公开(公告)号：US20240011067A1

公开(公告)日：2024-01-11

申请号：US18254531

申请日：2021-11-30

Applicant: Universiteit Antwerpen

Inventor： Sarah LEBEER ,  Sarah AHANNACH ,  Irina SPACOVA ,  Stijn WITTOUCK

IPC: C12P25/00 ,  C12N9/78 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/12

CPC classification number: C12P25/00 ,  C12N9/78 ,  C12Y305/04026 ,  C12N9/0006 ,  C12Y101/01193 ,  C12N9/1085 ,  C12Y205/01009 ,  C12Y205/01078 ,  C12N9/88 ,  C12Y401/99012 ,  C12N9/1241 ,  C12Y207/07002 ,  C12N9/1205 ,  C12Y207/01026

Abstract: The present invention to the identification of a novel isolated bacterial strain, the Limosilactobacillus reuteri strain which is characterized by a naturally occurring overproduction of riboflavin (vitamin B2) compared to other known strains of lactobacilli. Said strain of the Limosilactobacillus species is deposited under accession number LMG P-32020. In a further aspect, also the uses of said L. reuteri strain are disclosed herein. In particular, the use of said strain in food and feed industry, human and veterinary health, large-scale vitamin production, cosmetics and consumer care products is disclosed.

公开(公告)号：US20240002867A1

公开(公告)日：2024-01-04

申请号：US17935466

申请日：2022-09-26

Applicant: Jiangnan University

Inventor： Zhiming RAO ,  Jiajia YOU ,  Taowei YANG ,  Xuewei PAN ,  Xian ZHANG ,  Meijuan XU

IPC: C12N15/75 ,  C12N9/04 ,  C12N9/90 ,  C12N9/88 ,  C12N9/92 ,  C12N1/20 ,  C07K14/32 ,  C12P25/00

CPC classification number: C12N15/75 ,  C12N9/0006 ,  C12N9/90 ,  C12N9/88 ,  C12N9/92 ,  C12N1/205 ,  C07K14/32 ,  C12P25/00 ,  C12Y101/01049 ,  C12Y503/01006 ,  C12Y401/99012 ,  C12Y503/01009 ,  C12R2001/125

Abstract: The present disclosure provides a Bacillus subtilis strain, a recombinant B. subtilis strain and use thereof, belongs to the technical field of microbial fermentation. B. subtilis strain RF1-6 provided by the present disclosure is a mutant strain with the highest riboflavin yield screened by gene modification and mutagenesis using a high riboflavin-producing strain RF1 as a starting strain, deposited at China Center for Type Culture Collection (CCTCC) under accession number M 2022565. Riboflavin yield can be increased by 22.8% compared with that of the high riboflavin-producing strain RF1.

公开(公告)号：US20190256797A1

公开(公告)日：2019-08-22

申请号：US16402662

申请日：2019-05-03

Applicant: DSM IP Assets B.V.

Inventor： Craig M. RUECKER ,  Swithin Patrick ADU-PEASAH ,  Brian S. ENGELHARDT ,  George T. VEEDER, III

IPC: C11B3/00 ,  C12P33/00 ,  C12P25/00 ,  C12P23/00 ,  C12P7/64 ,  C11B1/02 ,  C12N1/06

Abstract: The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

公开(公告)号：US20080311617A1

公开(公告)日：2008-12-18

申请号：US12091279

申请日：2006-10-25

Applicant: Michael Hans ,  Dietmar Laudert ,  Hans-Peter Hohmann ,  Martin Lehmann

Inventor： Michael Hans ,  Dietmar Laudert ,  Hans-Peter Hohmann ,  Martin Lehmann

IPC: C12P25/00 ,  C12N1/21

CPC classification number: C12N9/1022 ,  C12P17/12 ,  C12P25/00

Abstract: The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B2), FAD, FMN, pyridoxal phosphate (vitamin B6), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them.

Abstract translation: 本发明涉及核糖-5-磷酸，核酮糖-5-磷酸或木酮糖-5-磷酸是生物合成前体如核黄素（维生素B2），FAD，FMN，吡哆醛磷酸酯（ 维生素B6），鸟苷，GMP，腺苷，AMP。 本发明还涉及产生这些化合物的生物体的产生。 此外，还涉及产生突变的转酮酶，其允许在葡萄糖上正常生长，但当被引入生产菌株中时葡萄糖酸盐的生长减少，以及编码它们的多核苷酸。

公开(公告)号：US06878536B2

公开(公告)日：2005-04-12

申请号：US10076157

申请日：2002-02-15

Applicant: Markus Pompejus ,  Harald Seulberger ,  Hans Wolfgang Höffken ,  Jose Luis Revuelta Doval ,  Alberto Jimenez ,  Maria Angeles Santos Garcia

Inventor： Markus Pompejus ,  Harald Seulberger ,  Hans Wolfgang Höffken ,  Jose Luis Revuelta Doval ,  Alberto Jimenez ,  Maria Angeles Santos Garcia

IPC: C12N15/09 ,  C07K14/39 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N15/00 ,  C12N15/52 ,  C12N15/53 ,  C12N15/80 ,  C12P19/28 ,  C12P25/00 ,  C12R1/645

CPC classification number: C12N9/93 ,  C12N9/0006 ,  C12N9/1077 ,  C12N9/1235 ,  C12P25/00

Abstract: Genes of purine biosynthesis from Ashbya gossypii are used in microbial riboflavin synthesis.

