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    Pullulanase Variants and Polynucleotides Encoding Same
    2.
    发明申请
    Pullulanase Variants and Polynucleotides Encoding Same 审中-公开

    公开(公告)号：US20200248157A1

    公开(公告)日：2020-08-06

    申请号：US16837166

    申请日：2020-04-01

    申请人： Novozymes A/S

    发明人： Tomoko Matsui ,  Akihiko YAMAGISHI

    IPC分类号： C12N9/44 ,  C12P7/06 ,  C12P19/02 ,  C12P19/16

    摘要： The present invention relates to pullulanase variants comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

    Yield Improvement by PH-Stabilization of Enzymes
    5.
    发明申请
    Yield Improvement by PH-Stabilization of Enzymes 审中-公开
    通过酶的稳定化产生的产量提高

    公开(公告)号：US20160319264A1

    公开(公告)日：2016-11-03

    申请号：US15108344

    申请日：2015-01-08

    申请人： NOVOZYMES A/S

    发明人： Tomoko Matsui

    IPC分类号： C12N9/44 ,  C12Q1/40 ,  C12N9/96

    CPC分类号： C12N9/2457 ,  C12N9/00 ,  C12N9/96 ,  C12Q1/40 ,  C12Y302/01041 ,  G01N2333/944

    摘要： The invention relates to methods of improving the yield or productivity of a variant enzyme derived from a parent enzyme, said method comprising the step of selecting a host cell that produces a variant enzyme which has at least the specific enzymatic activity of the parent enzyme as well as an improved pH-stability in the pH range of 5-9.

    摘要翻译： 本发明涉及提高衍生自亲本酶的变体酶的产量或生产率的方法，所述方法包括选择产生至少具有亲本酶的特异性酶活性的变体酶的宿主细胞的步骤 作为在5-9的pH范围内改善的pH稳定性。

    Pullulanase variants and polynucleotides encoding same
    10.
    发明授权
    Pullulanase variants and polynucleotides encoding same 有权

    公开(公告)号：US11136568B2

    公开(公告)日：2021-10-05

    申请号：US16837166

    申请日：2020-04-01

    申请人： Novozymes A/S

    发明人： Tomoko Matsui ,  Akihiko Yamagishi

    IPC分类号： C12N9/44 ,  C12P19/16 ,  C12P19/02 ,  C12P7/06

    摘要： The present invention relates to pullulanase variants comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.