公开(公告)号：US20080307548A1

公开(公告)日：2008-12-11

申请号：US11573010

申请日：2005-08-01

申请人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Satoko Akatsuka

发明人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Satoko Akatsuka

IPC分类号： C12N15/11 ,  C12N1/21 ,  C12N1/19 ,  C12N5/10 ,  A01H5/00 ,  C07K14/00 ,  C12N15/63

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： It is intended to provide a gene encoding a protein having an activity of improving a tolerance to an environmental stress such as salt, moisture, or heat stress, in particular, a tolerance to water stress; a protein having an activity of improving a tolerance to an environmental stress; a transgenic plant having an enhanced tolerance to an environmental stress, and so on. A halophyte Salicornia europaea, which has a strong activity of improving a tolerance to water stress and can grow even in a dry area with much salt accumulation, was used to search genes participating in the salt tolerance of Salicornia europaea by a functional screening method using an Escherichia coli gene expression system, with the aim of isolating genes (cDNAs) participating in the salt-tolerance mechanism of Salicornia europaea. As a result, it was found out that an arabinogalactan protein consisting of 427 amino acids and having partial homology to a Fasciclin-like arabinogalactan protein of Arabidopsis thaliana has a function of enhancing salt tolerance, water stress tolerance (sorbitol tolerance), and heat tolerance.

摘要翻译： 旨在提供编码具有改善对诸如盐，水分或热应激等环境胁迫的耐受性的活性的蛋白质的基因，特别是对水分胁迫的耐受性; 具有改善对环境胁迫耐受性的活性的蛋白质; 具有增强的对环境胁迫耐受性的转基因植物等等。 使用具有强烈的抗水分胁迫活性的盐藻（Salicornia europaea），甚至可以在具有大量盐积累的干燥区域中生长的活性，通过使用功能筛选方法来搜索参与耐盐性菌的基因 大肠杆菌基因表达系统，目的是分离参与Salicornia europaea耐盐机制的基因（cDNA）。 结果发现，由427个氨基酸组成并与拟南芥的Fasciclin样阿拉伯半乳聚糖蛋白具有部分同源性的阿拉伯半乳聚糖蛋白具有增强耐盐性，耐水胁迫耐受性（山梨醇耐受性）和耐热性的功能 。

公开(公告)号：US20060288438A1

公开(公告)日：2006-12-21

申请号：US11446828

申请日：2006-06-05

申请人： Yoshihiro Ozeki ,  Takashi Fukuda ,  Mikiko Oyanagi ,  Takatoshi Koda

发明人： Yoshihiro Ozeki ,  Takashi Fukuda ,  Mikiko Oyanagi ,  Takatoshi Koda

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8201 ,  C07K14/415

摘要： The invention provides novel, carrot-derived MITE-like elements (transposable elements). It further provides transcriptional activation elements comprising at least one transposable element, in particular one of the above MITE-like elements. Specifically, it provides transcriptional activation elements having a DNA comprising the nucleotide sequence shown under SEQ ID NO:1 or a functional equivalent thereto and/or a DNA comprising the nucleotide sequence shown under SEQ ID NO:2 or a functional equivalent thereto. The transcriptional activation elements of the invention can increase or activate the reduced expression of a foreign gene introduced by the transgenic technology. Therefore, the transcriptional activation elements contribute to stable expression of a foreign gene introduced in a plant genome and are useful in stably producing genetically modified plants.

摘要翻译： 本发明提供了新颖的，由胡萝卜衍生的MITE样元件（转座元件）。 其进一步提供转录激活元件，其包含至少一个转座元件，特别是上述MITE样元件之一。 具体地说，它提供具有包含SEQ ID NO：1所示核苷酸序列或其功能等同物的DNA的转录激活元件和/或包含SEQ ID NO：2所示核苷酸序列或其功能等同物的DNA。 本发明的转录激活元件可以增加或激活由转基因技术引入的外源基因的降低的表达。 因此，转录激活元件有助于在植物基因组中引入的外源基因的稳定表达，并且可用于稳定生产转基因植物。

公开(公告)号：US5916380A

公开(公告)日：1999-06-29

申请号：US723989

申请日：1996-09-30

申请人： Norio Yuki ,  Masazumi Mori ,  Yoshihiro Ozeki

发明人： Norio Yuki ,  Masazumi Mori ,  Yoshihiro Ozeki

IPC分类号： C22C19/03 ,  C22C30/00 ,  C22C38/00 ,  C22C38/04 ,  C22C38/08 ,  H01J9/14 ,  H01J29/48

CPC分类号： C22C19/03 ,  C22C30/00 ,  C22C38/04 ,  C22C38/08 ,  H01J29/485 ,  H01J9/14

摘要： An Fe--Ni alloy for use as a part 10 of an electron-gun 4 is press-blanked by a punch to form minute apertures 10a, 10b, 10c for passing an electron beam 3. The burrs 10 formed around the minute apertures 10a, 10b, 10c are detrimental to such part 4. The Fe--Ni alloy according to the present invention essentially consists of from 30 to 55 wt % of Ni, not more than 0.5 wt % of Si, not more than 1.5 wt % of Mn, and the balance being Fe and unavoidable impurities. The alloy includes from 10 to 1,000 of A type or B type non-metallic inclusions of 10 .mu.m or more in length per 1 mm.sup.2 of longitudinal cross section, and from 100 to 50,000 of C type non-metallic inclusions having a diameter of 5 .mu.m or less.

