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    Method for preparing antibody monolayers which have controlled orientation using peptide hybrid
    31.
    发明授权
    Method for preparing antibody monolayers which have controlled orientation using peptide hybrid 有权
    使用肽杂交制备具有受控取向的抗体单层的方法

    公开(公告)号：US08236509B2

    公开(公告)日：2012-08-07

    申请号：US12679982

    申请日：2007-10-18

    Applicant: Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

    Inventor： Sang Jeon Chung ,  Bong Hyun Chung ,  Yongwon Jung ,  Hyo Jin Kang ,  Jeong Min Lee

    IPC: G01N33/53

    CPC classification number: C07K17/00 ,  C07K7/02 ,  C07K7/08 ,  G01N33/54353 ,  G01N33/54393

    Abstract: The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinity. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.

    Abstract translation: 本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说，用于蛋白质固定的肽杂交体，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性 蛋白质类型被设计为在固体底物和固定的蛋白质之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。 用于本发明的蛋白质固定化的肽杂合物有助于控制抗体在各种固体表面上的取向并且固定不同来源的各种抗体或具有不同亲和力的不同同种型。 因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

    METHOD FOR DETECTION AND QUANTIFICATION OF PLK1 EXPRESSION AND ACTIVITY
    32.
    发明申请
    METHOD FOR DETECTION AND QUANTIFICATION OF PLK1 EXPRESSION AND ACTIVITY 审中-公开
    PLK1表达和活性的检测和定量方法

    公开(公告)号：US20110076693A1

    公开(公告)日：2011-03-31

    申请号：US12992887

    申请日：2009-05-15

    Applicant: Kyung S. Lee ,  Jung Eun Park

    Inventor： Kyung S. Lee ,  Jung Eun Park

    IPC: G01N33/573 ,  C07K14/00 ,  C07K7/06 ,  C07K7/08 ,  C12Q1/48 ,  G01N33/53

    CPC classification number: C07K14/00 ,  C07K17/00 ,  C12N9/1229 ,  C12Q1/485 ,  G01N33/57484 ,  G01N2500/04 ,  G01N2800/56

    Abstract: Isolated peptide substrates of Plk1 and nucleic acids encoding these peptides are disclosed. The peptides include two to ten repeats of the amino acid sequence set forth as X1X2AX3X4X5PLHSTX6X7X8X9X10X11X12 (SEQ ID NO: 1), in which within each repeat X1, X2, X6, X7, X8, X9, X10, X11, and X12 are each independently any amino acid or no amino acid, and X3 and X4 are each independently any amino acid. Methods of using these peptides to detect Plk1 activity in a sample are also disclosed. In some examples, the method includes contacting a sample with a disclosed peptide substrate of Plk1 in the presence of adenosine triphosphate, or an analog thereof, for a period of time sufficient for Plk1 to phosphorylate the PBIPtide. The presence and/or amount of the phosphorylated and/or the unphosphorylated peptide is detected, thereby detecting and/or quantitating Plk1 kinase activity in the sample.

    Abstract translation: 公开了Plk1的分离的肽底物和编码这些肽的核酸。 肽包括X1X2AX3X4X5PLHSTX6X7X8X9X10X11X12（SEQ ID NO：1）所示的氨基酸序列的2至10个重复，其中每个重复X1，X2，X6，X7，X8，X9，X10，X11和X12各自独立地 任何氨基酸或不含氨基酸，X3和X4各自独立地为任何氨基酸。 还公开了使用这些肽检测样品中Plk1活性的方法。 在一些实例中，该方法包括在三磷酸腺苷或其类似物的存在下将样品与公开的Plk1肽底物接触足以使Plk1磷酸化PBIPtide的时间。 检测磷酸化和/或未磷酸化肽的存在和/或量，从而检测和/或定量样品中的Plk1激酶活性。

    Mucin immobilized chromatography
    36.
    发明授权
    Mucin immobilized chromatography 有权
    粘蛋白固定色谱

    公开(公告)号：US07659240B2

    公开(公告)日：2010-02-09

    申请号：US10345453

    申请日：2003-01-14

    Applicant: Irina Kadiyala ,  Riccardo Panicucci

    Inventor： Irina Kadiyala ,  Riccardo Panicucci

    IPC: C07K14/00

    CPC classification number: G01N33/538 ,  C07K14/4727 ,  C07K17/00 ,  G01N2333/4725