Abstract translation: 用于生物合成核黄素的嘌呤生物合成基因。

公开(公告)号：US20050053932A1

公开(公告)日：2005-03-10

申请号：US10483227

申请日：2002-07-10

Applicant: Marvin Karos ,  Henning Althofer ,  Burkhard Kroger ,  Jose Revuelta Doval

Inventor： Marvin Karos ,  Henning Althofer ,  Burkhard Kroger ,  Jose Revuelta Doval

IPC: C07K14/37 ,  C07K14/47 ,  C12N1/15 ,  C12N15/30 ,  C12P25/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/02

CPC classification number: C12P25/00 ,  C07K14/37 ,  C07K14/47

Abstract: The present invention relates to novel polynucleotides from Ashbya gossypii; to oligonucleotides hybridizing therewith; to expression cassettes and vectors which comprise these polynucleotides; to microorganisms transformed therewith; to polypeptides encoded by these polynucleotides; and to the use of the novel polypeptides and polynucleotides as targets for improving stress resistance and, in particular, improving vitamin B2 production in microorganisms of the genus Ashbya.

Abstract translation: 本发明涉及来自棉铃癣菌的新型多核苷酸; 与其杂交的寡核苷酸; 包含这些多核苷酸的表达盒和载体; 对其进行转化的微生物; 涉及由这些多核苷酸编码的多肽; 以及使用新型多肽和多核苷酸作为改善抗逆性的目标，特别是改善Ashbya属微生物中的维生素B2产生。

公开(公告)号：US5837528A

公开(公告)日：1998-11-17

申请号：US384626

申请日：1995-02-06

Applicant: John B. Perkins ,  Alan Sloma ,  Janice G. Pero

Inventor： John B. Perkins ,  Alan Sloma ,  Janice G. Pero

IPC: C12N1/21 ,  C12N15/31 ,  C12N15/52 ,  C12P25/00

CPC classification number: C12P25/00 ,  C12N15/52

Abstract: Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.

公开(公告)号：US5037981A

公开(公告)日：1991-08-06

申请号：US515962

申请日：1990-04-27

Applicant: Masayasu Kurono ,  Takehiko Suzuki ,  Tomio Ogasawara ,  Nobuko Ohishi ,  Kunio Yagi

Inventor： Masayasu Kurono ,  Takehiko Suzuki ,  Tomio Ogasawara ,  Nobuko Ohishi ,  Kunio Yagi

IPC: C07D405/12 ,  C07D475/04 ,  C12P25/00

CPC classification number: C07D405/12 ,  C07D475/04 ,  C12P25/00

Abstract: The present invention relates to intermediates for synthesizing BH.sub.4 and derivatives thereof. The intermediates are shown as follows; ##STR1## wherein R.sub.1 is a hydrogen atom, alkyl, aralkyl, or aryl group; R.sub.2 is an alkyl, hydroxyalkyl, or polyhydroxyalkyl group; R.sub.3 and R.sub.4 are the same or different and represent alkyl, aralkyl, or aryl group; R.sub.5, R.sub.6, R.sub.7, and R.sub.8 are the same or different and represent a hydrogen atom or acyl group; R.sub.9 is an alkyl, aralkyl, or aryl group; R.sub.10 and R.sub.11 are the same or different and represent a hydrogen atom or acyl group; n is an integer of 5 or less; and HX is an acid. The invention also relates to a process for the preparation of L-biopterin.

公开(公告)号：US4165250A

公开(公告)日：1979-08-21

申请号：US850991

申请日：1977-11-14

Applicant: Albert Epstein ,  Glen Graham ,  William A. Sklarz

Inventor： Albert Epstein ,  Glen Graham ,  William A. Sklarz

IPC: A23K1/16 ,  C07D475/14 ,  C12P25/00 ,  C12D5/04

CPC classification number: C07D475/14 ,  A23K20/174 ,  C12P25/00

Abstract: Riboflavin is recovered from fermentation broth by diluting the broth with a predetermined quantity of water, centrifuging the diluted broth to form a sludge, resuspending the sludge with a predetermined quantity of water, and centrifuging the resuspended sludge to yield a product usuable directly as an animal feed supplement or suitable for further purification for pharmaceutical use. Optionally the broth or resuspended sludge is treated with an enzyme before centrifugation.

Abstract translation: 通过用预定量的水稀释培养液从发酵液中回收核黄素，将稀释的肉汤离心以形成污泥，用预定量的水重新悬浮污泥，并将再悬浮的污泥离心以产生直接作为动物使用的产品 饲料补充剂或适合用于药物用途的进一步纯化。 任选地，在离心之前用酶处理肉汤或再悬浮的污泥。

公开(公告)号：US3647627A

公开(公告)日：1972-03-07

申请号：US3647627D

申请日：1968-07-12

Applicant: KYOWA HAKKO KOGYO KK

Inventor： TANAKA MASAO ,  NAKAMURA NOBUO ,  TAKASAWA SEIGO

IPC: C12P25/00 ,  C12D13/06

CPC classification number: C12P25/00 ,  Y10S435/84 ,  Y10S435/843 ,  Y10S435/85 ,  Y10S435/859 ,  Y10S435/861

Abstract: FLAVIN-ADINUCLEOTIDE IS PRODUCED BY FERMENTATION. A MICROORGANISM OF BREVIBACTERIUM, MICROCOCCUS, CORYNEBACTERIUM OR FLAVOBACTERIUM IS CULTURED IN A AQUEOUS MEDIUM UNDER AEROBIC CONDITIONS AT 20*-40*C. THE PRODUCT IS RECOVERED BY ION-EXCHANGE RESIN TREATMENT.