摘要翻译： 用作电子枪4的第10部分的Fe-Ni合金通过冲头冲裁以形成用于通过电子束3的微小孔10a，10b，10c。形成在微小孔10a， 10b，10c对于这种部件4是有害的。根据本发明的Fe-Ni合金基本上由30-55wt％的Ni，不大于0.5wt％的Si，不超过1.5wt％的Mn， 余量为Fe和不可避免的杂质。 该合金包括每1mm 2纵向截面长度为10μm或更大的A型或B型非金属夹杂物的10至1,000个，直径为5的C型非金属夹杂物为100至50,000个 亩以下。

公开(公告)号：US20120135469A1

公开(公告)日：2012-05-31

申请号：US13389233

申请日：2010-01-21

申请人： Yoshihiro Ozeki ,  Nobuhiro Sasaki ,  Kazuo Nagasawa ,  Masayuki Tera ,  Yuki Matsuba ,  Haruka Nakamura ,  Yutaka Abe

发明人： Yoshihiro Ozeki ,  Nobuhiro Sasaki ,  Kazuo Nagasawa ,  Masayuki Tera ,  Yuki Matsuba ,  Haruka Nakamura ,  Yutaka Abe

IPC分类号： C12P19/44 ,  C12N5/10 ,  A01H5/00 ,  C07H15/207 ,  C12N15/54 ,  C12N9/10 ,  C12N15/63

CPC分类号： C12N9/1048 ,  C07H13/08 ,  C07H15/18 ,  C07H17/065 ,  C12P19/44 ,  C12P19/60

摘要： An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A): wherein R1 is independently selected from hydrogen, or C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO2, and SO2, n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a β bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.

摘要翻译： 本发明的目的是提供一种含糖糖核苷酸以外的糖供体化合物和能够使用糖核苷酸以外的糖供体化合物催化糖基转移反应的酶的供糖试剂。 本发明提供以下：含有式（A）化合物的给糖试剂：其中R 1独立地选自氢或C 1-6烷基，C 2-6烯基和C 2-6炔基，其中每个基团 未取代或被一个或多个选自OH，F，Cl，Br，I，CN，NO2和SO2的基团取代，n为0,1,2,3,4或5，m为0或1，X 代表通过＆bgr结合的单糖; 其端基异碳碳键; 能够使用糖供体催化糖基转移反应的糖基转移酶; 和包含编码糖基转移酶的DNA的糖基转移酶基因。

公开(公告)号：US20060110087A1

公开(公告)日：2006-05-25

申请号：US10543781

申请日：2003-11-17

申请人： Toshiro Igari ,  Yoshihiro Ozeki

发明人： Toshiro Igari ,  Yoshihiro Ozeki

IPC分类号： F16C13/00

CPC分类号： F16C19/186 ,  B23B5/02 ,  B60B3/04 ,  B60B27/00 ,  B60B27/0005 ,  B60B27/0026 ,  B60B27/0084 ,  B60B27/0094 ,  F16C33/64 ,  F16C2326/02

摘要： Both end portions in an axial direction of a hub 8b are supported by a supporting portion of a working apparatus rotatably centering on a center axis of the hub 8b and in a state of restraining a displacement thereof in a radial direction. Further, before removing the hub 8b from the supporting portion from the state, an outer side face 28 of an attaching flange 13 and a first inner ring track 14 are worked to predetermined shapes. Thereby, judder in braking is prevented from being brought about by promoting a perpendicularity of the outer side face 28 of the attaching flange 13 provided to the hub 8b relative to a rotational center of the hub 8b and restraining a deflection of a rotor coupled to fix to the outer side face 28.

摘要翻译： 轮毂8b的轴向两端部由作业装置的支承部支承，以可枢转为中心的轮毂8b的中心轴线，并且在径向上限制其位移的状态。 此外，在从该状态从支撑部分移除轮毂8b之前，将安装凸缘13的外侧面28和第一内圈轨道14加工成预定的形状。 因此，通过促进设置在轮毂8b上的安装凸缘13的外侧面28相对于轮毂8b的旋转中心的垂直度，从而防止制动中的抖动，并且限制转子的偏转 以固定到外侧面28。

公开(公告)号：US08877918B2

公开(公告)日：2014-11-04

申请号：US13362373

申请日：2012-01-31

申请人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

发明人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

IPC分类号： C07H21/00 ,  C12N15/10 ,  C12N1/06 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N1/06 ,  C12N15/1003 ,  C12Q2527/101 ,  C12Q2527/113

摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.

