-
公开(公告)号：US09498763B2
公开(公告)日：2016-11-22
申请号：US14738591
申请日：2015-06-12
Applicant: Illumina Cambridge Limited
Inventor： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC classification number: B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
-
公开(公告)号：US09303290B2
公开(公告)日：2016-04-05
申请号：US14295039
申请日：2014-06-03
Applicant: Illumina Cambridge Limited
Inventor： Milan Fedurco , Anthony Romieu , Gerardo Turcatti
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2563/107 , C12Q2527/125 , C12Q2523/107
Abstract: The invention relates to an additive which can be added to buffers used in nucleotide detection processes and improved methods of nucleic acid sequencing using this additive. In particular the invention relates to use of the additive to improve the efficiency of fluorescence-based multiple cycle nucleic acid sequencing reactions.
Abstract translation: 本发明涉及一种添加剂，其可加入用于核苷酸检测过程的缓冲液和使用该添加剂的改进的核酸测序方法。 特别地，本发明涉及添加剂用于提高基于荧光的多循环核酸测序反应的效率的用途。
-
公开(公告)号：US10710046B2
公开(公告)日：2020-07-14
申请号：US16010902
申请日：2018-06-18
Applicant: Illumina Cambridge Limited
Inventor： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC: C12Q1/6806 , C12Q1/6874 , B01J19/00 , C12Q1/6834
Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
-
公开(公告)号：US09970055B2
公开(公告)日：2018-05-15
申请号：US15050337
申请日：2016-02-22
Applicant: Illumina Cambridge Limited
Inventor： Milan Fedurco , Anthony Romieu , Gerardo Turcatti
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2563/107 , C12Q2527/125 , C12Q2523/107
Abstract: The invention relates to an additive which can be added to buffers used in nucleotide detection processes and improved methods of nucleic acid sequencing using this additive. In particular the invention relates to use of the additive to improve the efficiency of fluorescence-based multiple cycle nucleic acid sequencing reactions.
-
公开(公告)号：US20160208324A1
公开(公告)日：2016-07-21
申请号：US15050337
申请日：2016-02-22
Applicant: Illumina Cambridge Limited
Inventor： Milan Fedurco , Anthony Romieu , Gerardo Turcatti
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2563/107 , C12Q2527/125 , C12Q2523/107
Abstract: The invention relates to an additive which can be added to buffers used in nucleotide detection processes and improved methods of nucleic acid sequencing using this additive. In particular the invention relates to use of the additive to improve the efficiency of fluorescence-based multiple cycle nucleic acid sequencing reactions.
Abstract translation: 本发明涉及一种添加剂，其可加入用于核苷酸检测过程的缓冲液和使用该添加剂的改进的核酸测序方法。 特别地，本发明涉及添加剂用于提高基于荧光的多循环核酸测序反应的效率的用途。
-
Patent Agency Ranking
公开(公告)号：US20140378323A1
公开(公告)日：2014-12-25
申请号：US14295039
申请日：2014-06-03
Applicant: ILLUMINA CAMBRIDGE LIMITED
Inventor： Milan Fedurco , Anthony Romieu , Gerardo Turcatti
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2563/107 , C12Q2527/125 , C12Q2523/107
Abstract: The invention relates to an additive which can be added to buffers used in nucleotide detection processes and improved methods of nucleic acid sequencing using this additive. In particular the invention relates to use of the additive to improve the efficiency of fluorescence-based multiple cycle nucleic acid sequencing reactions.
Abstract translation: 本发明涉及一种添加剂，其可加入用于核苷酸检测过程的缓冲液和使用该添加剂的改进的核酸测序方法。 特别地，本发明涉及添加剂用于提高基于荧光的多循环核酸测序反应的效率的用途。
-
公开(公告)号：US20180353926A1
公开(公告)日：2018-12-13
申请号：US16010902
申请日：2018-06-18
Applicant: Illumina Cambridge Limited
Inventor： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC: B01J19/00 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874
CPC classification number: B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
-
公开(公告)号：US09999866B2
公开(公告)日：2018-06-19
申请号：US15287077
申请日：2016-10-06
Applicant: Illumina Cambridge Limited
Inventor： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC classification number: B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
-
公开(公告)号：US20170021325A1
公开(公告)日：2017-01-26
申请号：US15287077
申请日：2016-10-06
Applicant: Illumina Cambridge Limited
Inventor： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC classification number: B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
Abstract translation: 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
-
公开(公告)号：US20150343410A1
公开(公告)日：2015-12-03
申请号：US14738591
申请日：2015-06-12
Applicant: Illumina Cambridge Limited
Inventor： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC classification number: B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
Abstract translation: 本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物 切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上 用于核酸测序反应的单链模板。
-
-
-
-
-
-
-
-
-
Search Results
10
records
(took 0.017 seconds)
