calvin-Benson-Bassham Cycle (CBBC) without releasing CO2. The organism, tissue, cell or organelle of the invention may be genetically engineered, transgenic and/or transplastomic so as to express at least one enzyme which is involved in this conversion. The present invention further relates to an organism, tissue, cell or organelle which comprises/expresses at least one enzyme which is involved in this conversion. The present invention further relates to a method for producing an organism, tissue, cell or organelle of the invention. The present invention further relates to a method of enzymatically converting 2-PG into an intermediate compound of the CBBC without releasing CO2. The present invention further relates to the use of an organism, tissue, cell or organelle of the invention for enzymatically converting 2-PG into an intermediate compound of the CBBC without releasing CO2.
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公开(公告)号：US20160298103A1
公开(公告)日：2016-10-13
申请号：US15189021
申请日：2016-06-22
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Ron MILO , Arren BAR-EVEN , Elad NOOR
CPC classification number: C12N9/93 , C12N9/0006 , C12N9/0008 , C12N9/1018 , C12N9/1205 , C12N9/13 , C12N9/18 , C12N9/88 , C12N9/90 , C12N15/70 , C12N15/8245 , C12N15/8247 , C12N15/8269 , C12P7/40 , C12P7/42 , C12P7/46 , C12P11/00 , C12Y101/01059 , C12Y102/01018 , C12Y201/03001 , C12Y208/03001 , C12Y401/01031 , C12Y402/01017 , C12Y402/01054 , C12Y604/01001 , C12Y604/01002 , G06F19/12 , Y02P20/52
Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.
Abstract translation: 提供了一种用于碳固定的系统。 该系统包括催化碳固定通道的反应的酶，其中碳固定通路的至少一个反应是羧化反应，其中碳固定路径的反应产物包括草酰乙酸酯和丙二酰辅酶A，其中酶 进行羧化反应的选自苯丙醇丙酮酸（PEP）羧基酶，丙酮酸羧化酶和乙酰辅酶A羧化酶，其中碳固定路径的出口产物是乙醛酸盐。 还提供了另外的碳固定路径和产生它们的方法。
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公开(公告)号：US20190211342A1
公开(公告)日：2019-07-11
申请号：US16362897
申请日：2019-03-25
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Ron MILO , Niv ANTONOVSKY , Elad NOOR , Arren BAR-EVEN , Yehudit ZOHAR , Lior ZELCBUCH , Shmuel GLEIZER , Shira AMRAM
IPC: C12N15/70 , C07K14/195 , C12N9/04 , C12N9/10 , C12N9/12 , C12N9/88 , C12N9/90 , C07K14/245 , C12N1/20
CPC classification number: C12N15/70 , C07K14/195 , C07K14/245 , C12N1/20 , C12N9/0006 , C12N9/1025 , C12N9/1205 , C12N9/88 , C12N9/90 , C12Y101/01049 , C12Y203/03009 , C12Y207/01011 , C12Y207/01019 , C12Y401/01039 , C12Y402/01011 , C12Y504/02
Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.
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公开(公告)号：US20240117386A1
公开(公告)日：2024-04-11
申请号：US17768228
申请日：2020-10-22
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Ron MILO , Shmuel GLEIZER , Niv ANTONOVSKY , Elad NOOR , Arren BAR-EVEN , Yehudit ZOHAR , Roee BEN NISSAN , Elad HERZ , Yinon Moise BAR-ON
CPC classification number: C12P1/04 , C07K14/195 , C12N1/20 , C12N9/0008 , C12N9/1205 , C12N9/88 , C12Y102/02001 , C12Y207/01019 , C12Y401/01039 , C12Y402/01001
Abstract: A recombinant bacteria which is genetically modified to express formate dehydrogenase (FDH), phosphoribulokinase (prk) and Ribulose-Bisphosphate Carboxylase/oxygenase (RuBisCo) is disclosed. The bacteria may be modified to be autotrophic.
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公开(公告)号：US20170183665A1
公开(公告)日：2017-06-29
申请号：US15312734
申请日：2015-05-21
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Ron MILO , Niv ANTONOVSKY , Elad NOOR , Arren BAR-EVEN , Yehudit ZOHAR , Lior ZELCBUCH , Shmuel GLEIZER , Shira AMRAM
CPC classification number: C12N15/70 , C07K14/195 , C07K14/245 , C12N1/20 , C12N9/0006 , C12N9/1025 , C12N9/1205 , C12N9/88 , C12N9/90 , C12Y101/01049 , C12Y203/03009 , C12Y207/01011 , C12Y207/01019 , C12Y401/01039 , C12Y402/01011 , C12Y504/02
Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.
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6.发明申请
公开(公告)号：US20170081682A1
公开(公告)日：2017-03-23
申请号：US15101097
申请日：2014-12-10
Applicant: Yeda Research and Development Co. Ltd.
Inventor： Arren BAR-EVEN , Ron MILO , Elad NOOR , Lior ZELCBUCH
IPC: C12P7/40 , C12N1/20 , C12N9/10 , C12P7/26 , C12P7/16 , C12P5/02 , C12P5/00 , C12P7/64 , C12N15/52 , C12P7/06
CPC classification number: C12P7/40 , C12N1/20 , C12N9/1029 , C12N15/52 , C12P5/007 , C12P5/023 , C12P5/026 , C12P7/06 , C12P7/16 , C12P7/26 , C12P7/649 , C12Y203/01054 , Y02E50/343
Abstract: An isolated microorganism is disclosed being genetically modified to express pyruvate formate lyase (PFL) or 2-ketobutyrate formate lyase, wherein acetyl-CoA of the microorganism is converted to pyruvate in the presence of formate in a single step reaction, wherein the net flux of the reaction is in the direction of pyruvate synthesis.Uses of the microorganism and products comprising same are also disclosed.
