公开(公告)号：US20160076022A1

公开(公告)日：2016-03-17

申请号：US14849992

申请日：2015-09-10

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Takanori Ichiki ,  Shingo Ueno ,  Manish Biyani ,  Ryo Kobayashi ,  Hirofumi Shiono

IPC: C12N15/10

CPC classification number: C12N15/1062 ,  C12N15/1093 ,  C12Q1/6853 ,  C12Q2521/107 ,  C12Q2565/537

Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

Abstract translation: 本发明涉及用于产生mRNA复合物的核酸连接体和由mRNA编码的蛋白质或肽，所述连接体包含：5'末端的间隔部分; 可与mRNA序列的至少一部分杂交的多核苷酸部分; 以及臂部，其具有在3'末端的蛋白质或肽的连接部分，其中间隔部分，多核苷酸部分和臂部分形成单链，并且其中多核苷酸部分含有光反应性 基础衍生物。

公开(公告)号：US10775368B2

公开(公告)日：2020-09-15

申请号：US15679816

申请日：2017-08-17

Applicant: The University of Tokyo ,  NIKON CORPORATION

Inventor： Takanori Ichiki ,  Taro Ueno ,  Ryo Kobayashi ,  Hirofumi Shiono

IPC: G01N33/543 ,  B01F5/10 ,  B01F13/00 ,  B01L3/00 ,  C12Q1/6834

Abstract: A fluidic device includes: a circulation flow path; and a capture part arranged on the circulation flow path and configured to capture a sample substance in a solution and/or a detection part arranged on the circulation flow path and configured to detect a sample substance in a solution. A method of capturing a sample substance that is bound to a carrier particle, using a fluidic device which includes a circulation flow path and a capture part arranged on the circulation flow path and configured to capture the carrier particle and in which the circulation flow path has two or more circulation flow path valves, includes: an introduction step of, in a state where the circulation flow path valve is closed, introducing a solution that includes a sample substance to at least one of partitions partitioned by the circulation flow path valve and introducing a solution that includes a carrier particle which is bound to the sample substance to at least another of the partitions; a mix step of opening all of the circulation flow path valves and circulating and mixing a solution in the circulation flow path; and a capture step of capturing the carrier particle by the capture part.

公开(公告)号：US10294472B2

公开(公告)日：2019-05-21

申请号：US14849992

申请日：2015-09-10

Applicant: The University of Tokyo ,  Nikon Corporation

Inventor： Takanori Ichiki ,  Shingo Ueno ,  Manish Biyani ,  Ryo Kobayashi ,  Hirofumi Shiono

IPC: C12N15/10 ,  C12Q1/6853

Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5′-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3′-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

公开(公告)号：US11369959B2

公开(公告)日：2022-06-28

申请号：US16771174

申请日：2017-12-13

Applicant: NIKON CORPORATION

Inventor： Ryo Kobayashi ,  Naoya Ishizawa ,  Taro Ueno

IPC: B01L3/00 ,  G01N35/08

Abstract: The present invention aims at providing a fluidic device that can hold a large amount of solutions in a reservoir without depending on an attitude. The reservoir includes a meandering flow path including: a plurality of first flow paths that extend linearly along a first direction and that are arranged to be spaced in a second direction crossing the first direction; and a second flow path that extends linearly along the second direction such that a connection between first end sides of the adjacent first flow paths and a connection between second end sides of the adjacent first flow paths are alternately switched along the second direction for each first flow path, wherein the meandering flow path meanders along the second direction. When the length of each of the first flow path and the second flow path is L, a surface tension is γ, the density of the solution is ρ, the acceleration which includes a gravity and which is applied to the solution is G, the wetted perimeter length of the first flow path and the second flow path is Wp, a cross-sectional area of the first flow path and the second flow path is A, a receding contact angle is α, and an advancing contact angle is β, a relationship L≤(γ×Wp×(cos α−cos β))/(ρ×A×G) is satisfied.

公开(公告)号：USD933253S1

公开(公告)日：2021-10-12

申请号：US29664120

申请日：2018-09-21

Applicant: Nikon Corporation

Designer： Naoya Ishizawa ,  Taro Ueno ,  Ryo Kobayashi

公开(公告)号：US11511244B2

公开(公告)日：2022-11-29

申请号：US16209848

申请日：2018-12-04

Applicant: NIKON CORPORATION

Inventor： Keiji Mitsui ,  Ryo Kobayashi

IPC: B01F25/51 ,  B01F25/54 ,  C12Q1/68 ,  B03C1/00 ,  B81B7/02 ,  G01N37/00 ,  B81B1/00 ,  C12M1/00 ,  G01N35/08 ,  B01F23/451 ,  B01F35/75 ,  G01N1/38 ,  G01N35/00

Abstract: A fluidic device for capturing or detecting a sample substance contained in a solution includes at least two continuous circulation flow channels selected from the group consisting of: a first type continuous circulation flow channel which is formed of a first circulation flow channel and a second circulation flow channel and which is configured to circulate the solution in the first circulation flow channel and then circulate the solution in the second circulation flow channel; and a second type continuous circulation flow channel which is formed of a third circulation flow channel and a fourth circulation flow channel and which is configured to circulate the solution in the third circulation flow channel and then circulate and mix the solution in both of the third and fourth circulation flow channels, wherein any one of the circulation flow channels has a capturing section which captures the sample substance, and/or a detecting section which detects the sample substance.

