公开(公告)号：US20230152226A1

公开(公告)日：2023-05-18

申请号：US17949073

申请日：2022-09-20

Applicant: LumiraDx UK Ltd.

Inventor： Stephen V. Fiacco ,  Amanda N. Bruin ,  Matthew J. Willmore ,  Tristin E. Rose ,  Guillermo Martinez-Ariza ,  Keerthi Boddupally

IPC: G01N21/64 ,  B01L3/00

CPC classification number: G01N21/6428 ,  B01L3/502761 ,  G01N2021/6439 ,  B01L2300/0654 ,  B01L2200/16 ,  B01L2200/0652

Abstract: An optical sensor is used to detect a target in a sample liquid. The optical sensor has an optical sensing layer including, in the same layer, a fluorescent reporter, a fluorescent reference, and an optical isolating reagent. The optical sensing layer has an outer surface that contacts the sample liquid and an opposing surface through which a light source irradiates the optical sensing layer with excitation light, and a detector detects fluorescence emitted from within the optical sensing layer. The optical isolating reagent reduces the amount of (i) excitation light that passes through the optical sensing layer and reaches the sample liquid and (ii) background fluorescence that is emitted within the sample liquid and passes back through the optical sensing layer to the detector. Accordingly, the optical reporter and optical reference fluorescence can be detected with higher signal to noise ratios than in the absence of the optical isolating reagent.

公开(公告)号：US20230149928A1

公开(公告)日：2023-05-18

申请号：US17916633

申请日：2021-04-02

Applicant: FemtoDx, Inc.

Inventor： David T. Weaver ,  Robert DiNello ,  Pritiraj Mohanty

IPC: B01L3/00 ,  C12Q1/6844 ,  B01L7/00

CPC classification number: B01L3/502761 ,  C12Q1/6844 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2200/16 ,  B01L2300/0877 ,  B01L2200/0652 ,  B01L2300/18

Abstract: Devices and methods for the detection of nucleic acids (e.g., RNA, DNA) are described. The nucleic acid can be obtained or derived from a pathogen, such as a virus. In one embodiment, the virus is a coronavirus (e.g., SARS-CoV-2) related to the disease COVID-19. Accordingly, devices and methods may be used in the field as point-of-care devices to test a subject (e.g., a patient) for the presence of the SARS-CoV-2 virus or another nucleic acid.

公开(公告)号：US20190234976A1

公开(公告)日：2019-08-01

申请号：US16376380

申请日：2019-04-05

Applicant: Lawrence Livermore National Security, LLC

Inventor： Brian Giera ,  Eric B. Duoss ,  Du Nguyen ,  William Smith ,  Sachin Subhash Talathi ,  Aaron Creighton Wilson ,  Congwang Ye

IPC: G01N35/00 ,  G05B13/02 ,  G05D7/06 ,  B01L3/00 ,  G01N15/00

CPC classification number: G01N35/00871 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/143 ,  G01N15/00 ,  G01N15/1429 ,  G01N15/1475 ,  G01N35/00623 ,  G01N2015/149 ,  G01N2035/00643 ,  G01N2035/00881 ,  G05B13/027 ,  G05D7/0635 ,  G05D7/0694

Abstract: A system is provided to automatically monitor and control the operation of a microfluidic device using machine learning technology. The system receives images of a channel of a microfluidic device collected by a camera during operation of the microfluidic device. Upon receiving an image, the system applies a classifier to the image to classify the operation of the microfluidic device as normal, in which no adjustment to the operation is needed, or as abnormal, in which an adjustment to the operation is needed. When an image is classified as normal, the system may make no adjustment to the microfluidic device. If, however, an image is classified as abnormal, the system may output an indication that the operation is abnormal, output an indication of a needed adjustment, or control the microfluidic device to make the needed adjustment.

公开(公告)号：US20190232290A1

公开(公告)日：2019-08-01

申请号：US16095107

申请日：2017-04-20

Applicant: Cellix Limited

Inventor： Dmitry KASHANIN ,  Igor SHVETS ,  Francesco DICORATO

IPC: B01L3/00 ,  C12M1/00 ,  G01N15/14

CPC classification number: B01L3/502776 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0861 ,  B01L2400/0463 ,  B01L2400/0469 ,  C12M47/04 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/0038 ,  G01N2015/0053 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1413

Abstract: A microfluidic chip for focussing a stream of particulate containing fluid comprises a sample microfluidic channel configured to receive the stream of particulate containing fluid, a guidance microfluidic channel having a polygonal cross-sectional area and configured to receive a stream of guidance fluid, and a common microfluidic channel having a polygonal cross sectional area formed by the merging of the sample microfluidic channel and the guidance 10 microfluidic channel at an oblique angle along only part of one or more sides of the guidance microfluidic channel, and a detection zone disposed in the common microfluidic channel having one or more sensors. The merging of the sample microfluidic channel and the guidance microfluidic channel is configured to provide a composite fluid stream containing a focussed beam of particulates that is disposed asymmetrically in the common microfluidic channel 15 adjacent a corner or side of the common microfluidic channel and wherein the one or more sensors are configured for sensing a characteristic of the focussed beam of particulates in the common channel.

