公开(公告)号：US10301682B2

公开(公告)日：2019-05-28

申请号：US15080266

申请日：2016-03-24

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Takanori Ichiki ,  Masashi Kobayashi ,  Ayako Hayashi ,  Shoichi Tsuchiya ,  Takanori Akagi ,  Taro Ueno ,  Takashi Funatsu ,  Kenji Miyamoto

IPC: C12Q1/6886 ,  G01N33/53 ,  B01L3/00 ,  C12Q1/6806

Abstract: The present invention provides a fluidic device, an exosome analysis method, a biomolecule analysis method, and a biomolecule detection method, which can analyze even the content of an exosome in a series of flows by introducing a sample into the device. A fluidic device of the present invention is a fluidic device which detects a biomolecule contained in an exosome in a sample, and includes: an exosome purification unit which has a layer modified with a compound having a hydrophobic chain and a hydrophilic chain; a biomolecule purification unit; a biomolecule detection unit; a first flow path which connects the exosome purification unit to the biomolecule purification unit; and a second flow path which connects the biomolecule purification unit to the biomolecule detection unit.

公开(公告)号：US09850535B2

公开(公告)日：2017-12-26

申请号：US14510282

申请日：2014-10-09

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Taro Ueno ,  Takashi Funatsu ,  Takanori Ichiki ,  Hirofumi Shiono

IPC: C12Q1/68 ,  C07H21/00 ,  C12P19/34

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2600/178 ,  C12Q2521/501 ,  C12Q2525/301 ,  C12Q2565/519

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5′ protruding end or a 3′ protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.

公开(公告)号：US20150051105A1

公开(公告)日：2015-02-19

申请号：US14510282

申请日：2014-10-09

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Taro Ueno ,  Takashi Funatsu ,  Takanori Ichiki ,  Hirofumi Shiono

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2600/178 ,  C12Q2521/501 ,  C12Q2525/301 ,  C12Q2565/519

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5′ protruding end or a 3′ protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.

Abstract translation: 一种检测靶核酸的方法，包括：（a）将包含第一部分和第二部分的靶核酸的核酸样品与以下物质接触：（i）检测探针，其中所述检测探针用 标记物质，并且包含形成茎 - 环结构并具有能够与第二部分杂交的5'突出端或3'突出端的核酸序列，和（ii）包含核酸的捕获探针 能够与第一部分杂交的序列，其中捕获探针固定在底物上，条件是通过将第二部分与检测探针杂交形成靶核酸检测探针 - 捕获探针复合体，并将第一部分与 捕获探针 （b）将检测探针的第一端与靶核酸的末端连接，并将检测探针的第二末端与捕获探针的末端连接; 和（c）检测形成在基板上的核酸检测探针 - 捕获探针复合体的标记物质。

公开(公告)号：US20180080076A1

公开(公告)日：2018-03-22

申请号：US15814761

申请日：2017-11-16

Applicant: The University of Tokyo ,  Nikon Corporaton

Inventor： Taro Ueno ,  Takashi Funatsu ,  Takanori Ichiki ,  Hirofumi Shiono

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2600/178 ,  C12Q2521/501 ,  C12Q2525/301 ,  C12Q2565/519

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5′ protruding end or a 3′ protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.