Invention Grant
- Patent Title: Protein or peptide printing method, protein array or peptide array, and functional protein or functional peptide identification method
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Application No.: US13772835Application Date: 2013-02-21
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Publication No.: US09844764B2Publication Date: 2017-12-19
- Inventor: Takanori Ichiki , Manish Biyani , Hirofumi Shiono
- Applicant: THE UNIVERSITY OF TOKYO , NIKON CORPORATION
- Applicant Address: JP Tokyo JP Tokyo
- Assignee: University of Tokyo,Nikon Corporation
- Current Assignee: University of Tokyo,Nikon Corporation
- Current Assignee Address: JP Tokyo JP Tokyo
- Agency: Finnegan, Henderson, Farabow, Garrett & Dunner, LLP
- Priority: JP2010-191060 20100827; JP2010-258302 20101118
- Main IPC: B01J19/00
- IPC: B01J19/00 ; G01N33/68 ; C07K17/14 ; C12P21/02 ; C40B20/02
Abstract:
The present invention relates to a protein or peptide printing method, comprising (a) a step for preparing nucleic acids and a cell-free protein synthesis system in an engraved plate composed of microscopic grooves having a specific opening shape, (b) a step for superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and (c) a step for synthesizing the protein or peptide from the nucleic acids using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate along the specific opening shapes of the microscopic grooves.
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