公开(公告)号：US20080307548A1

公开(公告)日：2008-12-11

申请号：US11573010

申请日：2005-08-01

申请人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Satoko Akatsuka

发明人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Satoko Akatsuka

IPC分类号： C12N15/11 ,  C12N1/21 ,  C12N1/19 ,  C12N5/10 ,  A01H5/00 ,  C07K14/00 ,  C12N15/63

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： It is intended to provide a gene encoding a protein having an activity of improving a tolerance to an environmental stress such as salt, moisture, or heat stress, in particular, a tolerance to water stress; a protein having an activity of improving a tolerance to an environmental stress; a transgenic plant having an enhanced tolerance to an environmental stress, and so on. A halophyte Salicornia europaea, which has a strong activity of improving a tolerance to water stress and can grow even in a dry area with much salt accumulation, was used to search genes participating in the salt tolerance of Salicornia europaea by a functional screening method using an Escherichia coli gene expression system, with the aim of isolating genes (cDNAs) participating in the salt-tolerance mechanism of Salicornia europaea. As a result, it was found out that an arabinogalactan protein consisting of 427 amino acids and having partial homology to a Fasciclin-like arabinogalactan protein of Arabidopsis thaliana has a function of enhancing salt tolerance, water stress tolerance (sorbitol tolerance), and heat tolerance.

摘要翻译： 旨在提供编码具有改善对诸如盐，水分或热应激等环境胁迫的耐受性的活性的蛋白质的基因，特别是对水分胁迫的耐受性; 具有改善对环境胁迫耐受性的活性的蛋白质; 具有增强的对环境胁迫耐受性的转基因植物等等。 使用具有强烈的抗水分胁迫活性的盐藻（Salicornia europaea），甚至可以在具有大量盐积累的干燥区域中生长的活性，通过使用功能筛选方法来搜索参与耐盐性菌的基因 大肠杆菌基因表达系统，目的是分离参与Salicornia europaea耐盐机制的基因（cDNA）。 结果发现，由427个氨基酸组成并与拟南芥的Fasciclin样阿拉伯半乳聚糖蛋白具有部分同源性的阿拉伯半乳聚糖蛋白具有增强耐盐性，耐水胁迫耐受性（山梨醇耐受性）和耐热性的功能 。

公开(公告)号：US07816582B2

公开(公告)日：2010-10-19

申请号：US11573010

申请日：2005-08-01

申请人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Satoko Akatsuka

发明人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Satoko Akatsuka

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/00 ,  C12N15/29

CPC分类号： C12N15/8273 ,  C07K14/415

摘要： It is intended to provide a gene encoding a protein having an activity of improving a tolerance to an environmental stress such as salt, moisture, or heat stress, in particular, a tolerance to water stress; a protein having an activity of improving a tolerance to an environmental stress; a transgenic plant having an enhanced tolerance to an environmental stress, and so on. A halophyte Salicornia europaea, which has a strong activity of improving a tolerance to water stress and can grow even in a dry area with much salt accumulation, was used to search genes participating in the salt tolerance of Salicornia europaea by a functional screening method using an Escherichia coli gene expression system, with the aim of isolating genes (cDNAs) participating in the salt-tolerance mechanism of Salicornia europaea. As a result, it was found out that an arabinogalactan protein consisting of 427 amino acids and having partial homology to a Fasciclin-like arabinogalactan protein of Arabidopsis thaliana has a function of enhancing salt tolerance, water stress tolerance (sorbitol tolerance), and heat tolerance.

摘要翻译： 旨在提供编码具有改善对诸如盐，水分或热应激等环境胁迫的耐受性的活性的蛋白质的基因，特别是对水分胁迫的耐受性; 具有改善对环境胁迫耐受性的活性的蛋白质; 具有增强的对环境胁迫耐受性的转基因植物等等。 使用具有强烈的抗水分胁迫活性的盐藻（Salicornia europaea），甚至可以在具有大量盐积累的干燥区域中生长的活性，通过使用功能筛选方法来搜索参与耐盐性菌的基因 大肠杆菌基因表达系统，目的是分离参与Salicornia europaea耐盐机制的基因（cDNA）。 结果发现，由427个氨基酸组成并与拟南芥的Fasciclin样阿拉伯半乳聚糖蛋白具有部分同源性的阿拉伯半乳聚糖蛋白具有增强耐盐性，耐水胁迫耐受性（山梨醇耐受性）和耐热性的功能 。

公开(公告)号：US07151202B1

公开(公告)日：2006-12-19

申请号：US10031331

申请日：2000-07-19

申请人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Takeo Saito

发明人： Akiyo Yamada ,  Yoshihiro Ozeki ,  Takeo Saito

IPC分类号： C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N1/04 ,  A01K2217/05 ,  C07K14/415 ,  C12N15/1034 ,  C12N15/8273 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158

摘要： The present invention relates to isolated DNA sequences from halophytes encoding proteins for improving tolerance to environmental stresses in plants. The invention also relates to vectors comprising the polynucleotides, and transformed host cells, and plants with improved environmental stress tolerance.

摘要翻译： 本发明涉及来自编码蛋白质的盐藻的分离的DNA序列，用于改善植物对环境胁迫的耐受性。 本发明还涉及包含多核苷酸和转化的宿主细胞的载体以及具有改善的环境胁迫耐受性的植物。

公开(公告)号：US08877918B2

公开(公告)日：2014-11-04

申请号：US13362373

申请日：2012-01-31

申请人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

发明人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

IPC分类号： C07H21/00 ,  C12N15/10 ,  C12N1/06 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N1/06 ,  C12N15/1003 ,  C12Q2527/101 ,  C12Q2527/113

摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.

摘要翻译： 提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。 核酸提取方法包括以下步骤：将细胞悬浮液引入容器，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤 加热器加热至设定温度，从而将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃，容器保持密封状态。

公开(公告)号：US20120197008A1

公开(公告)日：2012-08-02

申请号：US13362373

申请日：2012-01-31

申请人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

发明人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

IPC分类号： C07H1/08

CPC分类号： C12Q1/6806 ,  C12N1/06 ,  C12N15/1003 ,  C12Q2527/101 ,  C12Q2527/113

摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.

摘要翻译： 提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。 核酸提取方法包括以下步骤：将细胞悬浮液引入容器中，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤 加热器加热到设定温度，由此将容器保持在密封状态下，将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃。