公开(公告)号：US20180080076A1

公开(公告)日：2018-03-22

申请号：US15814761

申请日：2017-11-16

Applicant: The University of Tokyo ,  Nikon Corporaton

Inventor： Taro Ueno ,  Takashi Funatsu ,  Takanori Ichiki ,  Hirofumi Shiono

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2600/178 ,  C12Q2521/501 ,  C12Q2525/301 ,  C12Q2565/519

Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5′ protruding end or a 3′ protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.

公开(公告)号：US20220227660A1

公开(公告)日：2022-07-21

申请号：US17665092

申请日：2022-02-04

Applicant: NIKON CORPORATON

Inventor： Kohei YOSHIMOTO

IPC: C03C3/247 ,  G02B1/00 ,  G02C7/08

Abstract: The present disclosure provides an optical glass including: by cation %, 30% to 45% of a P5+ component; 10% to 20% of an Al3+ component; and 20% to 40% of a Ba2+ component, and, by anion %, 75% to 95% of an O2− component; and 5% to 25% of an F− component.