公开(公告)号：US11932846B2

公开(公告)日：2024-03-19

申请号：US16634898

申请日：2018-08-08

申请人： Technology Innovation Momentum Fund (Israel) Limited Partnership

发明人： Gali Prag

IPC分类号： C12N15/10 ,  C12N15/03 ,  C12Q1/02 ,  C12Q1/48

CPC分类号： C12N15/1055 ,  C12N15/03 ,  C12Q1/025 ,  C12Q1/48 ,  C12Y203/01028 ,  G01N2333/91062 ,  G01N2500/02

摘要： A system for expressing a chloramphenicol split protein is disclosed. Uses thereof are also disclosed.

公开(公告)号：US11891598B2

公开(公告)日：2024-02-06

申请号：US16632998

申请日：2018-07-26

申请人： MEDIZINISCHE HOCHSCHULE HANNOVER (MHH)

发明人： Timm Fiebig ,  Christa Litschko ,  Rita Gerardy-Schahn ,  Andrea Bethe ,  Monika Berger

IPC分类号： C12N15/03 ,  A61K39/02 ,  C12N15/113 ,  A61K39/00

CPC分类号： C12N15/03 ,  A61K39/02 ,  C12N15/113 ,  A61K2039/55583 ,  C12N2310/3517

摘要： The present invention relates to a host cell, which comprises under the control of a heterologous promoter a polynucleotide comprising a nucleotide sequence encoding a polypeptide capable of synthesizing a polysaccharide consisting of a dimeric repeating unit as well as to a vaccine composition comprising such host cell. Furthermore, either such host cell or a polypeptide expressed by such host cell is used for the production of a polysaccharide consisting of a dimeric repeating unit which may be used as a glycoconjugate vaccine.

公开(公告)号：US10662446B2

公开(公告)日：2020-05-26

申请号：US15762332

申请日：2016-09-29

申请人： THE UNIVERSITY OF QUEENSLAND

发明人： Carlos H. Luna-Flores ,  Lars K. Nielsen ,  Esteban Marcellin ,  Christopher C. Stowers ,  Bianca F. Martins ,  Brad M. Cox

IPC分类号： C12N1/21 ,  C12P7/52 ,  C07K14/195 ,  C12N15/03 ,  C12R1/01 ,  C12N9/00 ,  C12N9/10

摘要： Disclosed herein are new strains of Propionibacterium and methods for the biosynthetic production of propionic acid.

公开(公告)号：US10450574B2

公开(公告)日：2019-10-22

申请号：US15356813

申请日：2016-11-21

申请人： GLAXOSMITHKLINE INTELLECTUAL PROPERTY DEVELOPMENT LIMITED

发明人： Sabine Johnson ,  Celeste Pallant ,  Eirini Vamva ,  Conrad Vink

IPC分类号： C12Q1/68 ,  A61K39/21 ,  C12N15/03 ,  C12N15/87 ,  C12N15/867 ,  C12N15/64 ,  C12N7/00 ,  C12N15/85 ,  C12N15/86 ,  C12N15/67 ,  C12N15/88 ,  C12N15/89

摘要： The invention relates to bacterial artificial chromosomes (BAC) comprising retroviral nucleic acid sequences encoding: gag and pol proteins, and an env protein or a functional substitute thereof, wherein each of the retroviral nucleic acid sequences are arranged as individual expression constructs within the BAC. The invention also relates to uses and methods of transient transfection using said BAC.

公开(公告)号：US20190263871A1

公开(公告)日：2019-08-29

申请号：US16307783

申请日：2017-07-20

申请人： Jason DEXTER

发明人： Jason DEXTER

IPC分类号： C07K14/195 ,  C12N1/12 ,  C12N9/50 ,  C12N15/52 ,  C12N1/20 ,  C12N15/03 ,  C12N15/82

摘要： The invention comprises a photoautotrophic organism, generally having simpler nutritional requirements than heterotrophic organisms, utilized as a chassis for the heterologous expression and function of enzymes, or derivatives of said enzymes, that show activity toward the degradation/detoxification of toxins known to be associated with and specific to harmful algal blooms. As an example, a cyanobacterial strain (Synechocystis sp. PCC 6803) modified to express Sphingomonas sp. USTB-05 MlrA enzyme functionality, showing the capability of degrading microcystins (results shown here) and nodularins, is presented. Under modelled natural conditions, results indicate that heterologous enzymatic activity against microcystin-LR is more stable over time when utilizing a photoautotrophic chassis in comparison to use of a heterotrophic bacterial strain. In addition, both the viability and cell density of the photoautotrophic host is maintained for a significantly longer period of time, compared to a heterotrophic host.

公开(公告)号：US10233227B2

公开(公告)日：2019-03-19

申请号：US15105162

申请日：2014-12-19

申请人： Consejo Nacional de Investigaciones Cientificas y Técnicas ,  FUNDACIÓN ARTICULAR ,  INIS BIOTECH LLC

发明人： Ricardo Alfredo Dewey ,  Benito Jorge Velasco Zamora ,  Tania Melina Rodríguez ,  Alejandra Carrea ,  Andrea Nancy Chisari ,  Marcelo Javier Perone

IPC分类号： C12N15/12 ,  C12N15/06 ,  C12N15/03 ,  C12N5/07 ,  C12N5/10 ,  C12N1/21 ,  C07K14/71 ,  C07K16/28 ,  A61K38/00

摘要： An isoform of the TGF beta receptor II comprising a sequence of about of 80 amino acids and lacking a transmembrane domain; wherein the isoform is a TGFβ-1 agonist. The isoform comprises the amino acid sequence set forth in SEQ ID No. 12. The isoform may have the amino acid sequence set forth in SEQ ID No. 2 or sequences having at least 85% sequence identity to the sequence set forth in SEQ ID No. 2.

