公开(公告)号：US20160252434A1

公开(公告)日：2016-09-01

申请号：US15149383

申请日：2016-05-09

申请人： GENERAL ELECTRIC COMPANY

发明人： Reginald Donovan Smith ,  Patrick Joseph McCloskey

IPC分类号： G01N1/34

CPC分类号： G01N1/34 ,  C12N5/0087 ,  C12N2500/50 ,  G01N33/491

摘要： Provided herein are kit for providing a gamma sterilized aqueous dextran solution that increase the efficiency of blood separation by allowing the dextran solution to be sterilized by exposure to gamma radiation while maintaining sufficient molecular weight to act as a red blood cell aggregate. Also provided are methods of use.

摘要翻译： 本文提供了用于提供伽马灭菌的水性葡聚糖溶液的试剂盒，其通过使葡聚糖溶液通过暴露于γ辐射而消毒而提高血液分离的效率，同时保持足够的分子量用作红细胞聚集体。 还提供了使用方法。

公开(公告)号：US09429500B2

公开(公告)日：2016-08-30

申请号：US13781292

申请日：2013-02-28

申请人： Fluidigm Corporation

发明人： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A. A. West

IPC分类号： G01N1/34 ,  G01N1/28 ,  C12P19/34 ,  C12Q1/68 ,  G01N15/14 ,  B01L3/00 ,  B01L7/00

CPC分类号： G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

摘要： Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：US20160245834A1

公开(公告)日：2016-08-25

申请号：US14905218

申请日：2014-07-11

申请人： GENMAG BIOTECHNOLOGY CO., LTD.

发明人： Xinzhi Liu ,  Zhifang Guo ,  Haifeng Zhao

IPC分类号： G01N35/00 ,  C12N15/10 ,  G01N1/40

CPC分类号： G01N35/0098 ,  C12M33/06 ,  C12N15/1006 ,  G01N1/34 ,  G01N1/4077 ,  G01N2001/4038 ,  G01N2035/00465

摘要： The present invention provides an efficient apparatus for extracting biologically active substances through a magnetic particle method, comprising a magnetic needle, a magnetic needle moving mechanism, a sleeve and a sleeve moving mechanism and a vibration mechanism, a bracket, a positioning mechanism; the vibration mechanism is capable of generating vibrations to enable the sleeve moving mechanism to make upward and downward vibrations; wherein the magnetic needle can vertically insert into and move out of the sleeve; the sleeve can vertically insert into and move out of a sample treatment area; and the vibration mechanism comprises a lever bar, a movable fulcrum and a rotary mechanism. The present invention further provides an instrument comprising the apparatus for extracting biologically active substances. The present invention further provides a method for extracting and purifying biologically active substances using the apparatus or the instrument for extracting biologically active substances. The vibration frequency and amplitude of the apparatus and the instrument can be optionally adjusted, and the range of adjustments for the amplitude is greatly extended, so the apparatus and the instrument exhibit such advantages as making only small noise and have a long service life.

摘要翻译： 本发明提供一种通过磁性粒子法提取生物活性物质的有效装置，包括磁针，磁针移动机构，套筒和套筒移动机构以及振动机构，支架，定位机构; 振动机构能够产生振动，使得套筒移动机构能够产生向上和向下的振动; 其中所述磁针可以垂直地插入并移出所述套筒; 套筒可以垂直插入并移出样品处理区域; 振动机构包括杆杆，活动支点和旋转机构。 本发明还提供一种包括用于提取生物活性物质的装置的仪器。 本发明还提供使用该装置或仪器提取生物活性物质来提取和提纯生物活性物质的方法。 可以任意调节设备和仪器的振动频率和振幅，振幅调整范围大大延长，因此仪器和仪器具有噪声小，使用寿命长等优点。

公开(公告)号：US20160222373A1

公开(公告)日：2016-08-04

申请号：US14934951

申请日：2015-11-06

申请人： ZYMO RESEARCH CORPORATION

发明人： Xiyu JIA

IPC分类号： C12N15/10 ,  B01D15/38

CPC分类号： C12N15/101 ,  B01D15/22 ,  B01D15/3819 ,  B01L3/02 ,  B01L3/5021 ,  B01L2200/023 ,  B01L2300/0681 ,  C12N15/1017 ,  G01N1/34 ,  G01N1/405 ,  G01N30/6065 ,  G01N30/6069

摘要： A universal column is provided which allows purification methods utilizing centrifugation, syringe coupling and/or use of a vacuum source. Methods for using the universal column and kits comprising the universal column are described.

