公开(公告)号：US20190263871A1

公开(公告)日：2019-08-29

申请号：US16307783

申请日：2017-07-20

申请人： Jason DEXTER

发明人： Jason DEXTER

IPC分类号： C07K14/195 ,  C12N1/12 ,  C12N9/50 ,  C12N15/52 ,  C12N1/20 ,  C12N15/03 ,  C12N15/82

摘要： The invention comprises a photoautotrophic organism, generally having simpler nutritional requirements than heterotrophic organisms, utilized as a chassis for the heterologous expression and function of enzymes, or derivatives of said enzymes, that show activity toward the degradation/detoxification of toxins known to be associated with and specific to harmful algal blooms. As an example, a cyanobacterial strain (Synechocystis sp. PCC 6803) modified to express Sphingomonas sp. USTB-05 MlrA enzyme functionality, showing the capability of degrading microcystins (results shown here) and nodularins, is presented. Under modelled natural conditions, results indicate that heterologous enzymatic activity against microcystin-LR is more stable over time when utilizing a photoautotrophic chassis in comparison to use of a heterotrophic bacterial strain. In addition, both the viability and cell density of the photoautotrophic host is maintained for a significantly longer period of time, compared to a heterotrophic host.