公开(公告)号：US07881517B2

公开(公告)日：2011-02-01

申请号：US11611934

申请日：2006-12-18

申请人： Erik Sloge ,  Tobias Soderman ,  Lennart Bjorkesten

发明人： Erik Sloge ,  Tobias Soderman ,  Lennart Bjorkesten

IPC分类号： G06K9/00

CPC分类号： G06K9/00127 ,  G06T7/0012

摘要： A method is provided for comparing multiple samples of cell extract containing a plurality of components. The method comprises the steps of preparing at least two samples of cell extract from at least two groups of cells and of exposing each of said sample of said cell extract to a different one of a set of matched markers, e.g. luminescent markers, to bind the marker to the cell extract to label the cell extract, each marker within said set of markers being capable of binding to the cell extract and can be individually detected from all other markers within said set. The samples are then mixed to form a mixture and said mixture is electrophoresed to separate the components within the cell extract. At least two electronic images of the electrophoresed mixture are obtained (I) by detection of the individual markers, each image being represented by detection of a marker different from the others. One resultant electronic image (Ires) of the obtained at least two electronic images is created (II) and analyzed in order to identify spot analysis areas (III). The identified spot analysis areas are applied on the respective at least two electronic images for evaluating said areas in order to detect spots representing components of said cell extracts (IV).

摘要翻译： 提供了一种用于比较包含多个组分的细胞提取物的多个样品的方法。 该方法包括以下步骤：从至少两组细胞制备细胞提取物的至少两个样品，并将每个所述细胞提取物的所述样品暴露于一组匹配的标记物，例如， 将标记物结合到细胞提取物以标记细胞提取物，所述标记物组内的每个标记物能够结合细胞提取物，并且可以从所述组中的所有其它标记物单独检测。 然后将样品混合以形成混合物，并将所述混合物电泳以分离细胞提取物内的组分。 通过检测各个标记获得电泳混合物的至少两个电子图像（I），每个图像通过检测与其他标记不同的标记来表示。 创建获得的至少两个电子图像的一个结果电子图像（Ires）（II）并进行分析以识别点分析区域（III）。 所识别的斑点分析区域被应用于相应的至少两个电子图像，用于评估所述区域，以便检测表示所述细胞提取物（IV）的组分的斑点。

公开(公告)号：US20070095757A1

公开(公告)日：2007-05-03

申请号：US10560728

申请日：2004-07-06

申请人： Anders Kaplan ,  Lennart Bjorkesten

发明人： Anders Kaplan ,  Lennart Bjorkesten

IPC分类号： B01D15/08

CPC分类号： G01N33/6848 ,  G01N30/7233 ,  G01N30/8675 ,  G01N30/8686 ,  G01N2030/045 ,  H01J49/0036

摘要： The method and measurement system according to the invention performs combined Chromatography and Mass Spectrometry analysis and comprises the steps of: performing an C/MS analysis 300; generating at least one first elution profile 305, wherein one dimension is an elution time of the chromatography, and one dimension is mass to charge ratio (m/z), and at least one dimension a signal intensity, and the signal from each biomolecule species is dispersed forming a plurality of signal peaks associated with each biomolecule species in the elution profile; and reassembling 310 the dispersed signal originating from one biomolecule species in the elution profile. The reassembling comprises an automated annotation adapted to reassemble signal variations in the elution profile that originate from the same biomolecule species and generating a biomolecule map. The automated annotating is simultaneously based on both the elution time-dimension and the m/z-dimension.

摘要翻译： 根据本发明的方法和测量系统进行组合色谱和质谱分析，包括以下步骤：进行C / MS分析300; 产生至少一个第一洗脱曲线305，其中一维是色谱的洗脱时间，一维是质荷比（m / z），至少一维的信号强度和来自每个生物分子物质的信号 分散形成与洗脱曲线中的每个生物分子物质相关联的多个信号峰; 并重新组装310来自洗脱曲线中的一个生物分子物质的分散信号。 重新组装包括适于重新组合来自相同生物分子物质的洗脱分布中的信号变化并产生生物分子图的自动注释。 自动注释同时基于洗脱时间尺寸和m / z维度。

公开(公告)号：US20110039712A1

公开(公告)日：2011-02-17

申请号：US12988553

申请日：2009-04-20

申请人： Lennart Bjorkesten ,  Enrique Carredano ,  Gunnar Malmquist ,  Gustav Rodrigo ,  Nils Stafstrom

发明人： Lennart Bjorkesten ,  Enrique Carredano ,  Gunnar Malmquist ,  Gustav Rodrigo ,  Nils Stafstrom

IPC分类号： B01D15/08 ,  B01D15/10 ,  C40B30/00 ,  G01N30/02 ,  G06F15/00

CPC分类号： B01D15/12 ,  B01D15/166 ,  B01D15/361 ,  G01N30/34 ,  G01N2030/347

摘要： A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hückel, wherein the ion size parameter a in the Debye-Hückel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device.

