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公开(公告)号：US09327212B2
公开(公告)日：2016-05-03
申请号：US13497896
申请日：2010-09-23
申请人： Torbjorn Blank , Enrique Carredano , Karl Ekstrom , Jan-Erik Lynga , Roger Nordberg , Mike Peters , Gustav Rodrigo , Henrik Sandegren , Lisa Stack
发明人： Torbjorn Blank , Enrique Carredano , Karl Ekstrom , Jan-Erik Lynga , Roger Nordberg , Mike Peters , Gustav Rodrigo , Henrik Sandegren , Lisa Stack
CPC分类号： B01D15/166 , B01F15/0022 , B01F15/00227 , G01N30/34 , G01N2030/347 , G05D11/132
摘要： A method for preparing a liquid flow having pre-defined characteristics by mixing liquid flows of at least two different component stock solutions with each other comprises the steps of determining a selected property value for one or more of the stock solutions by sensing, in a flow of each stock solution separately, at least one characteristic related to the property value for the stock solution, and based on the determined property value or values, mixing the stock solution flows in mixing ratios giving the desired mixed liquid flow. A system for carrying out the method comprises sensor means and a control unit, wherein the control unit is arranged to evaluate the characteristics sensed by the sensor means and provide relative ratios of stock solutions required to obtain a mixed liquid flow having the pre-defined characteristics.
摘要翻译： 通过混合至少两种不同组分原料溶液的液体流相互混合制备具有预定特性的液体流的方法包括以下步骤：通过在流体中感测来确定一种或多种储备溶液的选定的特性值 分别存储与储备溶液的特性值相关的至少一个特征，并且基于所确定的特性值或值，以提供所需混合液体流的混合比混合储备液流。 用于执行该方法的系统包括传感器装置和控制单元，其中控制单元布置成评估由传感器装置感测到的特性，并提供获得具有预定特性的混合液流所需的储备溶液的相对比例 。
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公开(公告)号：US20130245224A1
公开(公告)日：2013-09-19
申请号：US13990135
申请日：2011-11-28
申请人： Tomas Bjorkman , Gustav Rodrigo
发明人： Tomas Bjorkman , Gustav Rodrigo
IPC分类号： C07K1/22
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/286 , B01J20/3208 , B01J20/3242 , B01J20/3274 , C07K16/065
摘要： The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the C-terminal most proline residue after the third alpha-helix.
摘要翻译： 本发明涉及从液体中分离一种或多种含免疫球蛋白的蛋白质的方法。 该方法包括首先使液体与包含固定在载体上的配体的分离基质接触; 允许含有免疫球蛋白的蛋白质通过与配体的相互作用吸附到基质上; 随后是洗涤吸附的含有蛋白质的免疫球蛋白的任选步骤; 并通过使基质与释放蛋白质的洗脱液接触来回收所述含有免疫球蛋白的蛋白质。 该方法改进了以前的分离方法，因为每种配体包含蛋白A结构域（E，D，A，B，C）或蛋白质Z或其功能变体中的一种或多种，其中至少一种 在第三个α-螺旋之后具有C末端最多脯氨酸残基取代的单体。
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3.发明授权Chromatography ligand comprising domain C from Staphylococcus aureus protein A for antibody isolation 有权包含来自金黄色葡萄球菌蛋白A的结构域C的色谱配体用于抗体分离公开(公告)号：US08329860B2
公开(公告)日：2012-12-11
申请号：US12443011
申请日：2007-09-27
申请人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
发明人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC分类号： C07K17/00
CPC分类号： B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
摘要： The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
摘要翻译： 本发明涉及一种色谱配体，其包含来自葡萄球菌蛋白A（SpA）的结构域C，或其功能片段或变体。 色谱配体具有在现场（CIP）条件下耐受苛刻清洁的有利能力，并且能够结合抗体的Fab片段。 配体可以具有末端偶联基团，例如精氨酸或半胱氨酸，以促进其与不溶性载体例如珠粒或膜的偶联。 本发明还涉及在分离抗体中使用配体的方法以及可以包括洗涤步骤和/或用碱再生的纯化方案。
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4.发明申请CHROMATOGRAPHY LIGAND COMPRISING DOMAIN C FROM STAPHYLOCOCCUS AUREUS PROTEIN A FOR ANTIBODY ISOLATION 有权含有葡萄球菌蛋白A的抗体分离体的C区的色谱配体公开(公告)号：US20100048876A1
公开(公告)日：2010-02-25
申请号：US12443011
申请日：2007-09-27
申请人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
发明人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
CPC分类号： B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
摘要： The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
摘要翻译： 本发明涉及一种色谱配体，其包含来自葡萄球菌蛋白A（SpA）的结构域C，或其功能片段或变体。 色谱配体具有在现场（CIP）条件下耐受苛刻清洁的有利能力，并且能够结合抗体的Fab片段。 配体可以具有末端偶联基团，例如精氨酸或半胱氨酸，以促进其与不溶性载体例如珠粒或膜的偶联。 本发明还涉及在分离抗体中使用配体的方法以及可以包括洗涤步骤和/或用碱再生的纯化方案。
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公开(公告)号：US09446329B2
公开(公告)日：2016-09-20
申请号：US12988553
申请日：2009-04-20
CPC分类号： B01D15/12 , B01D15/166 , B01D15/361 , G01N30/34 , G01N2030/347
摘要： A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hückel, wherein the ion size parameter a in the Debye-Hückel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device.