摘要翻译： 提供通用柱，其允许利用离心，注射器联接和/或使用真空源的纯化方法。 描述了使用通用色谱柱和包含通用色谱柱的试剂盒的方法。

公开(公告)号：US09395286B2

公开(公告)日：2016-07-19

申请号：US13977108

申请日：2011-12-30

申请人： Michael J. Cima ,  Gregory J. Ekchian ,  Christophoros C. Vassiliou ,  Vincent H. Liu ,  Christoph Wald ,  Sebastian Flacke ,  Fredric D. Gordon

发明人： Michael J. Cima ,  Gregory J. Ekchian ,  Christophoros C. Vassiliou ,  Vincent H. Liu ,  Christoph Wald ,  Sebastian Flacke ,  Fredric D. Gordon

IPC分类号： C12M1/34 ,  C12M1/00 ,  C12Q1/70 ,  G01N33/53 ,  G01N1/34 ,  A61F2/01 ,  G01N33/543

CPC分类号： G01N1/34 ,  A61F2/01 ,  G01N33/5302 ,  G01N33/5306 ,  G01N33/54313 ,  G01N33/56983

摘要： Trapping devices and methods are provided for capturing a medical analyte, in blood or another biological fluid. The device may include a structural substrate and a binding agent, such as an antibody, affixed to the structural substrate, wherein the binding agent is capable of binding or attaching with a medical analyte, such as a viral particle, and the device is configured for placement in a biological cavity or vessel (containing a biological fluid) in a patient. The trapping device, which may be in a twisted coil shape, is configured to trap at least some of the medical analyte, such a viral particle, present in the biological fluid. The method may include deploying a trapping device into the patient's blood vessel; after a period following the deployment, removing the trapping device from the biological cavity or blood vessel; and then analyzing the trapping device for the presence of the medical analyte.

摘要翻译： 提供捕获装置和方法用于捕获血液或其它生物流体中的医学分析物。 该装置可以包括结构底物和粘附到结构底物上的结合剂，例如抗体，其中结合剂能够与医学分析物例如病毒颗粒结合或附着，并且该装置被配置用于 放置在患者体内的生物腔或血管（含有生物流体）中。 可以处于扭曲线圈形状的捕获装置构造成捕获存在于生物流体中的至少一些医学分析物（例如病毒颗粒）。 该方法可以包括将捕获装置部署到患者的血管中; 在部署之后一段时间后，将捕获装置从生物腔或血管中取出; 然后分析捕获装置是否存在医用分析物。

公开(公告)号：US09380973B2

公开(公告)日：2016-07-05

申请号：US14251720

申请日：2014-04-14

申请人： Becton, Dickinson and Company

发明人： Gary D. Fletcher ,  Craig A. Gelfand ,  Bradley M. Wilkinson ,  Daniel J. Marchiarullo ,  Ashley Rachel Rothenberg

IPC分类号： A61B5/15 ,  G01N33/49 ,  A61M1/34 ,  G01N1/28 ,  G01N1/40 ,  G01N1/34 ,  B01L3/00 ,  B04B7/08 ,  A61B5/151 ,  A61B5/157

CPC分类号： A61B5/150213 ,  A61B5/1411 ,  A61B5/150022 ,  A61B5/150221 ,  A61B5/150267 ,  A61B5/150305 ,  A61B5/150343 ,  A61B5/150351 ,  A61B5/150412 ,  A61B5/150435 ,  A61B5/150442 ,  A61B5/150748 ,  A61B5/150755 ,  A61B5/150778 ,  A61B5/150969 ,  A61B5/151 ,  A61B5/15101 ,  A61B5/15105 ,  A61B5/15144 ,  A61B5/15198 ,  A61B5/157 ,  A61M1/34 ,  A61M1/36 ,  B01L3/502 ,  B01L3/5021 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2400/0478 ,  B04B7/08 ,  G01N1/28 ,  G01N1/34 ,  G01N1/4005 ,  G01N1/4077 ,  G01N33/491 ,  G01N2001/4016 ,  G01N2001/4088

摘要： A biological fluid sampling transfer device that is adapted to receive and separate a multi-component blood sample is disclosed. After separation, the biological fluid sampling transfer device is able to transfer a plasma portion of the blood sample to a point-of-care testing device. The biological fluid sampling transfer device of the present disclosure also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with an anticoagulant. The biological fluid sampling transfer device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid sampling transfer device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

摘要翻译： 公开了适于接收和分离多组分血液样本的生物流体取样转移装置。 分离后，生物液体取样转移装置能够将血液样品的血浆部分转移到护理点检测装置。 本公开的生物液体取样转移装置还提供了一种封闭的采样和转移系统，其减少血液样品的暴露并提供血液样品与抗凝血剂的快速混合。 生物液体取样转移装置可与血液检测装置接合，用于将血浆部分的一部分从生物流体取样转移装置封闭转移到血液检查装置。 血液检查装置适于接收血浆部分以分析血液样品并获得测试结果。

公开(公告)号：US20160184198A1

公开(公告)日：2016-06-30

申请号：US15061665

申请日：2016-03-04

申请人： BREATHALYZER EQUALIZER, LLC

发明人： McCoy Moretz

IPC分类号： A61K8/19 ,  A61Q11/00 ,  G01N1/34 ,  A61K8/97

CPC分类号： A61K8/19 ,  A61K8/97 ,  A61K8/9706 ,  A61K2800/5922 ,  A61Q11/00 ,  G01N1/34

摘要： There is provided a residual mouth alcohol inhibitor configured as an oral composition that absorbs residual mouth alcohol that remains in the mouth of an individual following the consumption of alcohol. The oral composition comprises a combination of sodium bicarbonate, calcium carbonate, parsley, kelp, fructose, citric acid, natural flavors, artificial flavors, maltodextrin, and silicon dioxide whereby the ratio of sodium bicarbonate to calcium bicarbonate in the composition is approximately 1:1 and the ratio of sodium bicarbonate to parsley in the composition is approximately 2:1 and the ratio of parsley to kelp in the composition is approximately 4:1.

