公开(公告)号：US12031165B2

公开(公告)日：2024-07-09

申请号：US17477668

申请日：2021-09-17

申请人： Jiangnan University

发明人： Zhemin Zhou ,  Zhongmei Liu ,  Yufeng Liu ,  Li Zhou ,  Wenjing Cui ,  Junling Guo

IPC分类号： C12P13/04 ,  C12N9/88 ,  C12N9/90

CPC分类号： C12N9/88 ,  C12N9/90 ,  C12P13/04 ,  C12Y401/01012 ,  C12Y504/03009

摘要： Disclosed is a double enzyme tandem preparation method of L-2-aminobutyric acid, and belongs to the field of bioengineering. In the disclosure, recombinant Escherichia coli expressing L-glutamate mutase and recombinant Escherichia coli expressing L-aspartate-β-decarboxylase are separately cultured to obtain L-glutamate mutase and L-aspartate-β-decarboxylase. The two enzymes are added to a reaction system at a certain mass ratio, and L-glutamate is used as a substrate to carry out an enzyme reaction to prepare the L-2-aminobutyric acid. When the dosage of the L-aspartate-β-decarboxylase is 2 mg/mL, and the reaction time is 24 h, 8.5 mmol/L L-2-aminobutyric acid is produced by conversion, with a molar conversion rate of 85.00%. Compared with a chemical production method, the method disclosed by the disclosure has a safe production process and no environmental pollution. Compared with a multi-enzyme synthesis system with threonine as a substrate, the substrate is cheaper and the process is simpler.