公开(公告)号：US20170362602A1

公开(公告)日：2017-12-21

申请号：US15624495

申请日：2017-06-15

申请人： University of North Texas ,  The United States of America as represented by the Secretary of Agriculture

发明人： Kent Chapman ,  Robert Mullen ,  Michal Pyc ,  John M. Dyer

IPC分类号： C12N15/82 ,  A01H5/10 ,  C12N15/03 ,  C12Q1/68

CPC分类号： C12N15/8247 ,  A01H5/10 ,  C07K14/415 ,  C12N15/03 ,  C12N15/8201 ,  C12Q1/6895

摘要： The present disclosure relates to increasing oil content in plants through manipulating expression of proteins, namely At5g16550 proteins. At5g16550 proteins are lipid droplet proteins. Plants having reduced expression of At5g16550 proteins demonstrate cytoplasmic lipid droplets (LDs) that are increased in size, resulting in greater overall oil content in the plant cells.

公开(公告)号：US09133483B2

公开(公告)日：2015-09-15

申请号：US14073979

申请日：2013-11-07

申请人： The Trustees of the University of Pennsylvania

发明人： James M Wilson ,  Guangping Gao ,  Soumitra Roy

IPC分类号： C12N15/861 ,  C12N7/01 ,  C12N15/03 ,  C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  A61K39/00 ,  A61K48/00

CPC分类号： A61K39/235 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/525 ,  A61K2039/5256 ,  A61K2039/57 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10022 ,  C12N2710/10321 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10362 ,  C12N2710/20022 ,  C12N2740/16122 ,  C12N2740/17022 ,  C12N2750/14122 ,  C12N2760/14134 ,  C12N2760/16134 ,  C12N2760/20122 ,  C12N2830/002 ,  C12N2830/55

摘要： A recombinant vector comprises simian adenovirus sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.

摘要翻译： 重组载体包含猿猴腺病毒序列和在调节序列控制下的异源基因。 还公开了表达猿腺病毒基因的细胞系。 提供了使用载体和细胞系的方法。

公开(公告)号：US07622297B2

公开(公告)日：2009-11-24

申请号：US11896977

申请日：2007-09-07

申请人： Yasuhiro Yasutomi ,  Hitoshi Mizutani

发明人： Yasuhiro Yasutomi ,  Hitoshi Mizutani

IPC分类号： C12N15/03 ,  C12N15/10 ,  A61K31/711 ,  C07H21/04

CPC分类号： A61K39/04 ,  A61K2039/53

摘要： The α antigen-encoding gene and the α antigen protein suppress the production of interleukin-4 etc., improve the Th2 type cytokine-dominant state, and furthermore inhibit various conditions of allergic diseases such as IgE production, histamine release and eosinophil infiltration, and therefore they are very effective for the prevention or treatment of atopic diseases such as atopic dermatitis, asthma, allergic rhinitis, and allergic conjunctivitis, and more broadly allergic diseases.

摘要翻译： α抗原编码基因和α抗原蛋白抑制白细胞介素-4等的产生，改善Th2型细胞因子显性状态，进一步抑制IgE产生，组胺释放和嗜酸性粒细胞浸润等过敏性疾病的各种病症， 因此它们对预防或治疗特应性皮炎，哮喘，过敏性鼻炎和过敏性结膜炎等特应性疾病以及更广泛的过敏性疾病非常有效。

公开(公告)号：US5840530A

公开(公告)日：1998-11-24

申请号：US685118

申请日：1996-07-23

申请人： Ulrich Andreas Gubler ,  David Howard Presky

发明人： Ulrich Andreas Gubler ,  David Howard Presky

IPC分类号： G01N33/53 ,  A61K31/00 ,  A61K38/00 ,  A61K39/395 ,  A61P37/00 ,  A61P37/02 ,  A61P37/04 ,  C07H21/04 ,  C07K14/52 ,  C07K14/54 ,  C07K14/705 ,  C07K14/715 ,  C07K16/00 ,  C07K16/28 ,  C07K19/00 ,  C12N5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12R1/91 ,  G01N33/566 ,  C12N15/03 ,  C12N15/11

CPC分类号： C07K16/2866 ,  C07K14/7155 ,  A61K38/00 ,  Y10S930/12

摘要： A recombinant human IL-12 beta2 receptor protein produced on the surface of a non-human mammalian cell, free from other human proteins, in its active form. In addition, a non-human mammalian cell having expressed on its surface the recombinant human IL-12 beta2 receptor protein, which cell proliferates in the presence of human IL-12. A non-human mammalian cell having the human IL-12 beta2 receptor protein on its surface and which proliferates in response to human IL-12 is useful for determining whether a given compound inhibits biological activity of human IL-12 or is an IL-12 agonist.

摘要翻译： 在非活性形式的非人哺乳动物细胞表面上产生的，不含其他人类蛋白质的重组人IL-12β2受体蛋白质。 另外，在其表面上表达了人IL-12的重组人IL-12β2受体蛋白的非人哺乳动物细胞，该细胞在人IL-12存在下增殖。 在其表面上具有人IL-12β2受体蛋白的非人哺乳动物细胞，其响应于人IL-12增殖是有用的，用于确定给定的化合物是否抑制人IL-12的生物活性或是IL-12 激动剂。