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公开(公告)号：US20150368700A1
公开(公告)日：2015-12-24
申请号：US14812388
申请日：2015-07-29
申请人： Enzo Biochem, Inc.
IPC分类号： C12Q1/68
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
摘要翻译： 本发明提供了用于分析物检测，定量和扩增的新型组合物和方法。 分析物的核酸阵列和文库可有效地并入到这样的组合物和方法中。 使用通用检测元件，信号实体等来检测并且如果需要或期望量化分析物。 靶分析物的扩增也由本发明的组合物和方法提供。
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公开(公告)号：US20150343410A1
公开(公告)日：2015-12-03
申请号：US14738591
申请日：2015-06-12
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译： 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
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公开(公告)号：US20150336071A1
公开(公告)日：2015-11-26
申请号：US14812951
申请日：2015-07-29
发明人： David A. Weitz , Jeremy Agresti
CPC分类号： C12Q1/6876 , B01J13/04 , B01J19/0046 , B01J2219/00599 , B01J2219/00709 , B01J2219/00711 , B01J2219/00716 , B01J2219/00722 , C12Q1/6874
摘要： The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.
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公开(公告)号：US20150314259A1
公开(公告)日：2015-11-05
申请号：US14726325
申请日：2015-05-29
申请人： ILLUMINA, INC.
发明人： Michal LEBL , Michel PERBOST , Chad F. DEROSIER , Mark J. NIBBE , Steve R. BURGETT , David L. HEINER
CPC分类号： B01J19/22 , B01J14/005 , B01J19/0046 , B01J2219/00306 , B01J2219/00324 , B01J2219/00351 , B01J2219/0036 , B01J2219/00378 , B01J2219/0038 , B01J2219/00495 , B01J2219/00518 , B01J2219/00533 , B01J2219/00585 , B01J2219/00596 , B01J2219/00644 , B01J2219/00686 , B01J2219/00693 , B01J2219/00722 , B01L3/50851 , B01L7/5255 , B01L2300/069 , B01L2300/0812 , B01L2300/0829 , B01L2300/123
摘要： Disclosed herein are compositions, methods and systems for the processing of chemical reactions, such as the synthesis of polymers.
摘要翻译： 本文公开了用于处理化学反应的组合物，方法和系统，例如聚合物的合成。
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公开(公告)号：US09169513B2
公开(公告)日：2015-10-27
申请号：US14512693
申请日：2014-10-13
申请人： ILLUMINA, INC.
发明人： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
CPC分类号： C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
摘要翻译： 一种方法，包括（a）提供包含位点阵列的扩增试剂和具有不同靶核酸的溶液; 和（b）使扩增试剂反应以产生每个具有来自溶液的靶核酸的扩增子的克隆群的扩增位点。 反应可以包括以平均传输速率同时将核酸输送到位点，以及以平均扩增速率扩增转运到位点的核酸，其中平均扩增速率超过平均传输速率。 该反应可以包括从转运到每个位点的核酸产生第一扩增子，并产生来自核酸或第一扩增子的后续扩增子，其中后续扩增子产生的平均速率超过在 其中生成第一个扩增子。
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公开(公告)号：US09163280B2
公开(公告)日：2015-10-20
申请号：US10902564
申请日：2004-07-29
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
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公开(公告)号：US20150276089A1
公开(公告)日：2015-10-01
申请号：US14188664
申请日：2014-02-24
发明人： Marc Alexander Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake , Markus Enzelberger , Mark L. Adams , Carl L. Hansen
IPC分类号： F16K99/00
CPC分类号： B05D3/04 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/06 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B65B31/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6832 , C12Q1/6874 , C30B29/54 , F04B43/043 , F15C1/06 , F15C3/00 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T137/0379 , Y10T137/0424 , Y10T137/206 , Y10T137/2082 , Y10T137/2164 , Y10T137/2169 , Y10T137/2174 , Y10T137/2202 , Y10T137/2224 , Y10T137/3084 , Y10T137/7837 , Y10T137/86027 , Y10T156/10 , Y10T428/24479 , Y10T428/24612 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
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88.发明申请公开(公告)号：US20150233934A1
公开(公告)日：2015-08-20
申请号：US14607496
申请日：2015-01-28
发明人： Richard Ebright
IPC分类号： G01N33/58
CPC分类号： C40B20/04 , B01J19/0046 , B01J2219/00459 , B01J2219/005 , B01J2219/00545 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , C40B30/04 , C40B40/06 , C40B40/10 , C40B50/04 , C40B50/16 , C40B70/00 , G01N21/29 , G01N21/64 , G01N21/6458 , G01N33/54313 , G01N33/582 , G01N33/6845 , G01N2021/1765
摘要： A method and apparatus for the physico-chemical encoding of a collection of beaded resin (“beads”) to determine the chemical identity of bead-anchored compounds by in-situ interrogation of individual beads. The present invention provides method and apparatus to implement color-coding strategies in applications and including the ultrahigh-throughput screening of bead-based combinatorial compounds libraries as well as multiplexed diagnostic and environmental testing and other biochemical assays.
摘要翻译： 用于物理化学编码串珠树脂（“珠粒”）的方法和装置，以通过单独珠粒的原位询问来确定珠状锚定化合物的化学特性。 本发明提供了在应用中实现颜色编码策略的方法和装置，并且包括基于珠的组合化合物文库的超高通量筛选以及多重诊断和环境测试以及其他生物化学测定。
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89.发明申请公开(公告)号：US20150231591A1
公开(公告)日：2015-08-20
申请号：US14625146
申请日：2015-02-18
发明人： Toshiro MURAYAMA
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J2219/00283 , B01J2219/0031 , B01J2219/00344 , B01J2219/00495 , B01J2219/00585 , B01J2219/00599 , B01J2219/00722 , C12Q1/686
摘要： A nucleic acid amplification reaction vessel includes a first inner wall, and a second inner wall that is arranged opposite to the first inner wall, in which a distance between the first inner wall and the second inner wall is a length in which a nucleic acid amplification reaction solution comes into contact with both the first inner wall and the second inner wall when the nucleic acid amplification reaction solution is poured.
摘要翻译： 核酸扩增反应容器包括第一内壁和与第一内壁相对布置的第二内壁，其中第一内壁和第二内壁之间的距离是核酸扩增的长度 当注入核酸扩增反应溶液时，反应溶液与第一内壁和第二内壁都接触。
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公开(公告)号：US20150191784A1
公开(公告)日：2015-07-09
申请号：US14593749
申请日：2015-01-09
发明人： David COX , Marc HABERSTROH , Patrick KINNEY , Albert CARRILLO , Jon HOSHIZAKI , Maryam SHARIATI , Howard KING , Joe Y. LEE , Matthew CHAN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , B01F13/0059 , B01F13/0222 , B01J19/0046 , B01J2219/00317 , B01J2219/00466 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/502707 , B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , C12Q1/6874 , G01N35/00069 , G01N35/1002 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
摘要： Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast-indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser. For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast-indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelengths are also provided herein.
摘要翻译： 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括具有多个激发和发射滤波器的快速分度滤光轮，该激发和发射滤光片能够快速旋转进入激发源（例如，弧光灯，激光器） 轮可以被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。该系统还可以被配置为考虑快速启动和停止快速索引所产生的振动 滤光轮以及由其他光源产生的振动。本文还提供了在多个波长下快速成像样品的各种方法。
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