    Abstract: An in vitro high-throughput screening method that models the absorption of drugs across the epithelial mucosa is provided. The invention discloses an in vitro mucin immobilized chromatography model to estimate drug permeability coefficients. The invention describes compositions for mucin immobilized chromatography media and methods to prepare this media. The invention also discloses a method to estimate in vitro drug permeability coefficients using mucin immobilized chromatography media. The invention also discloses methods to determine absorption processes in the digestive system and discloses methods of use for mucin chromatography media in column, batch, assay, diagnostic, or high-throughput screening analyses.

    Abstract translation: 提供了一种模拟通过上皮粘膜吸收药物的体外高通量筛选方法。 本发明公开了一种用于估计药物渗透系数的体外粘蛋白固定化色谱模型。 本发明描述了用于粘蛋白固定的色谱介质的组合物和制备该介质的方法。 本发明还公开了使用粘蛋白固定化色谱介质估计体外药物渗透系数的方法。 本发明还公开了确定消化系统中吸收过程的方法，并公开了在柱，批，测定，诊断或高通量筛选分析中使用粘蛋白色谱介质的方法。

    IMMUNOGENIC COMPOSITIONS AND METHODS OF USE
    37.
    发明申请
    IMMUNOGENIC COMPOSITIONS AND METHODS OF USE 有权

    公开(公告)号：US20100028410A1

    公开(公告)日：2010-02-04

    申请号：US12573366

    申请日：2009-10-05

    Applicant: Donald T. Haynie

    Inventor： Donald T. Haynie

    IPC: A61K9/70 ,  A61K9/50 ,  A61K39/00

    CPC classification number: C07K17/00 ,  B82Y30/00

    Abstract: Disclosed herein are immunogenic compositions comprising a multilayer film comprising two or more layers of polyelectrolytes, wherein adjacent layers comprise oppositely charged polyelectrolytes. A first layer polyelectrolyte comprises an antigenic polypeptide comprising one or more surface adsorption regions covalently linked to one or more antigenic determinant regions, wherein the antigenic polypeptide and the one or more surface adsorption regions have the same polarity. The immunogenic compositions may be employed in methods of eliciting an immune response in a vertebrate organism.

    Polypeptide scaffolds and use thereof
    38.
    发明授权
    Polypeptide scaffolds and use thereof 失效
    多肽支架及其应用

    公开(公告)号：US07569661B2

    公开(公告)日：2009-08-04

    申请号：US10512295

    申请日：2003-03-26

    Applicant: Lars Baltzer ,  Gunnar Dolphin ,  Bo Liedberg ,  Ingemar Lundström

    Inventor： Lars Baltzer ,  Gunnar Dolphin ,  Bo Liedberg ,  Ingemar Lundström

    IPC: A61K38/00

    CPC classification number: B82Y30/00 ,  C07K1/047 ,  C07K1/1077 ,  C07K14/001 ,  C07K17/00

    Abstract: Novel peptide having a sequence according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 are disclosed and also polypeptide scaffold consisting of a four helix bundle formed of two dimerized helix-loop-helix motifs, said helix-loop-helix motifs having sequences according to SEQ. ID. No. 1, SEQ. ID. No. 2 and/or SEQ. ID. No. 3 which may comprise a fluorescent probe at the side chain of Lys15 and a ligand with affinity for a target molecule at the side chain of Lys8 or Lys34.Also disclosed are polypeptide scaffolds for use in biosensing applications with or without the polypeptide scaffold anchored to a solid surface.

    Abstract translation: 具有SEQ ID NO： ID。 第1号，SEQ。 ID。 2和/或SEQ。 ID。 公开了3号，并且还包括由两个二聚的螺旋 - 环 - 螺旋基序形成的四个螺旋束组成的多肽支架，所述螺旋 - 环 - 螺旋基序具有根据SEQ ID NO： ID。 第1号，SEQ。 ID。 2和/或SEQ。 ID。 可以包含Lys15侧链上的荧光探针和对Lys8或Lys34侧链上的靶分子具有亲和力的配体。 还公开了用于生物感测应用的多肽支架，其具有或不具有固定到固体表面的多肽支架。

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