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    FLUIDICS DEVICE
    82.
    发明申请
    FLUIDICS DEVICE 有权
    流体装置

    公开(公告)号：US20120309644A1

    公开(公告)日：2012-12-06

    申请号：US13552356

    申请日：2012-07-18

    申请人： Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

    发明人： Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

    IPC分类号： C40B30/04 ,  G01N27/447 ,  B01D15/42 ,  F17D1/00

    CPC分类号： G01N30/461 ,  B01D15/325 ,  B01D15/362 ,  B01D15/363 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/563 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2400/0487 ,  C07K1/34 ,  G01N27/44704 ,  G01N27/44795 ,  G01N30/466 ,  G01N30/468 ,  Y10T137/2224 ,  Y10T137/794 ,  Y10T137/86187 ,  Y10T436/255

    摘要： The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.

    摘要翻译： 本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。 每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。 本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。 在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。

    Media For Membrane Ion Exchange Chromatography Based On Polymeric Primary Amines, Sorption Device Containing That Media, And Chromatography Scheme And Purification Method Using The Same
    85.
    发明申请
    Media For Membrane Ion Exchange Chromatography Based On Polymeric Primary Amines, Sorption Device Containing That Media, And Chromatography Scheme And Purification Method Using The Same 有权
    基于聚合一级胺的膜离子交换色谱介质，含有该介质的吸附装置和色谱方案及使用其的纯化方法

    公开(公告)号：US20120193278A1

    公开(公告)日：2012-08-02

    申请号：US13363397

    申请日：2012-02-01

    申请人： Mikhail Kozlov ,  Wilson Moya ,  Michael W. Phillips ,  Senthilkumar Ramaswamy ,  Brian Gagnon

    发明人： Mikhail Kozlov ,  Wilson Moya ,  Michael W. Phillips ,  Senthilkumar Ramaswamy ,  Brian Gagnon

    IPC分类号： B01D15/10

    CPC分类号： B01J20/28033 ,  B01D15/1871 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/3282 ,  B01J47/12 ,  Y10T428/249987

    摘要： Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities even at high conductivities and pH.

    摘要翻译： 介质和装置，例如包括这种介质的阴离子交换剂，其中介质是具有涂覆聚合物如聚烯丙基胺的表面的膜。 与基于季铵盐（包括三甲基铵配体）的常规配体相比，所得到的膜提供了更强的蛋白质杂质结合和优于宿主细胞蛋白质从生物样品中的去除。 还描述了用于纯化单克隆抗体的色谱方法和方法，其中阴离子交换吸附剂置于亲和柱（例如蛋白A或蛋白G亲和柱）的下游，以及任选的一种或多种抛光装置如阳离子交换柱。 阳离子交换池（或不使用阳离子交换剂的亲和柱交换池）的稀释很少或不需要，以降低样品的电导率。 即使在高电导率和pH下，吸附剂也能很好地结合宿主细胞蛋白质和其他杂质。

    Media For Membrane Ion Exchange Chromatography Based On Polymeric Primary Amines, Sorption Device Containing That Media, And Chromatography Scheme And Purification Method Using The Same
    86.
    发明申请
    Media For Membrane Ion Exchange Chromatography Based On Polymeric Primary Amines, Sorption Device Containing That Media, And Chromatography Scheme And Purification Method Using The Same 审中-公开
    基于聚合一级胺的膜离子交换色谱介质，含有该介质的吸附装置和色谱方案及使用其的纯化方法

    公开(公告)号：US20120121819A1

    公开(公告)日：2012-05-17

    申请号：US13339503

    申请日：2011-12-29

    申请人： Mikhail Kozlov ,  Wilson Moya ,  Michael W. Phillips ,  Senthilkumar Ramaswamy ,  Brian Gagnon

    发明人： Mikhail Kozlov ,  Wilson Moya ,  Michael W. Phillips ,  Senthilkumar Ramaswamy ,  Brian Gagnon

    IPC分类号： B05D5/00 ,  B05D3/06

    CPC分类号： B01J20/28033 ,  B01D15/1871 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/3282 ,  B01J47/12 ,  Y10T428/249987

    摘要： Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (e.g., Protein A or Protein G) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

