公开(公告)号：US12129474B2

公开(公告)日：2024-10-29

申请号：US18182701

申请日：2023-03-13

申请人： Altria Client Services LLC

发明人： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James Strickland

IPC分类号： C12N15/82 ,  A01H4/00 ,  A24B13/00 ,  A24B15/10 ,  A24B15/24 ,  B01F33/45 ,  B01F33/452 ,  B01F33/501 ,  B01F35/75 ,  B01L3/00 ,  C07K14/415 ,  C12N9/04 ,  G01N1/38 ,  H02N11/00 ,  G01N35/00 ,  G01N35/10

CPC分类号： C12N15/8243 ,  A01H4/005 ,  A01H4/008 ,  A24B13/00 ,  A24B15/10 ,  A24B15/245 ,  B01F33/45 ,  B01F33/452 ,  B01F33/50112 ,  B01F35/754251 ,  B01L3/502 ,  B01L3/50825 ,  C07K14/415 ,  C12N9/0006 ,  C12N15/8205 ,  C12N15/8225 ,  C12N15/825 ,  G01N1/38 ,  H02N11/006 ,  B01L2200/0684 ,  B01L2300/043 ,  B01L2300/0672 ,  B01L2300/0867 ,  B01L2400/0403 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0683 ,  G01N2035/00534 ,  G01N35/1079

摘要： The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

公开(公告)号：US20240310390A1

公开(公告)日：2024-09-19

申请号：US18668683

申请日：2024-05-20

申请人： ORANGE BIOMED LTD., CO.

发明人： Ung Hyeon Ko ,  Seung Jin Kang ,  Eun Young Park

IPC分类号： G01N33/72 ,  B01L3/00 ,  G01N33/49 ,  G01N33/80

CPC分类号： G01N33/726 ,  B01L3/502715 ,  G01N33/49 ,  G01N33/723 ,  G01N33/80 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0403

摘要： The present disclosure relates to systems and methods for measuring glycated A1c hemoglobin. A glycated hemoglobin level measuring system includes a sample testing apparatus having a microchannel that compresses a blood sample traveling through, a first pair of electrodes coupled to the microchannel, and a second pair of electrodes coupled to the microchannel. The glycated hemoglobin level measuring system further includes an analysis apparatus having sensors coupled to the first and second pairs of electrodes and configured to calculate a travel time taken by a red blood cell to pass through the first and second pairs of electrodes. The glycated hemoglobin level measuring system can use the travel time to measure a rigidity of the red blood cells and the corresponding glycated hemoglobin level.

公开(公告)号：US12064769B2

公开(公告)日：2024-08-20

申请号：US18087438

申请日：2022-12-22

申请人： SomaLogic Operating Co., Inc.

发明人： Jason Paul Cleveland ,  Jon Monserud ,  Barry Patrick John Vant-Hull ,  Alex Kislukhin

IPC分类号： B01L3/00 ,  B01L7/00 ,  B01L9/00

CPC分类号： B01L3/50851 ,  B01L7/52 ,  B01L9/523 ,  B01L2300/0663 ,  B01L2300/0829 ,  B01L2300/18 ,  B01L2400/0403

摘要： Systems and methods for conducting surface-mediated chemical and/or biochemical reactions within an enclosed chamber are disclosed. Systems and methods of the present disclosure may be used in conducting hybridization reactions of biopolymers. In some examples, an improved method for mixing thin films of solutions in a hybridization chamber includes altering the direction of mixing at least once over the course of a reaction. In some examples, an improved method for mixing thin films of solutions in a hybridization chamber includes altering the speed of mixing at least once over the course of a reaction. In some examples, an improved method for mixing thin films of solutions in a hybridization chamber includes altering the speed of mixing and the direction of mixing at least once over the course of a reaction.

公开(公告)号：US11905550B2

公开(公告)日：2024-02-20

申请号：US16585729

申请日：2019-09-27

申请人： Commissariat a l'energie atomique et aux energies alternatives ,  SERVICE DEPARTEMENTAL METROPOLITAIN D'INCENDIE ET DE SECOURS (SDMIS)

发明人： Anne-Gaelle Bourdat ,  Gregory Wenisch

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  B01L3/00 ,  C12N15/10 ,  G01N1/28

CPC分类号： C12Q1/6806 ,  B01L3/502746 ,  B01L3/502753 ,  C12N15/1017 ,  G01N1/286 ,  B01L2300/0681 ,  B01L2400/0403

摘要： A process for preparing a biological sample including biological species, implemented in a preparation system, the preparation system including a device that includes: a housing, a first channel provided in the housing, a second channel provided in the housing, a chamber into which the first channel and the second channel open, a filter separating the chamber into two distinct spaces, the process including the following steps: injection of the biological sample in the form of a fluid via the first channel to concentrate biological species in the first space of the chamber of the device, injection of an immunological buffer fluid via the second channel of the device so as to at least partially elute the biological species with the immunological buffer fluid.

