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    AMPLIFICATION PRIMERS AND METHODS
    61.
    发明申请
    AMPLIFICATION PRIMERS AND METHODS 审中-公开
    放大倍数和方法

    公开(公告)号：US20150329855A1

    公开(公告)日：2015-11-19

    申请号：US14367781

    申请日：2012-12-21

    Applicant: IBIS BIOSCIENCES, INC.

    Inventor： Phillip N. Gray ,  Mark W. Eshoo

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1096 ,  C12P19/34 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q2525/131 ,  C12Q2525/151 ,  C12Q2525/161 ,  C12Q2525/179

    Abstract: The present invention provides methods, compositions, and kits for performing amplification (e.g., whole genome amplification) employing primers that have a 5′ restriction site, a 3′ random sequence (e.g., a random hexamer), and an identifiable barcode sequence. In certain embodiments, the amplification generates individual amplified sequenced that are ligated together to form concatamers containing at least two amplified sequences (e.g., not contiguous on the original target sequence) that are separated by the barcode sequences. In particular embodiments, a plurality of the concatamers are sequenced and aligned with an alignment algorithm that uses the barcode sequences to identify artificial junctions between amplified sequences.

    Abstract translation: 本发明提供了使用具有5'限制性位点，3'随机序列（例如，随机六聚体）和可识别条形码序列的引物进行扩增（例如全基因组扩增）的方法，组合物和试剂盒。 在某些实施方案中，扩增产生连接在一起的单个扩增测序，以形成包含由条形码序列分离的至少两个扩增序列（例如，在原始靶序列上不连续）的连接体。 在特定实施例中，多个并置器被排序并与使用条形码序列来识别扩增序列之间的人工连接的对准算法对准。

    METHODS FOR ADDING ADAPTERS TO NUCLEIC ACIDS AND COMPOSITIONS FOR PRACTICING THE SAME
    63.
    发明申请
    METHODS FOR ADDING ADAPTERS TO NUCLEIC ACIDS AND COMPOSITIONS FOR PRACTICING THE SAME 有权
    将适配剂加入到核酸中的方法和用于实施其的组合物

    公开(公告)号：US20150203906A1

    公开(公告)日：2015-07-23

    申请号：US14566445

    申请日：2014-12-10

    Applicant: CLONTECH LABORATORIES, INC.

    Inventor： Craig Betts ,  Andrew Alan Farmer

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12N15/1096 ,  C12Q2525/191

    Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template nucleic acid, a template switch oligonucleotide, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template nucleic acid and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid including a region polymerized from the dNTPs by the polymerase. The methods further include attaching sequencing platform adapter constructs to ends of the product nucleic acid or a derivative thereof. Aspects of the invention further include compositions and kits.

    Abstract translation: 提供了向核酸加入衔接子的方法。 所述方法包括在反应混合物中结合模板核酸，模板切换寡核苷酸，聚合酶和dNTP。 反应混合物组分在足以产生包含模板核酸和模板切换寡核苷酸的产物核酸的条件下进行组合，每个核酸与包含通过聚合酶从dNTP聚合的区域的单一产物核酸的相邻区域杂交。 所述方法还包括将测序平台衔接子构建体连接到产物核酸的末端或其衍生物。 本发明的方面还包括组合物和试剂盒。

    METHODS OF CONSTRUCTING SMALL RNA LIBRARIES AND THEIR USE FOR EXPRESSION PROFILING OF TARGET RNAS
    65.
    发明申请
    METHODS OF CONSTRUCTING SMALL RNA LIBRARIES AND THEIR USE FOR EXPRESSION PROFILING OF TARGET RNAS 有权
    构建小RNA文库的方法及其用于目标RNA的表达谱分析

    公开(公告)号：US20150051099A1

    公开(公告)日：2015-02-19

    申请号：US14367200

    申请日：2012-12-21

    Applicant: SomaGenics, Inc.

    Inventor： Sergei A. Kazakov

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12Q1/6837 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6813 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2525/207 ,  C12Q2539/101 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2525/307

    Abstract: Methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) are disclosed herein. Methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) can be used to attach adapters and/or linkers to target RNAs. Methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) can be used in reactions, including, but not limited to, ligation reactions, amplification reactions, and sequencing reactions. Additionally, methods, compositions, and kits comprising target-specific oligonucleotides (TSOs) can be used for reducing and/or preventing the formation of secondary structures in target RNAs. These methods, compositions, and kits can also find use in a number of applications, for example, any application that benefits from stabilizing primary RNA structure, such as detecting and quantifying target RNAs in a sample, in the construction of small RNA libraries, in microarray and RT-qPCR applications, etc.

    Abstract translation: 本文公开了包含靶特异性寡核苷酸（TSO）的方法，组合物和试剂盒。 包含靶特异性寡核苷酸（TSO）的方法，组合物和试剂盒可用于将衔接子和/或连接体连接至靶RNA。 包括靶特异性寡核苷酸（TSO）的方法，组合物和试剂盒可用于反应，包括但不限于连接反应，扩增反应和测序反应。 另外，包含靶特异性寡核苷酸（TSO）的方法，组合物和试剂盒可用于减少和/或预防靶RNA中二级结构的形成。 这些方法，组合物和试剂盒也可用于许多应用，例如从稳定初级RNA结构中获益的任何应用，例如检测和定量样品中的靶RNA，构建小RNA文库 微阵列和RT-qPCR应用等

    METHODS AND COMPOSITIONS FOR PREPARING RNA FROM A FIXED SAMPLE
    68.
    发明申请
    METHODS AND COMPOSITIONS FOR PREPARING RNA FROM A FIXED SAMPLE 审中-公开
    从固定样品制备RNA的方法和组合物

    公开(公告)号：US20140329703A1

    公开(公告)日：2014-11-06

    申请号：US14206642

    申请日：2014-03-12

    Applicant: Applied Biosystem, LLC

    Inventor： Richard CONRAD ,  Emily Zeringer

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12N15/1003 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/686

    Abstract: The present invention provides improved methods and compositions for RNA isolation. In particular embodiments the present invention concerns the use of methods and compositions for the isolation of full-length RNA from fixed tissue samples. The present invention provides methods for digesting and extracting RNA from a fixed tissue sample.

    Abstract translation: 本发明提供用于RNA分离的改进的方法和组合物。 在具体实施方案中，本发明涉及用于从固定组织样品分离全长RNA的方法和组合物的用途。 本发明提供从固定组织样品中消化和提取RNA的方法。

    cDNA Synthesis Improvements
    70.
    发明申请
    cDNA Synthesis Improvements 审中-公开
    cDNA合成改进

    公开(公告)号：US20140295502A1

    公开(公告)日：2014-10-02

    申请号：US14276944

    申请日：2014-05-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Wu-Bo LI ,  Joel Jessee ,  Christian Gruber

    IPC: C12P19/34 ,  C07K16/40

    CPC classification number: C12P19/34 ,  C07K16/40 ,  C12N15/1096

    Abstract: The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.

    Abstract translation: 本发明一般涉及制备cDNA分子和cDNA文库的方法。 本发明还涉及根据这些方法产生的cDNA分子和cDNA文库，以及含有此类cDNA分子和文库的载体和宿主细胞。 本发明还涉及用于制备本发明的cDNA分子和文库的试剂盒。

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