公开(公告)号：US20210053058A1

公开(公告)日：2021-02-25

申请号：US16994289

申请日：2020-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon HOSHIZAKI ,  Patrick LEUNG ,  David MARRAN ,  Mark REED

IPC: B01L3/00

Abstract: An apparatus includes a fluidic coupler including an opening. A first port is in fluid communication with the opening and is to interface with an inlet of a flow cell of a sensor device. A second port is to interface with an outlet of the flow cell of the sensor device. A third port is in fluidic communication with the second port. The apparatus further includes a mechanical assembly moveable between a first position and a second position. The fluidic coupler is secured to the flow cell of the sensor device in the first position. The fluidic coupler is disengaged from the flow cell of the sensor device in the second position.

公开(公告)号：US20230226548A1

公开(公告)日：2023-07-20

申请号：US18107887

申请日：2023-02-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon HOSHIZAKI ,  Patrick LEUNG ,  David MARRAN ,  Mark REED

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2300/0816

Abstract: A fluidic coupler to engage a plurality of flow cells of a sensor device includes a body and a plurality of fluidics interfaces formed in the body. Each fluidic interface of the plurality of fluidics interfaces includes an opening, a first port in fluid communication with the opening, a second port, and a third port in fluidic communication with the second port.

公开(公告)号：US20150191784A1

公开(公告)日：2015-07-09

申请号：US14593749

申请日：2015-01-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David COX ,  Marc HABERSTROH ,  Patrick KINNEY ,  Albert CARRILLO ,  Jon HOSHIZAKI ,  Maryam SHARIATI ,  Howard KING ,  Joe Y. LEE ,  Matthew CHAN

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast-indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser. For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast-indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelengths are also provided herein.

Abstract translation: 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括具有多个激发和发射滤波器的快速分度滤光轮，该激发和发射滤光片能够快速旋转进入激发源（例如，弧光灯，激光器） 轮可以被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。该系统还可以被配置为考虑快速启动和停止快速索引所产生的振动 滤光轮以及由其他光源产生的振动。本文还提供了在多个波长下快速成像样品的各种方法。