    摘要翻译： 介质和装置，例如包括这种介质的阴离子交换剂，其中介质是具有涂覆聚合物如聚烯丙基胺的表面的膜。 与基于季铵盐（包括三甲基铵配体）的常规配体相比，所得到的膜提供了更强的蛋白质杂质结合和优于宿主细胞蛋白质从生物样品中的去除。 还描述了用于纯化单克隆抗体的色谱方案和方法，其中阴离子交换吸附剂置于亲和柱（例如蛋白A或蛋白G）和任选的一种或多种抛光装置如阳离子交换柱的下游。 阳离子交换池（或不使用阳离子交换剂的亲和柱交换池）的稀释很少或不需要，以降低样品的电导率。 即使在高电导率和pH下，吸附剂也能很好地结合生物样品中的宿主细胞蛋白质和其他杂质。

    Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
    87.
    发明授权
    Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same 有权
    用于基于聚合伯胺的膜离子交换色谱的介质，含有该介质的吸附装置和使用其的色谱方案和纯化方法

    公开(公告)号：US08137561B2

    公开(公告)日：2012-03-20

    申请号：US13167297

    申请日：2011-06-23

    申请人： Mikhail Kozlov ,  Wilson Moya ,  Michael W. Phillips ,  Senthilkumar Ramaswamy ,  Brian Gagnon

    发明人： Mikhail Kozlov ,  Wilson Moya ,  Michael W. Phillips ,  Senthilkumar Ramaswamy ,  Brian Gagnon

    IPC分类号： B01D15/08

    CPC分类号： B01J20/28033 ,  B01D15/1871 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/3282 ,  B01J47/12 ,  Y10T428/249987

    摘要： Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

    摘要翻译： 介质和装置，例如包括这种介质的阴离子交换剂，其中介质是具有涂覆聚合物如聚烯丙基胺的表面的膜。 与基于季铵盐（包括三甲基铵配体）的常规配体相比，所得到的膜提供了更强的蛋白质杂质结合和优于宿主细胞蛋白质从生物样品中的去除。 还描述了用于纯化单克隆抗体的色谱方法和方法，其中阴离子交换吸附剂置于亲和柱（例如蛋白A或蛋白G亲和柱）的下游，以及任选的一种或多种抛光装置如阳离子交换柱。 阳离子交换池（或不使用阳离子交换剂的亲和柱交换池）的稀释很少或不需要，以降低样品的电导率。 即使在高电导率和pH下，吸附剂也能很好地结合生物样品中的宿主细胞蛋白质和其他杂质。

    REMOVAL OF LIPOPOLYSACCHARIDES FROM PROTEIN-LIPOPOLYSACCHARIDE COMPLEXES BY NON FLAMMABLE SOLVENTS
    90.
    发明申请
    REMOVAL OF LIPOPOLYSACCHARIDES FROM PROTEIN-LIPOPOLYSACCHARIDE COMPLEXES BY NON FLAMMABLE SOLVENTS 有权
    通过非易燃溶剂从蛋白 - 脂多糖复合物中去除脂多糖

    公开(公告)号：US20110003976A1

    公开(公告)日：2011-01-06

    申请号：US12783319

    申请日：2010-05-19

    申请人： Philip Alfred Ropp ,  Michael Van Alen Murray

    发明人： Philip Alfred Ropp ,  Michael Van Alen Murray

    IPC分类号： C07K14/00

    CPC分类号： C07K1/22 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/426 ,  C07K1/18

    摘要： During the production of recombinant proteins from gram negative bacteria, lipopolysaccharides (LPS, endotoxin) are released along with the protein of interest. In many instances, LPS will copurify with the target protein due to specific or non-specific protein-ILPS interactions. We have investigated the ability of alkanediols to effect the separation of LPS from protein-LPS complexes while the complexes are immobilized on anion or cation exchange chromatographic media. Alkanediols provide a safer alternative to the use of other organics such as alcohols or acetonitrile due to their lower toxicity and decreased flammability. In addition, they are less costly than many of the detergents that have been used for such purposes. LPS removal efficiency increased with increasing alkane chain length. 1,2-alkanediols were more effective than terminal alkanediols in the separation of LPS from protein LPS complexes.

    摘要翻译： 在从革兰氏阴性细菌生产重组蛋白质期间，脂多糖（LPS，内毒素）与感兴趣的蛋白质一起释放。 在许多情况下，由于特异性或非特异性蛋白质 - ILPS相互作用，LPS将与目标蛋白质融合。 我们已经研究了链烷二醇在将蛋白质 - LPS复合物固定在阴离子或阳离子交换色谱介质上时能够分解LPS的能力。 由于其较低的毒性和较低的易燃性，烷烃二醇为其他有机物如醇或乙腈提供了更安全的替代方案。 此外，它们比用于这些目的的许多洗涤剂成本低。 LPS去除效率随着烷烃链长度的增加而增加。 在分离LPS与蛋白质LPS复合物时，1,2-链烷二醇比末端链烷二醇更有效。