公开(公告)号：US20240253041A1

公开(公告)日：2024-08-01

申请号：US18567169

申请日：2021-12-15

申请人： TSINGHUA UNIVERSITY

发明人： Zheng YOU ,  Zeheng JIAO ,  Jingjing ZHAO ,  Yong HAN

IPC分类号： B01L3/00

CPC分类号： B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/046

摘要： A sorting device based on an electric spark cavitation bubble includes: a liquid flow subsystem including a sheath flow channel, a sample flow channel, and a main flow channel, where the main flow channel is divided into a waste flow channel and a collection flow channel via a bifurcation port; a detecting subsystem configured to collect a pulse signal excited by the cell sample, and convert the pulse signal into an electrical signal; a data acquiring and processing subsystem configured to acquire and analyze the electrical signal, and issue a sorting instruction based on an analysis result; and a cavitation bubble generating subsystem configured to generate the cavitation bubble according to the sorting instruction, where the cavitation bubble pushes a liquid to generate a jet, and the bifurcation port is located within a range corresponding to the jet nozzle.

公开(公告)号：US20240101999A1

公开(公告)日：2024-03-28

申请号：US18235205

申请日：2023-08-17

申请人： Thermo Fisher Scientific GENEART GmbH ,  LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

发明人： Thomas POEHMERER ,  Phillip KUHN ,  Frank NOTKA ,  Andreas ZEIDLER ,  Korbinian HEIL ,  Axel TREFZER ,  Geir FONNUM ,  Federico KATZEN ,  Kristian ANDERSSON ,  Xiquan LIANG

IPC分类号： C12N15/10 ,  B01L3/00 ,  B03C5/02 ,  C25B3/29 ,  C25B9/70 ,  C25B11/04 ,  C25B15/02

CPC分类号： C12N15/101 ,  B01L3/502761 ,  B03C5/026 ,  C25B3/29 ,  C25B9/70 ,  C25B11/04 ,  C25B15/02 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2400/046 ,  B01L2400/0487 ,  B03C2201/26

摘要： The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US20180319655A1

公开(公告)日：2018-11-08

申请号：US15772377

申请日：2016-02-29

申请人： Hewlett-Packard Development Company, L.P.

发明人： Garrett E. Clark ,  Michael W. Cumbie ,  Chien-Hua Chen

IPC分类号： B81B7/00 ,  B81B1/00 ,  B81C1/00 ,  B01L3/00 ,  B41J2/14

CPC分类号： B81B7/0083 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502784 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/1827 ,  B01L2300/1894 ,  B01L2400/0442 ,  B01L2400/046 ,  B41J2/1433 ,  B81B1/006 ,  B81B2203/0338 ,  B81C1/00119 ,  F04B19/006 ,  F04B43/046

摘要： A fluid propelling apparatus, including a plastic compound, a MEMS at least partially surrounded by the compound, and a heat sink next to the MEMS, to transfer heat away from the MEMS, wherein the heat sink is at least partly surrounded by the compound.

公开(公告)号：US20180023121A1

公开(公告)日：2018-01-25

申请号：US15699718

申请日：2017-09-08

申请人： KECK GRADUATE INSTITUTE OF APPLIED LIFE SCIENCES

发明人： Angelika Niemz ,  Anna Hickerson ,  Kristina Roskos ,  Hsiang-Wei Lu

IPC分类号： C12Q1/68 ,  F04B17/00 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/686 ,  B01L3/502723 ,  B01L3/50273 ,  B01L7/00 ,  B01L2300/0663 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  F04B17/00

摘要： A disposable and inexpensive biological diagnostic cartridge for the amplification and detection of nucleic acids includes a configuration having a reaction pouch for amplification which is compressed by a flexible pump pouch for detection of the amplified reaction.

公开(公告)号：US09797006B2

公开(公告)日：2017-10-24

申请号：US13860453

申请日：2013-04-10

申请人： Keck Graduate Institute of Applied Life Sciences

发明人： Angelika Niemz ,  Anna Hickerson ,  Kristina Roskos ,  Hsiang-Wei Lu

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  F04B17/00

CPC分类号： C12Q1/686 ,  B01L3/502723 ,  B01L3/50273 ,  B01L7/00 ,  B01L2300/0663 ,  B01L2400/0421 ,  B01L2400/046 ,  B01L2400/0481 ,  F04B17/00

摘要： A disposable and inexpensive biological diagnostic cartridge for the amplification and detection of nucleic acids includes a configuration having a reaction pouch for amplification which is compressed by a flexible pump pouch for detection of the amplified reaction.

公开(公告)号：US20160011114A1

公开(公告)日：2016-01-14

申请号：US14769885

申请日：2014-02-20

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATIO ,  USHIO DENKI KABUSHIKI KAISHA

发明人： Yuji OKI ,  Kinichi MORITA

IPC分类号： G01N21/64 ,  G01N33/53 ,  G01N33/536 ,  B01L3/00 ,  G01N33/569

CPC分类号： G01N21/6428 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/0647 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2400/0403 ,  B01L2400/0442 ,  B01L2400/046 ,  G01N21/03 ,  G01N21/05 ,  G01N33/5304 ,  G01N33/536 ,  G01N33/56966 ,  G01N2021/6482 ,  G01N2201/0245

摘要： In order to carry out various analyses at high accuracy in a site where analysis is needed using a disposable microchip, the microchip is disposed on a display of a tablet terminal device, and a sample or specimen is dropped into a flow path of the microchip to trigger a reaction of the sample in the microchip. Light emitted from the display of the tablet terminal device is applied to the microchip, and the reaction caused by the applied light in the microchip is measured. When an induced fluorescence method using the applied light is adopted as a measuring method, fluorescence that corresponds to the reaction is observed, and detected by, for example, a built-in camera of the tablet terminal device. The detection signal is analyzed by an arithmetic unit of the tablet terminal device.

