公开(公告)号：US20130059340A1

公开(公告)日：2013-03-07

申请号：US13696782

申请日：2011-05-19

Applicant: Giang Hai Tran ,  Tom Desmet ,  Wim Soetaert

Inventor： Giang Hai Tran ,  Tom Desmet ,  Wim Soetaert

IPC: C12P19/44

CPC classification number: C12N9/1051 ,  C12P19/18 ,  C12P19/44 ,  C12Y204/01049

Abstract: The present invention relates to the biocatalytic production of glycosides. In particular, the invention discloses a method to produce alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids via the usage of a cellodextrin phosphorylase (CDP) derived from Clostridium stercorarium (CsCDP). The method of the present invention is feasible for use in industrial glycosylation processes and has advantageous properties compared to classical as chemical glycosylation reactions. Moreover, said method can also be employed to produce corresponding lactosides such a lactolipids when a-galactose-1-phosphate instead of a-glucose-1-phosphate is used as a donor.

Abstract translation: 本发明涉及糖苷的生物催化生产。 特别地，本发明公开了一种通过使用源自灭菌梭菌（CsCDP）的纤维糊酶磷酸化酶（CDP）来生产烷基纤维二糖苷，芳基纤维二糖苷，纤维二糖，纤维三糖，葡萄糖脂和纤维二糖体脂的方法。 本发明的方法可用于工业糖基化方法，并且与经典的化学糖基化反应相比具有有利的性质。 此外，当α-半乳糖-1-磷酸酯代替α-葡萄糖-1-磷酸作为供体时，所述方法也可用于产生相应的乳糖苷如乳糖。

公开(公告)号：US08889379B2

公开(公告)日：2014-11-18

申请号：US13696782

申请日：2011-05-19

Applicant: Giang Hai Tran ,  Tom Desmet ,  Wim Soetaert

Inventor： Giang Hai Tran ,  Tom Desmet ,  Wim Soetaert

IPC: C12P19/44 ,  C12N9/10 ,  C12P19/18

CPC classification number: C12N9/1051 ,  C12P19/18 ,  C12P19/44 ,  C12Y204/01049

Abstract: An in vitro method to produce a glycoside is described which includes the steps of contacting the cellodextrin phosphorylase from Clostridium stercorarium with alpha-glucose-1-phosphate or alpha-galactose-1-phosphate and an acceptor, and glycosylating the acceptor. The acceptor may be an alkyl beta-glucoside, an aryl beta-glucoside, a glucolipid, an alkyl beta-sophoroside, an aryl beta-sophoroside or a sophorolipid. Alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids are produced when alpha-glucose-1-phosphate is used as donor. Corresponding lactosides are produced when alpha-galactose-1-phosphate is used as donor.

Abstract translation: 描述了产生糖苷的体外方法，其包括使来自灭菌梭菌的纤维糊酶磷酸化酶与α-葡萄糖-1-磷酸或α-半乳糖-1-磷酸和受体接触并使受体糖基化的步骤。 受体可以是β-葡萄糖苷基，芳基β-葡糖苷，葡萄糖脂，烷基β-槐糖苷，芳基β-槐糖苷或槐糖脂。 当使用α-葡萄糖-1-磷酸作为供体时，产生烷基纤维二糖苷，芳基纤维二糖苷，纤维二糖，纤维三糖，葡萄糖脂和纤维二糖脂。 当α-半乳糖-1-磷酸被用作供体时，产生相应的乳糖苷。