Provided are methods, compositions, and systems for targeted pseudouridylation of RNA. In some aspects, provided are methods for editing a target RNA (e.g., mRNA) in a host cell, comprising introducing an engineered guide small nucleolar RNA (gsnoRNA) into the host cell, wherein the gsnoRNA recruits a DKC1 protein to modify a target uridine residue into a pseudouridine residue in the target RNA. In some embodiments, the DKC1 protein has cytoplasmic localization in the host cell.