公开(公告)号：US20180080076A1

公开(公告)日：2018-03-22

申请号：US15814761

申请日：2017-11-16

Applicant: The University of Tokyo ,  Nikon Corporaton

Inventor： Taro Ueno ,  Takashi Funatsu ,  Takanori Ichiki ,  Hirofumi Shiono

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2600/178 ,  C12Q2521/501 ,  C12Q2525/301 ,  C12Q2565/519

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5′ protruding end or a 3′ protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.

公开(公告)号：US10385305B2

公开(公告)日：2019-08-20

申请号：US14642265

申请日：2015-03-09

Applicant: THE UNIVERSITY OF TOKYO ,  NIKON CORPORATION

Inventor： Takanori Ichiki ,  Hirofumi Shiono

IPC: C12M1/34 ,  C12Q1/02 ,  G01N33/50

Abstract: A device for measuring activity of cultured cells includes a position detecting unit specifying a position of a cell to be measured, a microchamber controlling unit disposing in the culture container a microchamber which surrounds the cell and forms a measurement space, the measurement space being minute with respect to a volume of the culture container, and a measuring unit measuring environmental factors contained in the measurement space.

公开(公告)号：US09952215B2

公开(公告)日：2018-04-24

申请号：US14629028

申请日：2015-02-23

Applicant: The University of Tokyo ,  NIKON CORPORATION

Inventor： Takanori Ichiki ,  Takanori Akagi ,  Hirofumi Shiono

IPC: G01N27/447 ,  G01N33/566 ,  G01N33/574 ,  G01N33/543 ,  G01N33/561

CPC classification number: G01N33/566 ,  G01N27/44752 ,  G01N27/44791 ,  G01N33/5432 ,  G01N33/54346 ,  G01N33/561 ,  G01N33/57484 ,  G01N2333/70596 ,  G01N2550/00

Abstract: A method of analyzing an exosome is provided for detecting abnormality in a cell, including: (a) a step of preparing an exosome from a sample; (b) a step of bringing the exosome prepared in the step (a) into contact with a first antibody to a protein which exists on the surface of the exosome as an antigen and forming a first antibody-exosome complex; and (c) a step of measuring a zeta potential of the first antibody-exosome complex.

公开(公告)号：US20150051105A1

公开(公告)日：2015-02-19

申请号：US14510282

申请日：2014-10-09

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Taro Ueno ,  Takashi Funatsu ,  Takanori Ichiki ,  Hirofumi Shiono

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2600/178 ,  C12Q2521/501 ,  C12Q2525/301 ,  C12Q2565/519

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5′ protruding end or a 3′ protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.

Abstract translation: 一种检测靶核酸的方法，包括：（a）将包含第一部分和第二部分的靶核酸的核酸样品与以下物质接触：（i）检测探针，其中所述检测探针用 标记物质，并且包含形成茎 - 环结构并具有能够与第二部分杂交的5'突出端或3'突出端的核酸序列，和（ii）包含核酸的捕获探针 能够与第一部分杂交的序列，其中捕获探针固定在底物上，条件是通过将第二部分与检测探针杂交形成靶核酸检测探针 - 捕获探针复合体，并将第一部分与 捕获探针 （b）将检测探针的第一端与靶核酸的末端连接，并将检测探针的第二末端与捕获探针的末端连接; 和（c）检测形成在基板上的核酸检测探针 - 捕获探针复合体的标记物质。

公开(公告)号：US09850535B2

公开(公告)日：2017-12-26

申请号：US14510282

申请日：2014-10-09

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Taro Ueno ,  Takashi Funatsu ,  Takanori Ichiki ,  Hirofumi Shiono

IPC: C12Q1/68 ,  C07H21/00 ,  C12P19/34

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2600/178 ,  C12Q2521/501 ,  C12Q2525/301 ,  C12Q2565/519

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5′ protruding end or a 3′ protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.