摘要翻译： 为了在使用一次性微芯片的需要分析的场所以高精度进行各种分析，将微芯片设置在平板电脑终端装置的显示器上，将样品或样品落入微芯片的流路中， 触发微芯片中样品的反应。 从平板电脑终端装置的显示器发射的光被施加到微芯片，并且测量由微芯片中的施加的光引起的反应。 当采用使用所施加的光的诱导荧光方法作为测量方法时，观察到与反应相对应的荧光，并且通过例如平板电脑终端装置的内置照相机进行检测。 检测信号由平板电脑终端装置的算术单元分析。

公开(公告)号：US08695640B2

公开(公告)日：2014-04-15

申请号：US13360261

申请日：2012-01-27

申请人： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake

发明人： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake

IPC分类号： F15C1/06

CPC分类号： B29C51/00 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  B81B5/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  B81C2201/034 ,  C12Q1/6874 ,  F04B43/043 ,  F15C1/06 ,  F15C5/00 ,  F16K11/20 ,  F16K13/00 ,  F16K31/126 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  Y10T137/0491 ,  Y10T137/0497 ,  Y10T137/2224 ,  Y10T137/87249 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24744 ,  C12Q2535/125

摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

摘要翻译： 一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

公开(公告)号：US08656958B2

公开(公告)日：2014-02-25

申请号：US11932263

申请日：2007-10-31

申请人： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake ,  Markus M. Enzelberger ,  Mark L. Adams ,  Carl L. Hansen

发明人： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake ,  Markus M. Enzelberger ,  Mark L. Adams ,  Carl L. Hansen

IPC分类号： F15C3/00

CPC分类号： B05D3/04 ,  B01J2219/00355 ,  B01J2219/00378 ,  B01J2219/00396 ,  B01J2219/00398 ,  B01J2219/00439 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/54 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/18 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/06 ,  B01L2400/0655 ,  B01L2400/0688 ,  B32B2037/1081 ,  B65B31/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  C12Q1/6832 ,  C12Q1/6874 ,  C30B29/54 ,  F04B43/043 ,  F15C1/06 ,  F15C3/00 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T137/0324 ,  Y10T137/0379 ,  Y10T137/0424 ,  Y10T137/206 ,  Y10T137/2082 ,  Y10T137/2164 ,  Y10T137/2169 ,  Y10T137/2174 ,  Y10T137/2202 ,  Y10T137/2224 ,  Y10T137/3084 ,  Y10T137/7837 ,  Y10T137/86027 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24612 ,  C12Q2535/125

摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

公开(公告)号：US08480053B2

公开(公告)日：2013-07-09

申请号：US11514396

申请日：2006-08-30

申请人： James R. Heath ,  Michael Van Dam ,  Arkadij Elizarov

发明人： James R. Heath ,  Michael Van Dam ,  Arkadij Elizarov

IPC分类号： F16K7/04

CPC分类号： B01L3/502738 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502723 ,  B01L3/567 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/046 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0026 ,  F16K99/0034 ,  F16K99/0046 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/8593

摘要： Mechanical actuation of valves in flexible fluidic structures allows for the regulation of fluid flow. In accordance with the disclosure herein, a fluidic structure is provided wherein mechanical actuation is conferred using a pin to actuate a flexible layer to occlude fluid flow in a fluid channel.

摘要翻译： 柔性流体结构中的阀的机械致动允许调节流体流动。 根据本文的公开内容，提供流体结构，其中使用销赋予机械致动来致动柔性层以阻塞流体通道中的流体流动。

公开(公告)号：US08104515B2

公开(公告)日：2012-01-31

申请号：US12540994

申请日：2009-08-13

申请人： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake

发明人： Marc A. Unger ,  Hou-Pu Chou ,  Todd A. Thorsen ,  Axel Scherer ,  Stephen R. Quake

IPC分类号： F15C1/06

CPC分类号： B29C51/00 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/046 ,  B01L2400/0481 ,  B01L2400/0655 ,  B33Y80/00 ,  B81B5/00 ,  B81B2201/036 ,  B81B2201/054 ,  B81C1/00119 ,  B81C2201/019 ,  B81C2201/034 ,  C12Q1/6874 ,  F04B43/043 ,  F15C1/06 ,  F15C5/00 ,  F16K11/20 ,  F16K13/00 ,  F16K31/126 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0046 ,  F16K99/0048 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0094 ,  Y10T137/0491 ,  Y10T137/0497 ,  Y10T137/2224 ,  Y10T137/87249 ,  Y10T156/10 ,  Y10T428/24479 ,  Y10T428/24744 ,  C12Q2535/125

摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.