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    NUCLEIC ACID DETECTION METHOD, DETECTION PROBE, DETECTION PROBE SET, AND NUCLEIC ACID QUANTIFICATION METHOD
    3.
    发明申请
    NUCLEIC ACID DETECTION METHOD, DETECTION PROBE, DETECTION PROBE SET, AND NUCLEIC ACID QUANTIFICATION METHOD 有权
    核酸检测方法，检测探针，检测探针组和核酸定量方法

    公开(公告)号：US20150051105A1

    公开(公告)日：2015-02-19

    申请号：US14510282

    申请日：2014-10-09

    Applicant: The University of Tokyo ,  Nikon Corporation

    Inventor： Taro Ueno ,  Takashi Funatsu ,  Takanori Ichiki ,  Hirofumi Shiono

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2600/178 ,  C12Q2521/501 ,  C12Q2525/301 ,  C12Q2565/519

    Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5′ protruding end or a 3′ protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.

    Abstract translation: 一种检测靶核酸的方法，包括：（a）将包含第一部分和第二部分的靶核酸的核酸样品与以下物质接触：（i）检测探针，其中所述检测探针用 标记物质，并且包含形成茎 - 环结构并具有能够与第二部分杂交的5'突出端或3'突出端的核酸序列，和（ii）包含核酸的捕获探针 能够与第一部分杂交的序列，其中捕获探针固定在底物上，条件是通过将第二部分与检测探针杂交形成靶核酸检测探针 - 捕获探针复合体，并将第一部分与 捕获探针 （b）将检测探针的第一端与靶核酸的末端连接，并将检测探针的第二末端与捕获探针的末端连接; 和（c）检测形成在基板上的核酸检测探针 - 捕获探针复合体的标记物质。

    NUCLEIC ACID LINKER
    5.
    发明申请
    NUCLEIC ACID LINKER 审中-公开
    核酸连接器

    公开(公告)号：US20160076022A1

    公开(公告)日：2016-03-17

    申请号：US14849992

    申请日：2015-09-10

    Applicant: The University of Tokyo ,  Nikon Corporation

    Inventor： Takanori Ichiki ,  Shingo Ueno ,  Manish Biyani ,  Ryo Kobayashi ,  Hirofumi Shiono

    IPC: C12N15/10

    CPC classification number: C12N15/1062 ,  C12N15/1093 ,  C12Q1/6853 ,  C12Q2521/107 ,  C12Q2565/537

    Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

    Abstract translation: 本发明涉及用于产生mRNA复合物的核酸连接体和由mRNA编码的蛋白质或肽，所述连接体包含：5'末端的间隔部分; 可与mRNA序列的至少一部分杂交的多核苷酸部分; 以及臂部，其具有在3'末端的蛋白质或肽的连接部分，其中间隔部分，多核苷酸部分和臂部分形成单链，并且其中多核苷酸部分含有光反应性 基础衍生物。

    Nucleic acid linker
    9.
    发明授权
    Nucleic acid linker 有权

    公开(公告)号：US10294472B2

    公开(公告)日：2019-05-21

    申请号：US14849992

    申请日：2015-09-10

    Applicant: The University of Tokyo ,  Nikon Corporation

    Inventor： Takanori Ichiki ,  Shingo Ueno ,  Manish Biyani ,  Ryo Kobayashi ,  Hirofumi Shiono

    IPC: C12N15/10 ,  C12Q1/6853

    Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

    Fluidic device, system, and method
    10.
    发明授权
    Fluidic device, system, and method 有权

    公开(公告)号：US10775368B2

    公开(公告)日：2020-09-15

    申请号：US15679816

    申请日：2017-08-17

    Applicant: The University of Tokyo ,  NIKON CORPORATION

    Inventor： Takanori Ichiki ,  Taro Ueno ,  Ryo Kobayashi ,  Hirofumi Shiono

    IPC: G01N33/543 ,  B01F5/10 ,  B01F13/00 ,  B01L3/00 ,  C12Q1/6834

    Abstract: A fluidic device includes: a circulation flow path; and a capture part arranged on the circulation flow path and configured to capture a sample substance in a solution and/or a detection part arranged on the circulation flow path and configured to detect a sample substance in a solution. A method of capturing a sample substance that is bound to a carrier particle, using a fluidic device which includes a circulation flow path and a capture part arranged on the circulation flow path and configured to capture the carrier particle and in which the circulation flow path has two or more circulation flow path valves, includes: an introduction step of, in a state where the circulation flow path valve is closed, introducing a solution that includes a sample substance to at least one of partitions partitioned by the circulation flow path valve and introducing a solution that includes a carrier particle which is bound to the sample substance to at least another of the partitions; a mix step of opening all of the circulation flow path valves and circulating and mixing a solution in the circulation flow path; and a capture step of capturing the carrier particle by the capture part.

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