公开(公告)号：US20150168400A1

公开(公告)日：2015-06-18

申请号：US14629028

申请日：2015-02-23

Applicant: The University of Tokyo ,  NIKON CORPORATION

Inventor： Takanori ICHIKI ,  Takanori Akagi ,  Hirofumi Shiono

IPC: G01N33/566 ,  G01N27/447

CPC classification number: G01N33/566 ,  G01N27/44752 ,  G01N27/44791 ,  G01N33/5432 ,  G01N33/54346 ,  G01N33/561 ,  G01N33/57484 ,  G01N2333/70596 ,  G01N2550/00

Abstract: A method of analyzing an exosome is provided for detecting abnormality in a cell, including: (a) a step of preparing an exosome from a sample; (b) a step of bringing the exosome prepared in the step (a) into contact with a first antibody to a protein which exists on the surface of the exosome as an antigen and forming a first antibody-exosome complex; and (c) a step of measuring a zeta potential of the first antibody-exosome complex.

Abstract translation: 提供分析外来体的方法，用于检测细胞异常，包括：（a）从样品制备外来体的步骤; （b）使步骤（a）中制备的外来体与作为抗原存在于外来体表面上的蛋白质的第一抗体接触并形成第一抗体 - 外来体复合物的步骤; 和（c）测量第一抗体 - 外来体复合物的ζ电位的步骤。

公开(公告)号：US09844764B2

公开(公告)日：2017-12-19

申请号：US13772835

申请日：2013-02-21

Applicant: THE UNIVERSITY OF TOKYO ,  NIKON CORPORATION

Inventor： Takanori Ichiki ,  Manish Biyani ,  Hirofumi Shiono

IPC: B01J19/00 ,  G01N33/68 ,  C07K17/14 ,  C12P21/02 ,  C40B20/02

CPC classification number: B01J19/0046 ,  C07K17/14 ,  C07K2319/21 ,  C12P21/02 ,  C40B20/02 ,  G01N33/6803

Abstract: The present invention relates to a protein or peptide printing method, comprising (a) a step for preparing nucleic acids and a cell-free protein synthesis system in an engraved plate composed of microscopic grooves having a specific opening shape, (b) a step for superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and (c) a step for synthesizing the protein or peptide from the nucleic acids using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate along the specific opening shapes of the microscopic grooves.

公开(公告)号：US20160076022A1

公开(公告)日：2016-03-17

申请号：US14849992

申请日：2015-09-10

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Takanori Ichiki ,  Shingo Ueno ,  Manish Biyani ,  Ryo Kobayashi ,  Hirofumi Shiono

IPC: C12N15/10

CPC classification number: C12N15/1062 ,  C12N15/1093 ,  C12Q1/6853 ,  C12Q2521/107 ,  C12Q2565/537

Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

Abstract translation: 本发明涉及用于产生mRNA复合物的核酸连接体和由mRNA编码的蛋白质或肽，所述连接体包含：5'末端的间隔部分; 可与mRNA序列的至少一部分杂交的多核苷酸部分; 以及臂部，其具有在3'末端的蛋白质或肽的连接部分，其中间隔部分，多核苷酸部分和臂部分形成单链，并且其中多核苷酸部分含有光反应性 基础衍生物。

公开(公告)号：US20140296111A1

公开(公告)日：2014-10-02

申请号：US14266185

申请日：2014-04-30

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Shingo Ueno ,  Naoto Nemoto ,  Takanori Ichiki ,  Hirofumi Shiono ,  Hisao Osawa

IPC: C12N15/10

CPC classification number: C12N15/1062 ,  C07K17/14 ,  C12N15/101 ,  C12Q1/6844 ,  C12Q2521/107 ,  C12Q2523/319 ,  C12Q2565/537

Abstract: A protein-immobilizing solid phase is a protein-immobilizing solid phase comprising an mRNA-nucleic acid linker-protein complex, obtained by linking the mRNA and the protein encoded by that mRNA through the nucleic acid linker, immobilized on the solid phase, wherein the nucleic acid linker has a photocleavage site and a solid phase binding site.

Abstract translation: 固定蛋白质的固相是固定蛋白的固相，其包含mRNA-核酸接头 - 蛋白复合物，其通过将mRNA和由该mRNA编码的蛋白质通过固定在固相上的核酸接头连接获得，其中 核酸接头具有光切割位点和固相结合位点。

公开(公告)号：US10294472B2

公开(公告)日：2019-05-21

申请号：US14849992

申请日：2015-09-10

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Takanori Ichiki ,  Shingo Ueno ,  Manish Biyani ,  Ryo Kobayashi ,  Hirofumi Shiono

IPC: C12N15/10 ,  C12Q1/6853

Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.