摘要翻译： 提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。 核酸提取方法包括以下步骤：将细胞悬浮液引入容器，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤 加热器加热至设定温度，从而将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃，容器保持密封状态。

公开(公告)号：US20120197008A1

公开(公告)日：2012-08-02

申请号：US13362373

申请日：2012-01-31

申请人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

发明人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

IPC分类号： C07H1/08

CPC分类号： C12Q1/6806 ,  C12N1/06 ,  C12N15/1003 ,  C12Q2527/101 ,  C12Q2527/113

摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.

摘要翻译： 提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。 核酸提取方法包括以下步骤：将细胞悬浮液引入容器中，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤 加热器加热到设定温度，由此将容器保持在密封状态下，将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃。

公开(公告)号：US07816582B2

公开(公告)日：2010-10-19

申请号：US11573010

申请日：2005-08-01

申请人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Satoko Akatsuka

发明人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Satoko Akatsuka

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/00 ,  C12N15/29

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： It is intended to provide a gene encoding a protein having an activity of improving a tolerance to an environmental stress such as salt, moisture, or heat stress, in particular, a tolerance to water stress; a protein having an activity of improving a tolerance to an environmental stress; a transgenic plant having an enhanced tolerance to an environmental stress, and so on. A halophyte Salicornia europaea, which has a strong activity of improving a tolerance to water stress and can grow even in a dry area with much salt accumulation, was used to search genes participating in the salt tolerance of Salicornia europaea by a functional screening method using an Escherichia coli gene expression system, with the aim of isolating genes (cDNAs) participating in the salt-tolerance mechanism of Salicornia europaea. As a result, it was found out that an arabinogalactan protein consisting of 427 amino acids and having partial homology to a Fasciclin-like arabinogalactan protein of Arabidopsis thaliana has a function of enhancing salt tolerance, water stress tolerance (sorbitol tolerance), and heat tolerance.

摘要翻译： 旨在提供编码具有改善对诸如盐，水分或热应激等环境胁迫的耐受性的活性的蛋白质的基因，特别是对水分胁迫的耐受性; 具有改善对环境胁迫耐受性的活性的蛋白质; 具有增强的对环境胁迫耐受性的转基因植物等等。 使用具有强烈的抗水分胁迫活性的盐藻（Salicornia europaea），甚至可以在具有大量盐积累的干燥区域中生长的活性，通过使用功能筛选方法来搜索参与耐盐性菌的基因 大肠杆菌基因表达系统，目的是分离参与Salicornia europaea耐盐机制的基因（cDNA）。 结果发现，由427个氨基酸组成并与拟南芥的Fasciclin样阿拉伯半乳聚糖蛋白具有部分同源性的阿拉伯半乳聚糖蛋白具有增强耐盐性，耐水胁迫耐受性（山梨醇耐受性）和耐热性的功能 。

公开(公告)号：US07151202B1

公开(公告)日：2006-12-19

申请号：US10031331

申请日：2000-07-19

申请人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Takeo Saito

发明人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Takeo Saito

IPC分类号： C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N1/04 ,  A01K2217/05 ,  C07K14/415 ,  C12N15/1034 ,  C12N15/8273 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158

摘要： The present invention relates to isolated DNA sequences from halophytes encoding proteins for improving tolerance to environmental stresses in plants. The invention also relates to vectors comprising the polynucleotides, and transformed host cells, and plants with improved environmental stress tolerance.

摘要翻译： 本发明涉及来自编码蛋白质的盐藻的分离的DNA序列，用于改善植物对环境胁迫的耐受性。 本发明还涉及包含多核苷酸和转化的宿主细胞的载体以及具有改善的环境胁迫耐受性的植物。

公开(公告)号：US5849420A

公开(公告)日：1998-12-15

申请号：US579010

申请日：1995-12-27

申请人： Kiyoaki Nishikawa ,  Yoshihiro Ozeki ,  Masazumi Mori

发明人： Kiyoaki Nishikawa ,  Yoshihiro Ozeki ,  Masazumi Mori

IPC分类号： C22C38/00 ,  C22C38/40 ,  C22C38/58

CPC分类号： C22C38/40 ,  Y10T428/12361

摘要： A punched part consists of an Fe--Cr--Ni alloy essentially consisting of from 15 to 20% of Cr, from 9 to 15% of Ni, from 0.001 to 0.0050% of S, the balance being Fe and unavoidable impurities. The burrs formed during punching are suppressed due to addition of S to the Fe--Cr--Ni alloy.

摘要翻译： 冲压部分由基本上由15％至20％的Cr，9至15％的Ni，0.001至0.0050％的S，余量为Fe和不可避免的杂质组成的Fe-Cr-Ni合金组成。 由于在Fe-Cr-Ni合金中添加S，所以在冲压时形成的毛刺被抑制。