公开(公告)号：US11789017B2

公开(公告)日：2023-10-17

申请号：US17119732

申请日：2020-12-11

申请人： Magnolia Medical Technologies, Inc.

发明人： Gregory J. Bullington ,  Jay M. Miazga ,  Shan E. Gaw ,  Paul Goldenbaum ,  Dylan Guelig

IPC分类号： C12N15/10 ,  B01L3/00 ,  G01N21/3504 ,  G01N33/00 ,  G01N33/574 ,  G01N21/64 ,  A61K45/06 ,  G01N21/35 ,  G01N33/50 ,  G01N33/543 ,  B01L3/02 ,  G01N33/53

CPC分类号： G01N33/54388 ,  B01L3/0217 ,  B01L3/502715 ,  G01N33/5304 ,  B01L2300/0819 ,  B01L2400/0403

摘要： A system includes a fluid transfer device and a lateral flow assay device. The fluid transfer device has an inlet fluidically coupleable to a bodily fluid source, an outlet fluidically coupleable to a sample reservoir, and a sequestration chamber configured to receive an initial volume of bodily fluid. The fluid transfer device can be transitioned between (1) a first state with the sequestration chamber in fluid communication with the inlet to receive the initial volume, (2) a second state with the outlet in fluid communication with the inlet to receive a subsequent flow of bodily fluid, and (3) a third state with the lateral flow assay device in fluid communication with the sequestration chamber to receive a portion of the initial volume of bodily fluid. The lateral flow assay device configured to provide an indication associated with a presence of a target analyte in the bodily fluid.

公开(公告)号：US11772093B2

公开(公告)日：2023-10-03

申请号：US18062011

申请日：2022-12-05

申请人： mirOculus Inc.

发明人： Mais Jehan Jebrail ,  Foteini Christodoulou ,  Ana Eugenia Carvajal ,  Eduardo Cervantes ,  Rohit Lal ,  Mark Lewis

IPC分类号： B01L3/00 ,  C12Q1/6874

CPC分类号： B01L3/502784 ,  C12Q1/6874 ,  B01L2200/0642 ,  B01L2300/0816 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/022 ,  B01L2400/0403

摘要： Methods and apparatuses for mechanically controlling microfluidic movement using a force applicator and an elastically deformable sheet are described herein. These apparatuses may include a mechanical microfluidics actuator devices and a cartridge. A microfluidic droplet may be moved or displaced within an air gap of the cartridge by applying a compressive force locally and selectively reduce the gap width of the air gap near the microfluidic droplet causing the microfluidic droplet to move toward the reduced gap. Compressive forces may also be used to divide, join, mix or perform other operations on the microfluidic droplets.

公开(公告)号：US20160158752A1

公开(公告)日：2016-06-09

申请号：US14930054

申请日：2015-11-02

申请人： The Regents of the University of California

发明人： Pei-Yu E. Chiou ,  Ting-Hsiang S. Wu ,  Sung-Yong Park ,  Michael A. Teitell

IPC分类号： B01L3/00

CPC分类号： B01L3/502715 ,  B01J13/04 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/0403 ,  B01L2400/0442 ,  G01N15/10 ,  G01N21/64 ,  G01N29/2418 ,  G01N2015/1481 ,  G01N2291/02433 ,  G05D11/13 ,  Y10T137/0318 ,  Y10T137/2496

摘要： Methods and devices for the formation of droplets of a first fluid in a second fluid and the encapsulation of particles or cells within such droplets are disclosed. Impetus for droplet formation is provided by the creation of a transient bubble, which may be induced using a pulsed laser. Droplet volume and the frequency at which droplets are formed can be controlled by modulation of the pulsed laser. The disclosed methods and devices are particularly suitable for use in microfluidic devices.