公开(公告)号：US11504712B2

公开(公告)日：2022-11-22

申请号：US16772093

申请日：2017-12-28

Applicant: NIKON CORPORATION

Inventor： Naoya Ishizawa ,  Taro Ueno ,  Ryo Kobayashi ,  Tetsuomi Takasaki

IPC: B01L3/00

Abstract: A fluid device includes a substrate and a gas-liquid separating filter, the substrate has a flow path through which a solution flows, a reservoir, in which the solution is accommodated, connected to the flow path, an injection hole configured to connect the reservoir to the outside, and an air introduction hole branched off from the injection hole and connected to the outside, and the gas-liquid separating filter is disposed in a path of the air introduction hole, allows passage of a gas flowing through the air introduction hole, and prevents passage of a liquid flowing through the air introduction hole.

公开(公告)号：US12083518B2

公开(公告)日：2024-09-10

申请号：US17256156

申请日：2018-06-29

Applicant: NIKON CORPORATION

Inventor： Ryo Kobayashi ,  Tetsuomi Takasaki ,  Naoya Ishizawa ,  Taichi Nakamura

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502707 ,  B01L2200/0684 ,  B01L2400/06

Abstract: An object of the present invention is to provide a flow path device capable of suppressing an occurrence of air bubbles when a solution is introduced into a flow path. A fluidic device has a pair of substrates which are stacked in a thickness direction, one substrate including a flow path formed by being covered with the other substrate. The flow path includes a merging/branching portion which is surrounded by a contour which is configured to match each line segment connecting together apex positions of an equilateral triangle as viewed in the thickness direction or a contour parallel to each line segment and in which solution merges or branches. A valve which is configured to regulate flow of a fluid in the flow path is provided in at least two of the apex positions.

公开(公告)号：US11192106B2

公开(公告)日：2021-12-07

申请号：US16209857

申请日：2018-12-04

Applicant: Nikon Corporation

Inventor： Ryo Kobayashi ,  Keiji Mitsui ,  Taro Ueno ,  Hirofumi Shiono

IPC: B01L3/00 ,  G01N1/34 ,  C12Q1/68 ,  B01J19/00 ,  G01N35/08 ,  B81B7/02 ,  C12M1/00 ,  G01N37/00 ,  B81B1/00 ,  G01N1/10 ,  B03C1/00 ,  B03C1/28 ,  C12N15/09 ,  B03C1/01

Abstract: A fluidic device includes a first circulation flow path and a second circulation flow path which circulate a solution containing a sample material, the first circulation flow path and the second circulation flow path share at least a part of the flow path, and at least one selected from the group consisting of a capture unit which captures the sample material, a detection unit which detects the sample material, a valve, and a pump is provided on the shared flow path.

公开(公告)号：US11982602B2

公开(公告)日：2024-05-14

申请号：US17256151

申请日：2018-06-29

Applicant: NIKON CORPORATION

Inventor： Ryo Kobayashi ,  Hirofumi Shiono

IPC: G01N1/38 ,  B01F33/301 ,  G01N35/08

CPC classification number: G01N1/38 ,  B01F33/3017 ,  G01N35/085 ,  G01N2001/383

Abstract: The present invention provides a fluidic device in which solutions of different concentrations can be easily obtained. The fluidic device includes: a first substrate and a second substrate which are stacked in a thickness direction; an undiluted solution introduction flow path which has an undiluted solution introduction port and which is constituted of a groove part provided on at least one of the first substrate and the second substrate; a first circulation flow path which is constituted of a groove part having an annular shape and having a shared part that shares part of a flow path with the undiluted solution introduction flow path and a non-shared part which is not shared with the undiluted solution introduction flow path and which is connected to a diluting solution introduction port; a second circulation flow path which is provided independently of the first circulation flow path and which is constituted of a groove part having an annular shape and having a shared part that shares some flow path with the undiluted solution introduction flow path and a non-shared part which is not shared with the undiluted solution introduction flow path and which is connected to a diluting solution introduction port; and/or a third circulation flow path which is constituted of a groove part having an annular shape and having a shared flow path that shares part of a flow path with the first circulation flow path and a non-shared flow path which is not shared with the first circulation flow path and which is connected to a diluting solution introduction port, wherein the undiluted solution introduction flow path includes a valve at both ends of the shared part.