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1.发明申请公开(公告)号：US20180080076A1
公开(公告)日：2018-03-22
申请号：US15814761
申请日：2017-11-16
Applicant: The University of Tokyo , Nikon Corporaton
Inventor： Taro Ueno , Takashi Funatsu , Takanori Ichiki , Hirofumi Shiono
IPC: C12Q1/68
CPC classification number: C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/178 , C12Q2521/501 , C12Q2525/301 , C12Q2565/519
Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5′ protruding end or a 3′ protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.
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公开(公告)号：US10294472B2
公开(公告)日：2019-05-21
申请号：US14849992
申请日：2015-09-10
Applicant: The University of Tokyo , Nikon Corporation
Inventor： Takanori Ichiki , Shingo Ueno , Manish Biyani , Ryo Kobayashi , Hirofumi Shiono
IPC: C12N15/10 , C12Q1/6853
Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.
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公开(公告)号：US10113948B2
公开(公告)日：2018-10-30
申请号：US15490727
申请日：2017-04-18
Applicant: The University of Tokyo , NIKON CORPORATION
Inventor： Takanori Ichiki , Kuno Suzuki , Daishi Tanaka , Jiro Inoue , Kazuya Ota
Abstract: A particle detection method in which particles in a sample are detected includes: a mounting step of mounting, on a stage portion, a fluid device including a channel through which the particles can move; an irradiation step of irradiating the channel with illumination light; and a detection step of detecting scattered light generated from the particles by irradiation with the illumination light. In the irradiation step, the illumination light is converged such as to enter the channel by passing through, among side surfaces of the channel, only the first side surface that faces an illumination light incident direction.
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公开(公告)号：US20180100793A1
公开(公告)日：2018-04-12
申请号：US15786450
申请日：2017-10-17
Applicant: The University of Tokyo , Nikon Corporation
Inventor： Takanori Ichiki , Kuno Suzuki , Daishi Tanaka
CPC classification number: G01N15/14 , G01N15/02 , G01N15/0227 , G01N15/1429 , G01N15/1484 , G01N27/26 , G01N33/53 , G01N2015/0053 , G01N2015/1006 , G01N2015/1075 , G01N2015/1486 , G01N2015/1493
Abstract: A microparticle detection system includes a stage unit including a mounting surface on which a fluid device having a flow path through which a sample containing microparticles is movable is capable of being mounted, an emission unit configured to emit illumination light to the flow path, an imaging unit configured to image scattered light generated from microparticles in the sample when illumination light is emitted, an identification unit configured to identify the microparticles included in the image for each of the microparticles on the basis of the image captured by the imaging unit, a particle size determination unit configured to determine a particle size of the microparticle for each of the microparticles identified by the identification unit, a zeta potential determination unit configured to determine a zeta potential of the microparticle for each of the microparticles identified by the identification unit, and a correlation unit configured to associate the particle size for each of the microparticles determined by the particle size determination unit with the zeta potential for each of the microparticles determined by the zeta potential determination unit for each of the microparticles.
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公开(公告)号：US10301682B2
公开(公告)日：2019-05-28
申请号：US15080266
申请日：2016-03-24
Applicant: The University of Tokyo , Nikon Corporation
Inventor： Takanori Ichiki , Masashi Kobayashi , Ayako Hayashi , Shoichi Tsuchiya , Takanori Akagi , Taro Ueno , Takashi Funatsu , Kenji Miyamoto
IPC: C12Q1/6886 , G01N33/53 , B01L3/00 , C12Q1/6806
Abstract: The present invention provides a fluidic device, an exosome analysis method, a biomolecule analysis method, and a biomolecule detection method, which can analyze even the content of an exosome in a series of flows by introducing a sample into the device. A fluidic device of the present invention is a fluidic device which detects a biomolecule contained in an exosome in a sample, and includes: an exosome purification unit which has a layer modified with a compound having a hydrophobic chain and a hydrophilic chain; a biomolecule purification unit; a biomolecule detection unit; a first flow path which connects the exosome purification unit to the biomolecule purification unit; and a second flow path which connects the biomolecule purification unit to the biomolecule detection unit.