摘要翻译： 公开了用于在第二流体中形成第一流体的液滴的方法和装置以及在这些液滴内的颗粒或细胞的包封。 通过产生可以使用脉冲激光诱发的瞬态气泡来提供液滴形成的动力。 可以通过调制脉冲激光来控制液滴体积和形成液滴的频率。 所公开的方法和装置特别适用于微流体装置。

公开(公告)号：US07208320B2

公开(公告)日：2007-04-24

申请号：US10318979

申请日：2002-12-13

申请人： Andreas Manz ,  Luc J. Bousse

发明人： Andreas Manz ,  Luc J. Bousse

IPC分类号： G01N1/10 ,  B01L3/02 ,  B01D57/02

CPC分类号： B01L3/5027 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0403 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/0622 ,  G01N35/08 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/2575

摘要： Microfluidic devices and systems for affecting the serial to parallel conversion of materials introduced into the device or system. Material or materials to be converted from a serial orientation, e.g., a single channel, into a parallel orientation, e.g., multiple channels, are introduced into an open chamber or field in which containing flows of materials maintain the cohesiveness of the sample material plugs serially introduced into the open chamber. The sample material or materials are then redirected in the chamber toward and into a plurality of parallel channels that also communicate with the chamber.

摘要翻译： 用于影响引入设备或系统的材料的串行到并行转换的微流控器件和系统。 要从串行方向（例如单个通道）转换为平行取向（例如多个通道）的材料或材料被引入到开放的室或场中，其中包含材料流保持样品材料塞的连续性串联 引入开放室。 样品材料然后在室中被重新定向并且进入也与室连通的多个平行通道。

公开(公告)号：US06806052B2

公开(公告)日：2004-10-19

申请号：US10124884

申请日：2002-04-18

申请人： John Bridgham ,  Kevin P. Corcoran ,  George S. Golda ,  Michael C. Pallas ,  Sydney Brenner

发明人： John Bridgham ,  Kevin P. Corcoran ,  George S. Golda ,  Michael C. Pallas ,  Sydney Brenner

IPC分类号： C12Q168

CPC分类号： C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/00317 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/0622 ,  B01L2400/0644 ,  C40B60/14 ,  G01N15/1456 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/253 ,  G01N2015/0015 ,  G01N2015/149 ,  C12Q2565/518 ,  C12Q2525/161 ,  C12Q2521/313 ,  C12Q2525/191

摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

摘要翻译： 提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。 每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒进行， 微处理器控制。 响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。 通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。 本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。

公开(公告)号：US20030082081A1

公开(公告)日：2003-05-01

申请号：US10277754

申请日：2002-10-23

申请人： COMMISSARIAT A L'ENERGIE ATOMIQUE

发明人： Yves Fouillet ,  Nicolas Sarrut ,  Antoine Gruss

IPC分类号： B01L003/00 ,  G05D001/00

CPC分类号： B01F13/0071 ,  B01F13/0076 ,  B01F13/0077 ,  B01F15/00123 ,  B01F15/00344 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00306 ,  B01J2219/00353 ,  B01J2219/00389 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/0068 ,  B01J2219/00783 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01J2219/0093 ,  B01L3/563 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/1822 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/06 ,  B01L2400/082 ,  C40B60/14 ,  G01N35/08 ,  G01N2035/00237 ,  Y10T137/0391

摘要： The invention relates to a microfluidic device for injecting series of mobile reaction chambers (102, 103) having non-miscible segmenters (101) in microchannels (21 to 26), comprising: injection means (10) for injecting into microreaction channels alternatingly and in parallel liquid to form mobile reaction chambers and liquid for forming the segmenters; means for controlling the progression of one of the two liquids, applied to act on one zone (31) of each microchannel delimiting an injection volume of said liquid; the control means being able to cause stopping or slowing of the progression of said liquid over the zone of each microchannel by exerting an action based on a physico-chemical property of the liquid and said action not affecting the other liquid.

摘要翻译： 本发明涉及一种用于在微通道（21至26）中注入具有不可混合分段器（101）的系列移动反应室（102,103）的微流体装置，包括：注入装置（10），用于交替地和在 平行液体形成流动反应室和用于形成分段器的液体; 用于控制两个液体中的一个液体的进行的装置，用于作用于限定所述液体的喷射体积的每个微通道的一个区域（31）; 所述控制装置能够通过基于液体的物理化学性质和不影响其它液体的作用施加作用而使所述液体在每个微通道的区域上的进展停止或减慢。