公开(公告)号：US20190234968A1

公开(公告)日：2019-08-01

申请号：US16372819

申请日：2019-04-02

Applicant: Dignity Health

Inventor： Jonathan E. Valla

IPC: G01N33/68 ,  B01L3/00

CPC classification number: G01N33/6896 ,  B01L3/5021 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0822 ,  B01L2300/0838 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0409 ,  G01N2333/90216 ,  G01N2800/28 ,  G01N2800/2814 ,  G01N2800/2821

Abstract: The present invention relates to methods of diagnosing samples as well as various microfluidic, microcentrifuge and microfilter devices. In one embodiment, the present invention provides a method of diagnosing neurodegenerative diseases using mitochondrial and/or platelet samples. In another embodiment, the present invention provides a microfluidic device that selectively captures and analyzes a desired amount of target biological particle.

公开(公告)号：US20190218607A1

公开(公告)日：2019-07-18

申请号：US16213551

申请日：2018-12-07

Applicant: Massachusetts Institute of Technology

Inventor： J. Christopher Love ,  Todd Michael Gierahn ,  Alexander K. Shalek ,  Marc Havens Wadsworth ,  Travis K. Hughes

IPC: C12Q1/6874 ,  C12N15/10 ,  B01L3/00

CPC classification number: C12Q1/6874 ,  B01L3/5085 ,  B01L3/50855 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6806 ,  G03F7/2014 ,  C12Q2525/15 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2563/179 ,  C12Q2525/173

Abstract: This disclosure describes improvements to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, improved portability and ease of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays.

公开(公告)号：US20190193077A1

公开(公告)日：2019-06-27

申请号：US16288330

申请日：2019-02-28

Applicant: Click Diagnostics, Inc.

Inventor： Boris ANDREYEV ,  Keith E. MORAVICK ,  Brian CIOPYK ,  Victor BRIONES ,  Gregory LONEY ,  Adam DE LA ZERDA ,  Jesus CHING ,  Steven CHU ,  David SWENSON ,  Helen HUANG ,  Colin KELLY

IPC: B01L3/00 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01L3/5027 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1844 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0611 ,  B01L2400/0644

Abstract: A hand-held molecular diagnostic test device includes a housing, an amplification (or PCR) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (PCR) on the input sample. The detection module is configured to receive an output from the amplification module and a reagent formulated to produce a signal that indicates a presence of a target amplicon within the input sample. The amplification module and the detection module are integrated within the housing.

公开(公告)号：US20190001344A1

公开(公告)日：2019-01-03

申请号：US15965128

申请日：2018-04-27

Applicant: The General Hospital Corporation ,  GPB Scientific, LLC

Inventor： Lotien Richard Huang ,  Thomas A. Barber ,  Bruce L. Carvalho ,  Ravi Kapur ,  Paul Vernucci ,  Mehmet Toner ,  Zihua Wang

IPC: B03C1/30 ,  G01N1/40 ,  C12M1/00 ,  G01N33/50 ,  B01L3/00 ,  B03C1/32 ,  B33Y80/00 ,  G01N35/00

CPC classification number: B03C1/30 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0472 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C1/32 ,  B03C2201/18 ,  B33Y80/00 ,  C12M47/04 ,  C12M47/06 ,  G01N1/40 ,  G01N1/4077 ,  G01N33/5044 ,  G01N2035/00237 ,  Y10T137/0318 ,  Y10T137/8593 ,  Y10T436/25 ,  Y10T436/25375

Abstract: The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

公开(公告)号：US20180369804A1

公开(公告)日：2018-12-27

申请号：US16118547

申请日：2018-08-31

Applicant: FUJIFILM Corporation

Inventor： Yasuhisa KANEKO

IPC: B01L3/00

CPC classification number: B01L3/508 ,  B01L3/50851 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/12 ,  B01L2300/161

Abstract: Provided is a container for PCR enabling cell observation and a PCR treatment with a single container. In the container for PCR, a bottom surface 17a of an inside is flat, a shape of the bottom surface is a circular shape or a polygonal shape with four or more sides, a size of the bottom surface 17a is large so that a diameter L of an approximated circle circumscribing the bottom surface 17a is 0.05 mmϕ or more and 1 mmϕ or less, and an angle of an angle θ on a side surface side formed between a side surface 17b adjacent to the bottom surface 17a and the bottom surface 17a is 50° or more and 80° or less.