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Patent Agency Ranking
公开(公告)号：US09844764B2
公开(公告)日：2017-12-19
申请号：US13772835
申请日：2013-02-21
Applicant: THE UNIVERSITY OF TOKYO , NIKON CORPORATION
Inventor： Takanori Ichiki , Manish Biyani , Hirofumi Shiono
CPC classification number: B01J19/0046 , C07K17/14 , C07K2319/21 , C12P21/02 , C40B20/02 , G01N33/6803
Abstract: The present invention relates to a protein or peptide printing method, comprising (a) a step for preparing nucleic acids and a cell-free protein synthesis system in an engraved plate composed of microscopic grooves having a specific opening shape, (b) a step for superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and (c) a step for synthesizing the protein or peptide from the nucleic acids using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate along the specific opening shapes of the microscopic grooves.
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公开(公告)号：US20160076022A1
公开(公告)日：2016-03-17
申请号：US14849992
申请日：2015-09-10
Applicant: The University of Tokyo , Nikon Corporation
Inventor： Takanori Ichiki , Shingo Ueno , Manish Biyani , Ryo Kobayashi , Hirofumi Shiono
IPC: C12N15/10
CPC classification number: C12N15/1062 , C12N15/1093 , C12Q1/6853 , C12Q2521/107 , C12Q2565/537
Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.
Abstract translation: 本发明涉及用于产生mRNA复合物的核酸连接体和由mRNA编码的蛋白质或肽，所述连接体包含：5'末端的间隔部分; 可与mRNA序列的至少一部分杂交的多核苷酸部分; 以及臂部，其具有在3'末端的蛋白质或肽的连接部分，其中间隔部分，多核苷酸部分和臂部分形成单链，并且其中多核苷酸部分含有光反应性 基础衍生物。
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8.发明申请公开(公告)号：US20140296111A1
公开(公告)日：2014-10-02
申请号：US14266185
申请日：2014-04-30
Applicant: The University of Tokyo , Nikon Corporation
Inventor： Shingo Ueno , Naoto Nemoto , Takanori Ichiki , Hirofumi Shiono , Hisao Osawa
IPC: C12N15/10
CPC classification number: C12N15/1062 , C07K17/14 , C12N15/101 , C12Q1/6844 , C12Q2521/107 , C12Q2523/319 , C12Q2565/537
Abstract: A protein-immobilizing solid phase is a protein-immobilizing solid phase comprising an mRNA-nucleic acid linker-protein complex, obtained by linking the mRNA and the protein encoded by that mRNA through the nucleic acid linker, immobilized on the solid phase, wherein the nucleic acid linker has a photocleavage site and a solid phase binding site.
Abstract translation: 固定蛋白质的固相是固定蛋白的固相，其包含mRNA-核酸接头 - 蛋白复合物，其通过将mRNA和由该mRNA编码的蛋白质通过固定在固相上的核酸接头连接获得，其中 核酸接头具有光切割位点和固相结合位点。
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公开(公告)号：US10385305B2
公开(公告)日：2019-08-20
申请号：US14642265
申请日：2015-03-09
Applicant: THE UNIVERSITY OF TOKYO , NIKON CORPORATION
Inventor： Takanori Ichiki , Hirofumi Shiono
Abstract: A device for measuring activity of cultured cells includes a position detecting unit specifying a position of a cell to be measured, a microchamber controlling unit disposing in the culture container a microchamber which surrounds the cell and forms a measurement space, the measurement space being minute with respect to a volume of the culture container, and a measuring unit measuring environmental factors contained in the measurement space.
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公开(公告)号：US09952215B2
公开(公告)日：2018-04-24
申请号：US14629028
申请日：2015-02-23
Applicant: The University of Tokyo , NIKON CORPORATION
Inventor： Takanori Ichiki , Takanori Akagi , Hirofumi Shiono
IPC: G01N27/447 , G01N33/566 , G01N33/574 , G01N33/543 , G01N33/561
CPC classification number: G01N33/566 , G01N27/44752 , G01N27/44791 , G01N33/5432 , G01N33/54346 , G01N33/561 , G01N33/57484 , G01N2333/70596 , G01N2550/00
Abstract: A method of analyzing an exosome is provided for detecting abnormality in a cell, including: (a) a step of preparing an exosome from a sample; (b) a step of bringing the exosome prepared in the step (a) into contact with a first antibody to a protein which exists on the surface of the exosome as an antigen and forming a first antibody-exosome complex; and (c) a step of measuring a zeta potential of the first antibody-exosome complex.
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