摘要翻译： 一种确定缓冲器中的至少一个的相对分量比例的方法; 酸或碱; 溶剂; 和任选的盐，用于提供预定pH和离子强度的液体混合物，其中相对组分比例使用Debye-Hückel等式确定，其中Debye-Hückel方程中的离子尺寸参数a被确定为 有助于液体混合物的离子强度的所有物质的加权平均离子尺寸，并且其中每种物质的离子强度被用作加权参数。 本方法也适用于提供液体混合物的方法。 此外，提供了一种缓冲液制备装置。
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6.发明授权Chromatography ligand comprising domain C from staphylococcus aureus protein A for antibody isolation 有权色谱配体包含来自金黄色葡萄球菌蛋白A的结构域C用于抗体分离公开(公告)号：US08772447B2
公开(公告)日：2014-07-08
申请号：US13559663
申请日：2012-07-27
申请人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
发明人： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC分类号： C07K17/00
CPC分类号： B01J20/24 , B01D15/3809 , B01J20/28019 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , B01J2220/54 , C07K1/22 , C07K14/31 , C07K16/00 , C07K17/00 , C07K17/10 , C07K2317/55
摘要： The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
摘要翻译： 本发明涉及一种色谱配体，其包含来自葡萄球菌蛋白A（SpA）的结构域C，或其功能片段或变体。 色谱配体具有在现场（CIP）条件下耐受苛刻清洁的有利能力，并且能够结合抗体的Fab片段。 配体可以具有末端偶联基团，例如精氨酸或半胱氨酸，以促进其与不溶性载体例如珠粒或膜的偶联。 本发明还涉及在分离抗体中使用配体的方法以及可以包括洗涤步骤和/或用碱再生的纯化方案。
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公开(公告)号：US10955092B2
公开(公告)日：2021-03-23
申请号：US13704270
申请日：2011-05-05
申请人： Catalin Andrei , Mikael Berg , Torbjorn Blank , Enrique Carredano , Karl Ekstrom , Tomas M. Karlsson , Jan-Erik Lynga , Roger Nordberg , Gustav Rodrigo , Henrik Sandegren
发明人： Catalin Andrei , Mikael Berg , Torbjorn Blank , Enrique Carredano , Karl Ekstrom , Tomas M. Karlsson , Jan-Erik Lynga , Roger Nordberg , Gustav Rodrigo , Henrik Sandegren
摘要： There is provided a method of preparing a mixed liquid having a first property and a second property. The method includes providing a first set of liquids, a second set of liquids, and a third set of liquids. The method includes combining the provided sets of liquids. Further, the method includes varying at least one of the liquid flows of the first and second sets and at least one liquid flow of the third set to adjust the first property and the second property to their respective predetermined values in the resulting mixed liquid flow.
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公开(公告)号：US20130274451A1
公开(公告)日：2013-10-17
申请号：US13996023
申请日：2011-12-19
申请人： Tomas Bjorkman , Elin Monie , Gustav Rodrigo
发明人： Tomas Bjorkman , Elin Monie , Gustav Rodrigo
IPC分类号： C07K1/22
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/745
摘要： The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine at the position corresponding to N28 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.
摘要翻译： 本发明涉及从液体中分离一种或多种含免疫球蛋白的蛋白质的方法。 该方法包括首先使液体与包含固定在载体上的配体的分离基质接触; 允许含有免疫球蛋白的蛋白质通过与配体的相互作用吸附到基质上; 随后是洗涤含有其上吸附有蛋白质的蛋白质的基质的任选步骤; 并通过使基质与释放蛋白质的洗脱液接触来回收所述含有免疫球蛋白的蛋白质。 该方法改进了以前的分离方法，因为每种配体包含蛋白A结构域（E，D，A，B，C）或蛋白质Z或其功能变体中的一种或多种，其中至少一种 在对应于蛋白A或蛋白Z的B结构域的N28的位置处取代天门冬酰胺的单体，并且其中与未取代的配体相比，配体提供了洗脱pH的增加。
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公开(公告)号：US20110039712A1
公开(公告)日：2011-02-17
申请号：US12988553
申请日：2009-04-20
CPC分类号： B01D15/12 , B01D15/166 , B01D15/361 , G01N30/34 , G01N2030/347
摘要： A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hückel, wherein the ion size parameter a in the Debye-Hückel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device.
摘要翻译： 一种确定缓冲器中的至少一个的相对分量比例的方法; 酸或碱; 溶剂; 和任选的盐，用于提供预定pH和离子强度的液体混合物，其中相对组分比例使用Debye-Hückel等式确定，其中Debye-Hückel方程中的离子尺寸参数a被确定为 有助于液体混合物的离子强度的所有物质的加权平均离子尺寸，并且其中每种物质的离子强度被用作加权参数。 本方法也适用于提供液体混合物的方法。 此外，提供了一种缓冲液制备装置。
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公开(公告)号：US09683013B2
公开(公告)日：2017-06-20
申请号：US13996023
申请日：2011-12-19
申请人： Tomas Bjorkman , Elin Monie , Gustav Rodrigo
发明人： Tomas Bjorkman , Elin Monie , Gustav Rodrigo
IPC分类号： B01D15/38 , B01J20/286 , C07K1/22 , B01J20/289 , C07K14/31 , C07K14/745 , B01J20/32
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/286 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K14/745
摘要： The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilized to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagine at the position corresponding to N28 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.
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