公开(公告)号：US20180355350A1

公开(公告)日：2018-12-13

申请号：US15996222

申请日：2018-06-01

Applicant: Raindance Technologies, Inc.

Inventor： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC: C12N15/10 ,  G01N33/68 ,  B01F13/00 ,  G01N33/543 ,  G01N33/536 ,  C40B70/00 ,  C40B60/12 ,  C40B60/08 ,  C40B50/08 ,  C40B40/04 ,  C40B30/04 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/686 ,  C07K1/04 ,  B82Y30/00 ,  B82Y5/00 ,  B01L3/00 ,  B01J19/00 ,  G01N33/542 ,  G01N21/64 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6804 ,  B01L7/00 ,  B01F3/08

CPC classification number: C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US20180321233A1

公开(公告)日：2018-11-08

申请号：US15968120

申请日：2018-05-01

Applicant: Sanguis LLC

Inventor： Daniel Zhang ,  Divyansh Agarwal ,  Prateek Agarwal

IPC: G01N33/543 ,  G01N33/58 ,  B01L3/00 ,  G01N15/14

CPC classification number: G01N33/54386 ,  B01L3/502715 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/027 ,  B01L2300/0816 ,  B01L2300/16 ,  B01L2400/0487 ,  B01L2400/06 ,  G01N15/1404 ,  G01N15/1484 ,  G01N33/582 ,  G01N2015/1486

Abstract: A system, a microfluidic chip and a method are provided for counting desired cells in a body fluid that allows for a reasonable range of error in exchange for fast and cheap diagnosis. The fluid containing the cells to be measured is immobilized on a microfluidic chip and stained and the cell count is determined from optical signals that measure the amount of stain acquired by the immobilized cells.

公开(公告)号：US20180318826A1

公开(公告)日：2018-11-08

申请号：US15969641

申请日：2018-05-02

Applicant: Massachusetts Institute of Technology

Inventor： Udayan Umapathi ,  Patrick Shin ,  Hiroshi Ishii

IPC: B01L3/02 ,  G01N35/10 ,  G01N35/00

CPC classification number: B01L3/0268 ,  B01L2200/0647 ,  B01L2400/0427 ,  G01N35/0099 ,  G01N35/1011

Abstract: An electrowetting-on-dielectric actuator may include an array of electrodes and a dielectric layer. The actuator may modulate voltage of the electrodes in such a way that time-varying electric fields created by the electrodes cause droplets of liquid to move relative to the actuator. The electric fields may cause the droplets to press up against a bottom surface of the actuator while the droplets are under and touching the bottom surface. The droplets may then be released from the bottom surface and thereby deposited on an object being fabricated. The release of droplets may be repeated, one layer of droplets at a time, to fabricate the object. Multiple actuators may simultaneously organize droplets in layers. A robot may move the actuators, one at a time, into position for releasing the droplets.

公开(公告)号：US20180305747A1

公开(公告)日：2018-10-25

申请号：US16022360

申请日：2018-06-28

Applicant: Raindance Technologies, Inc.

Inventor： Darren Roy Link

IPC: C12Q1/6848 ,  G01N1/38 ,  C12Q1/686 ,  B01L3/00 ,  C12Q1/6827 ,  B01J19/00

CPC classification number: C12Q1/6848 ,  B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2525/191 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel

公开(公告)号：US20180305651A1

公开(公告)日：2018-10-25

申请号：US15768736

申请日：2016-10-19

Applicant: EMULATE, Inc. ,  CEDARS-SINAI MEDICAL CENTER

Inventor： S. Jordan Kerns ,  Norman Wen ,  Carolina Lucchesi ,  Christopher David Hinojosa ,  Jacob Fraser ,  Geraldine Hamilton ,  Gad Vatine ,  Samuel Sances ,  Clive Svendsen ,  Daniel Levner ,  Dhruv Sareen

IPC: C12M3/06 ,  C12N5/0793 ,  C12N5/071 ,  B01L3/00

CPC classification number: C12N5/0619 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2300/0861 ,  C12M23/16 ,  C12M25/02 ,  C12M35/08 ,  C12N5/0618 ,  C12N5/0622 ,  C12N5/069 ,  C12N5/0692 ,  C12N2502/081 ,  C12N2502/086 ,  C12N2502/28 ,  C12N2506/45 ,  C12N2531/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/56 ,  C12N2535/10 ,  C12N2539/00 ,  C12Q1/02

Abstract: The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.