摘要翻译： 提供了一种残留口腔酒精抑制剂，其配置为口服组合物，其吸收在消耗酒精后残留在个体口腔中的残留口酒。 口服组合物包含碳酸氢钠，碳酸钙，欧芹，海藻，果糖，柠檬酸，天然香料，人造香料，麦芽糖糊精和二氧化硅的组合，由此组合物中碳酸氢钠与碳酸氢钙的比例为约1:1 并且组合物中碳酸氢钠与欧芹的比例约为2:1，并且组合物中的欧芹和海带的比例约为4:1。

公开(公告)号：US09347087B2

公开(公告)日：2016-05-24

申请号：US14523764

申请日：2014-10-24

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Alexander Vlassov ,  Mu Li ,  Emily Zeringer ,  Richard Conrad

IPC分类号： C07K1/30 ,  C12Q1/68 ,  G01N1/34 ,  G01N33/50

CPC分类号： G01N1/34 ,  C12Q1/6806 ,  G01N33/5005 ,  G01N33/5076 ,  G01N33/92

摘要： Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

摘要翻译： 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 体积排除聚合物用于从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤来进行外来体分离。 还描述了沉淀后进一步分离外来体。

公开(公告)号：US20160139090A1

公开(公告)日：2016-05-19

申请号：US14898833

申请日：2014-06-19

申请人： UNIVERSITEIT LEIDEN

发明人： Jan-Williem SCHOONEN ,  Peter LINDENBURG ,  Paul VULTO ,  Thomas HANKEMEIER

IPC分类号： G01N30/12 ,  G01N30/72 ,  G01N30/06 ,  G01N30/64

CPC分类号： G01N30/12 ,  G01N1/28 ,  G01N1/34 ,  G01N1/4022 ,  G01N30/06 ,  G01N30/463 ,  G01N30/64 ,  G01N30/7233

摘要： The present invention relates to a droplet receiver, comprising a receptacle for receiving a droplet, a fluid conduit connected at a first end thereof to the droplet receptacle thereby providing a course within which the droplet received in the droplet receptacle moves, the conduit at its distal end in communication with a reservoir,and a fluid layer wetting the internal walls of the receptacle and conduit in such a way as permit reception of the droplet without loss of the received droplet due to wetting of the internal receptacle and conduit walls.

摘要翻译： 本发明涉及一种液滴接收器，包括用于接收液滴的容器，在其第一端处连接到液滴容器的流体导管，从而提供容纳在液滴容器中的液滴移动的过程，其远端处的导管 与储存器连通，并且液体层以允许接收液滴而不会由于内部容器和导管壁的润湿而损失所接收的液滴的方式润湿容器和导管的内壁。

公开(公告)号：US20160116383A1

公开(公告)日：2016-04-28

申请号：US14922254

申请日：2015-10-26

申请人： Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG

发明人： Ralf Bernhard ,  Joachim Albert ,  Maike Haslauer

IPC分类号： G01N1/34 ,  C02F1/50 ,  B01D35/02 ,  G01N21/78 ,  G01N1/44 ,  G01N21/59 ,  G01J1/42 ,  C02F1/32 ,  G01N1/10

CPC分类号： G01N1/34 ,  C02F1/325 ,  C02F1/505 ,  G01J1/42 ,  G01N1/10 ,  G01N1/44 ,  G01N21/59 ,  G01N21/78 ,  G01N2021/7783 ,  G01N2201/12

摘要： A sample collection apparatus, especially for an analyzer to determine a measurand of a liquid, comprising: a sample collection unit connected to a liquid removal point via a fluid line, a feed device that is designed to transport liquids from the liquid removal point through the fluid line to the sample collection unit, and a filter unit arranged in the flow path from the liquid removal point through the fluid line to the sample collection unit, characterized in that the sample collection apparatus comprises at least one surface or a device effecting a reduction of germs in the liquid transported through the flow path from the filter unit to the sample collection unit.

摘要翻译： 一种样品收集装置，特别是用于确定液体被测量的分析仪，包括：通过流体管线连接到液体去除点的样品收集单元，被设计成将液体从液体移除点输送到 流体管线到样品收集单元，以及过滤器单元，布置在从液体移除点通过流体管线到样品收集单元的流动路径中，其特征在于，样品收集装置包括至少一个表面或实现还原的装置 在通过从过滤器单元到样品收集单元的流动路径输送的液体中的细菌。