摘要翻译： 一种确定缓冲器中的至少一个的相对分量比例的方法; 酸或碱; 溶剂; 和任选的盐，用于提供预定pH和离子强度的液体混合物，其中相对组分比例使用Debye-Hückel等式确定，其中Debye-Hückel方程中的离子尺寸参数a被确定为 有助于液体混合物的离子强度的所有物质的加权平均离子尺寸，并且其中每种物质的离子强度被用作加权参数。 本方法也适用于提供液体混合物的方法。 此外，提供了一种缓冲液制备装置。

公开(公告)号：US10732176B2

公开(公告)日：2020-08-04

申请号：US14128379

申请日：2012-06-27

申请人： Lennart Bjorkesten ,  Sofia Edlund ,  Asa Hagner-McWhirter

发明人： Lennart Bjorkesten ,  Sofia Edlund ,  Asa Hagner-McWhirter

IPC分类号： G01N33/543 ,  G01N33/561 ,  G01N27/447

摘要： The present invention relates to a method for identification of specific target proteins in a protein sample following a detection procedure, such as a Western blotting procedure, wherein the membrane is probed with at least two primary antibodies directed against the same and/or different epitopes of the same target protein, and wherein specific binding to the target protein in a sample is differentiated from unspecific binding to the target protein by comparing the resulting sample patterns, such as bands or spot patterns, with each other.In a further step signals from the true target proteins are enhanced while signals resulting from unspecific binding are diminished.

公开(公告)号：US20140134752A1

公开(公告)日：2014-05-15

申请号：US14128379

申请日：2012-06-27

申请人： Lennart Bjorkesten ,  Sofia Edlund ,  Asa Hagner-McWhirter

发明人： Lennart Bjorkesten ,  Sofia Edlund ,  Asa Hagner-McWhirter

IPC分类号： G01N33/543

摘要： The present invention relates to a method for identification of specific target proteins in a protein sample following a detection procedure, such as a Western blotting procedure, wherein the membrane is probed with at least two primary antibodies directed against the same and/or different epitopes of the same target protein, and wherein specific binding to the target protein in a sample is differentiated from unspecific binding to the target protein by comparing the resulting sample patterns, such as bands or spot patterns, with each other.In a further step signals from the true target proteins are enhanced while signals resulting from unspecific binding are diminished.

摘要翻译： 本发明涉及在检测程序如蛋白质印迹法之后鉴定蛋白质样品中的特异性靶蛋白的方法，其中所述膜用至少两种针对相同和/或不同表位的一级抗体进行探针 相同的靶蛋白，并且其中通过将所得到的样品图案（例如条带或斑点图案）彼此比较，将样品中的目标蛋白质的特异性结合与靶向蛋白质的非特异性结合区分开。 在进一步的步骤中，来自真正靶蛋白的信号被增强，而来自非特异性结合的信号减弱。

公开(公告)号：US09446329B2

公开(公告)日：2016-09-20

申请号：US12988553

申请日：2009-04-20

申请人： Lennart Bjorkesten ,  Enrique Carredano ,  Gunnar Malmquist ,  Gustav Rodrigo ,  Nils Stafstrom

发明人： Lennart Bjorkesten ,  Enrique Carredano ,  Gunnar Malmquist ,  Gustav Rodrigo ,  Nils Stafstrom

IPC分类号： G01N30/34 ,  B01D15/12 ,  B01D15/16 ,  B01D15/36

CPC分类号： B01D15/12 ,  B01D15/166 ,  B01D15/361 ,  G01N30/34 ,  G01N2030/347

摘要： A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hückel, wherein the ion size parameter a in the Debye-Hückel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device.

