公开(公告)号：US10294472B2

公开(公告)日：2019-05-21

申请号：US14849992

申请日：2015-09-10

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Takanori Ichiki ,  Shingo Ueno ,  Manish Biyani ,  Ryo Kobayashi ,  Hirofumi Shiono

IPC: C12N15/10 ,  C12Q1/6853

Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

公开(公告)号：US09844764B2

公开(公告)日：2017-12-19

申请号：US13772835

申请日：2013-02-21

Applicant: THE UNIVERSITY OF TOKYO ,  NIKON CORPORATION

Inventor： Takanori Ichiki ,  Manish Biyani ,  Hirofumi Shiono

IPC: B01J19/00 ,  G01N33/68 ,  C07K17/14 ,  C12P21/02 ,  C40B20/02

CPC classification number: B01J19/0046 ,  C07K17/14 ,  C07K2319/21 ,  C12P21/02 ,  C40B20/02 ,  G01N33/6803

Abstract: The present invention relates to a protein or peptide printing method, comprising (a) a step for preparing nucleic acids and a cell-free protein synthesis system in an engraved plate composed of microscopic grooves having a specific opening shape, (b) a step for superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and (c) a step for synthesizing the protein or peptide from the nucleic acids using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate along the specific opening shapes of the microscopic grooves.

公开(公告)号：US20160076022A1

公开(公告)日：2016-03-17

申请号：US14849992

申请日：2015-09-10

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Takanori Ichiki ,  Shingo Ueno ,  Manish Biyani ,  Ryo Kobayashi ,  Hirofumi Shiono

IPC: C12N15/10

CPC classification number: C12N15/1062 ,  C12N15/1093 ,  C12Q1/6853 ,  C12Q2521/107 ,  C12Q2565/537

Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

Abstract translation: 本发明涉及用于产生mRNA复合物的核酸连接体和由mRNA编码的蛋白质或肽，所述连接体包含：5'末端的间隔部分; 可与mRNA序列的至少一部分杂交的多核苷酸部分; 以及臂部，其具有在3'末端的蛋白质或肽的连接部分，其中间隔部分，多核苷酸部分和臂部分形成单链，并且其中多核苷酸部分含有光反应性 基础衍生物。

公开(公告)号：US20130237430A1

公开(公告)日：2013-09-12

申请号：US13772835

申请日：2013-02-21

Applicant: THE UNIVERSITY OF TOKYO

Inventor： Takanori Ichiki ,  Manish Biyani ,  Hirofumi Shiono

IPC: B01J19/00 ,  G01N33/68

CPC classification number: B01J19/0046 ,  C07K17/14 ,  C07K2319/21 ,  C12P21/02 ,  C40B20/02 ,  G01N33/6803

Abstract: The present invention relates to a protein or peptide printing method, comprising (a) a step for preparing nucleic acids and a cell-free protein synthesis system in an engraved plate composed of microscopic grooves having a specific opening shape, (b) a step for superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and (c) a step for synthesizing the protein or peptide from the nucleic acids using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate along the specific opening shapes of the microscopic grooves.

Abstract translation: 本发明涉及一种蛋白质或肽印刷方法，其包括（a）在由具有特定开口形状的微细凹槽构成的雕刻板中制备核酸和无细胞蛋白质合成系统的步骤，（b） 将基底叠加在雕刻板上，以便接触在微细凹槽中合成的蛋白质或肽，和（c）使用无细胞蛋白质合成系统在微观上合成来自核酸的蛋白质或肽的步骤 凹槽，并沿着微细凹槽的特定开口形状将蛋白质或肽固定在基底上。