公开(公告)号：US20190201889A1

公开(公告)日：2019-07-04

申请号：US16139317

申请日：2018-09-24

Applicant: CytoSaver LLC

Inventor： Matthew B. HALE

IPC: B01L3/00 ,  G01N1/40 ,  B01J8/00 ,  G01N1/34

CPC classification number: B01L3/50255 ,  B01D61/18 ,  B01D63/16 ,  B01D2315/02 ,  B01J8/006 ,  B01L2200/028 ,  B01L2200/0631 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/0409 ,  G01N1/34 ,  G01N1/40 ,  G01N1/4005 ,  G01N1/4077 ,  G01N2001/4088 ,  G01N2035/00495 ,  Y10T436/255

Abstract: A well plate includes a including a top portion, a bottom portion and a membrane disposed between the top portion and the bottom portion. The top portion defines a sample well in fluid communication with an opening defined by the membrane and in fluid communication with a reservoir defined by the bottom portion. The well plate is configured to be used in a centrifugation process of a test sample including a sample material and a wash liquid. The test sample configured to be received within the sample well and the reservoir. The membrane configured to filter the wash liquid from the test sample during the centrifugation process such that the wash liquid can pass from the reservoir, through the membrane and can be captured within a collection chamber while the sample material remains within the reservoir.

公开(公告)号：US20190134631A1

公开(公告)日：2019-05-09

申请号：US16237158

申请日：2018-12-31

Applicant: Alexandra Ros ,  Bahige G. Abdallah

Inventor： Alexandra Ros ,  Bahige G. Abdallah

IPC: B01L3/00 ,  G01N1/38

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0848 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/123 ,  B01L2300/16 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0655 ,  G01N1/38

Abstract: A microfluidic apparatus, systems and methods for microfluidic crystallization based on gradient mixing. In one embodiment, the apparatus includes (a) a first layer, (b) a plurality of first channels and a plurality of vacuum chambers both arranged in the first layer, where the plurality of vacuum chambers are each coupled to at least one of the first channels, (c) a membrane having first and second surfaces, where the first surface of the membrane is coupled to the first layer, (d) a second layer coupled to the second surface of the membrane, (e) a plurality of wells and a plurality of second channels both arranged in the second layer, where the wells are each coupled to at least one of the plurality of second channels and (f) a plurality of barrier walls each disposed in the plurality of second channels and arranged opposite to one of the plurality of vacuum chambers.

公开(公告)号：US20190078994A1

公开(公告)日：2019-03-14

申请号：US16085077

申请日：2017-03-16

Applicant: Georgia Tech Research Corporation

Inventor： Krishnendu Roy ,  Kirsten Parratt

IPC: G01N15/14 ,  G01N33/569 ,  B01L3/00 ,  G01N21/64

CPC classification number: G01N15/147 ,  B01L3/502761 ,  B01L2200/0652 ,  G01N15/1434 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/6486 ,  G01N33/50 ,  G01N33/569 ,  G01N2015/1006 ,  G01N2015/1445 ,  G01N2015/149

Abstract: Embodiments of the present disclosure can comprise a method for multiplexed analysis. The method can comprise acquiring interrogation data associated with a microstructure in a population and analyzing the microstructure based on the interrogation data. In some embodiments, the microstructure can have a different shape than at least another microstructure in the population and comprise a plurality of cells. Additionally, the acquiring the interrogation data can comprise acquiring interrogation data of microstructures in a population at a structure concentration of at least 100 microstructures/μL. Therefore, in some embodiments, acquiring the interrogation data can comprises flowing the population through a flow cytometer.

公开(公告)号：US20180364148A1

公开(公告)日：2018-12-20

申请号：US16048104

申请日：2018-07-27

Applicant: Celsee, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Christopher Siemer ,  Saedeh Sepehri Javdani

IPC: G01N15/14 ,  B01L3/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  C12M1/00 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/1484 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N2015/0065 ,  G01N2015/1006

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US20180355350A1

公开(公告)日：2018-12-13

申请号：US15996222

申请日：2018-06-01

Applicant: Raindance Technologies, Inc.

Inventor： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  G01N33/68 ,  B01F13/00 ,  G01N33/543 ,  G01N33/536 ,  C40B70/00 ,  C40B60/12 ,  C40B60/08 ,  C40B50/08 ,  C40B40/04 ,  C40B30/04 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/686 ,  C07K1/04 ,  B82Y30/00 ,  B82Y5/00 ,  B01L3/00 ,  B01J19/00 ,  G01N33/542 ,  G01N21/64 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6804 ,  B01L7/00 ,  B01F3/08

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US20180304222A1

公开(公告)日：2018-10-25

申请号：US15991600

申请日：2018-05-29

Applicant: President and Fellows of Harvard College ,  Vilnius University

Inventor： David A. Weitz ,  Allon Moshe Klein ,  Ilke Akartuna ,  Linas Mazutis ,  Marc W. Kirschner

IPC: B01J19/00 ,  B01F13/00

CPC classification number: B01J19/0046 ,  B01F13/0062 ,  B01J2219/00585 ,  B01J2219/00722 ,  B01L3/502761 ,  B01L3/502776 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2300/021 ,  B01L2300/0663 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/0883 ,  C12Q1/6806 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514

Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include “barcodes” or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.