摘要翻译： 一种确定缓冲器中的至少一个的相对分量比例的方法; 酸或碱; 溶剂; 和任选的盐，用于提供预定pH和离子强度的液体混合物，其中相对组分比例使用Debye-Hückel等式确定，其中Debye-Hückel方程中的离子尺寸参数a被确定为 有助于液体混合物的离子强度的所有物质的加权平均离子尺寸，并且其中每种物质的离子强度被用作加权参数。 本方法也适用于提供液体混合物的方法。 此外，提供了一种缓冲液制备装置。

公开(公告)号：US20070292044A1

公开(公告)日：2007-12-20

申请号：US11611934

申请日：2006-12-18

申请人： ERIK SLOGE ,  TOBIAS SODERMAN ,  LENNART BJORKESTEN

发明人： ERIK SLOGE ,  TOBIAS SODERMAN ,  LENNART BJORKESTEN

IPC分类号： G06K9/40

CPC分类号： G06K9/00127 ,  G06T7/0012

摘要： A method is provided for comparing multiple samples of cell extract containing a plurality of components. The method comprises the steps of preparing at least two samples of cell extract from at least two groups of cells and of exposing each of said sample of said cell extract to a different one of a set of matched markers, e.g. luminescent markers, to bind the marker to the cell extract to label the cell extract, each marker within said set of markers being capable of binding to the cell extract and can be individually detected from all other markers within said set. The samples are then mixed to form a mixture and said mixture is electrophoresed to separate the components within the cell extract. At least two electronic images of the electrophoresed mixture are obtained (I) by detection of the individual markers, each image being represented by detection of a marker different from the others. One resultant electronic image (Ires) of the obtained at least two electronic images is created (II) and analyzed in order to identify spot analysis areas (III). The identified spot analysis areas are applied on the respective at least two electronic images for evaluating said areas in order to detect spots representing components of said cell extracts (IV).

摘要翻译： 提供了一种用于比较包含多个组分的细胞提取物的多个样品的方法。 该方法包括以下步骤：从至少两组细胞制备细胞提取物的至少两个样品，并将每个所述细胞提取物的所述样品暴露于一组匹配的标记物，例如， 将标记物结合到细胞提取物以标记细胞提取物，所述标记物组内的每个标记物能够结合细胞提取物，并且可以从所述组中的所有其它标记物单独检测。 然后将样品混合以形成混合物，并将所述混合物电泳以分离细胞提取物内的组分。 通过检测各个标记获得电泳混合物的至少两个电子图像（I），每个图像通过检测与其他标记不同的标记来表示。 创建（II）获得的至少两个电子图像的一个结果电子图像（I），并分析以识别点分析区域（III）。 所识别的斑点分析区域被应用于相应的至少两个电子图像，用于评估所述区域，以便检测表示所述细胞提取物（IV）的组分的斑点。

公开(公告)号：US07155050B1

公开(公告)日：2006-12-26

申请号：US10129448

申请日：2000-10-31

申请人： Erik Sloge ,  Tobias Soderman ,  Lennart Bjorkesten

发明人： Erik Sloge ,  Tobias Soderman ,  Lennart Bjorkesten

IPC分类号： G06K9/00

CPC分类号： G06K9/00127 ,  G06T7/0012

摘要： A method is provided for comparing multiple samples of cell extract containing a plurality of components. The method comprises the steps of preparing at least two samples of cell extract from at least two groups of cells and of exposing each of said sample of said cell extract to a different one of a set of matched markers, e.g. luminescent markers, to bind the marker to the cell extract to label the cell extract, each marker within said set of markers being capable of binding to the cell extract and can be individually detected from all other markers within said set. The samples are then mixed to form a mixture and said mixture is electrophoresed to separate the components within the cell extract. At least two electronic images of the electrophoresed mixture are obtained (I) by detection of the individual markers, each image being represented by detection of a marker different from the others. One resultant electronic image (Ires) of the obtained at least two electronic images is created (II) and analyzed in order to identify spot analysis areas (III). The identified spot analysis areas are applied on the respective at least two electronic images for evaluating said areas in order to detect spots representing components of said cell extracts (IV).

摘要翻译： 提供了一种用于比较包含多个组分的细胞提取物的多个样品的方法。 该方法包括以下步骤：从至少两组细胞制备细胞提取物的至少两个样品，并将每个所述细胞提取物的所述样品暴露于一组匹配的标记物，例如， 将标记物结合到细胞提取物以标记细胞提取物，所述标记物组内的每个标记物能够结合细胞提取物，并且可以从所述组中的所有其它标记物单独检测。 然后将样品混合以形成混合物，并将所述混合物电泳以分离细胞提取物内的组分。 通过检测各个标记获得电泳混合物的至少两个电子图像（I），每个图像通过检测与其他标记不同的标记来表示。 创建（II）获得的至少两个电子图像的一个结果电子图像（I），并分析以识别点分析区域（III）。 所识别的斑点分析区域被应用于相应的至少两个电子图像，用于评估所述区域，以便检测表示所述细胞提取物（IV）的组分的斑点。