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    Micro-chamber processing apparatus for cell culture and method
    31.
    发明授权
    Micro-chamber processing apparatus for cell culture and method 有权
    用于细胞培养和方法的微室处理装置

    公开(公告)号：US08247222B2

    公开(公告)日：2012-08-21

    申请号：US13036600

    申请日：2011-02-28

    Applicant: Akihiro Hattori ,  Kenji Yasuda

    Inventor： Akihiro Hattori ,  Kenji Yasuda

    IPC: C12M1/00 ,  C12M1/34

    CPC classification number: C12M23/16 ,  B01J2219/00317 ,  B01J2219/00441 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/163

    Abstract: This invention relates to a method and apparatus for processing cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, the absorption layer having absorbency in visible and infrared regions, and the gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and the light source having a monochromatic light in the specific wave length, wherein the light source is disposed such that it irradiates on the absorption layer and/or the gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.

    Abstract translation: 本发明涉及一种用于处理细胞培养物的方法和装置。 该装置包括一个微室，该微室包括不超过一个吸收层和至少一个凝胶层，依次层叠在透明基板上，在可见光和红外区域中没有明显的吸收性，以及至少一个光源，吸收 在可见光和红外区域具有吸收性的层，凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物，在室温下处于凝胶状态，具有特定的吸光度 可见光和红外区域的波长，以及具有特定波长的单色光的光源，其中光源被设置成使其照射在吸收层和/或凝胶层上，除了当没有吸收 在透明基板上层叠由凝胶状材料构成的至少两层。

    Cell separation chip and cell culturing method using the same
    33.
    发明申请
    Cell separation chip and cell culturing method using the same 审中-公开
    细胞分离芯片和细胞培养方法使用相同

    公开(公告)号：US20080003678A1

    公开(公告)日：2008-01-03

    申请号：US11819681

    申请日：2007-06-28

    Applicant: Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

    Inventor： Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

    IPC: C12N5/06 ,  C12M1/00

    CPC classification number: B01L3/502761 ,  B01L3/502753 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  C12M45/00 ,  C12M47/04 ,  G01N15/1031 ,  G01N15/12 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N2015/1081 ,  G01N2015/149

    Abstract: An inexpensive cell analysis and separation apparatus using a flow channel formed on one surface of a substrate and a chip replaceable for each sample, and a method for culturing the separated cells without contamination, are provided. A flow channel for allowing a cell-containing buffer solution to flow is provided. Cells are detected in the middle of the flow channel, and separated to a plurality of downstream flow channels based on whether each cell fulfills a predetermined condition. A culturing tank for collecting the condition-fulfilling cells is covered with a semipermeable membrane at a top surface so as to prevent contamination during cell separation. When the cell separation is finished, the flow channel communicated with the culturing tank accommodating the condition-fulfilling cells is closed, and the culturing tank is cut off from the apparatus and put into a culturing device containing a predetermined medium to culture the cells.

    Abstract translation: 提供了使用形成在基板的一个表面上的流动通道和可替代每个样品的芯片的便宜的电池分析和分离装置，以及用于培养分离的电池而不被污染的方法。 提供了允许含细胞的缓冲溶液流动的流动通道。 基于每个小区是否满足预定条件，在流信道的中间检测小区，并将其分离成多个下游流信道。 用于收集状态满足细胞的培养槽在顶表面被半透膜覆盖，以防止细胞分离过程中的污染。 当细胞分离完成时，与容纳条件满足细胞的培养箱连通的流动通道关闭，并将培养箱从装置中切下并放入含有预定培养基的培养装置中以培养细胞。

    Method of inspecting a DNA chip
    34.
    发明授权
    Method of inspecting a DNA chip 有权
    检查DNA芯片的方法

    公开(公告)号：US07217573B1

    公开(公告)日：2007-05-15

    申请号：US09678652

    申请日：2000-10-04

    Applicant: Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

    Inventor： Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

    IPC: G01N33/00 ,  G01N33/53 ,  C12Q1/68 ,  C12M3/00 ,  C07H21/04 ,  H01J3/14

    CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  Y10S436/80 ,  Y10T436/143333

    Abstract: A method of inspecting a DNA chip and an apparatus therefor that allow a picture to be reconstructed in the following steps: A plurality of irradiation spots are formed on a DNA probe array mounted on a stage. Then, the stage is displaced in X, Y directions so as to execute a scanning, thereby irradiating substantially all the entire surface of the DNA probe array. Next, a plurality of emitted fluorescent lights, which are generated from the plurality of irradiation spot portions on the DNA probe array, are converged and are then detected simultaneously by multi detectors. Finally, a data processing apparatus processes the detected signals, thereby reconstructing the picture.

    Abstract translation: 一种用于检查DNA芯片的方法及其装置，其允许以下列步骤重建图像：在安装在平台上的DNA探针阵列上形成多个照射点。 然后，在X，Y方向上移位台，以进行扫描，从而基本上照射DNA探针阵列的整个表面。 接下来，从DNA探针阵列上的多个照射点部分产生的多个发射荧光灯被会聚，然后由多个检测器同时检测。 最后，数据处理装置处理检测到的信号，从而重建图像。

    Cell measuring and sorting chip
    35.
    发明申请
    Cell measuring and sorting chip 有权
    电池测量和分选芯片

    公开(公告)号：US20060194307A1

    公开(公告)日：2006-08-31

    申请号：US11317004

    申请日：2005-12-27

    Applicant: Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    Inventor： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    IPC: C12M1/34 ,  B07C5/00

    CPC classification number: G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

    Abstract: The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow a buffer fluid not containing cells to flow down from both sides of the first flow path; a fourth flow path which allows the buffer fluid as a single flow path formed by the join of the buffer fluid in the first flow path and the buffer fluid in the second and third flow paths; a cell detecting region for detecting cells flowing with a buffer fluid down the fourth flow path; a cell sorting region for sorting the cells according to a type of the cells detected. The first flow path, the second and the third flow paths, the fourth flow path, the cell detecting region and the cell sorting region provided in a cascade. Two flow paths are provided to allow the cells sorted to flow downstream of the cell sorting region. The buffer fluid flowing in the first to the fourth flow paths is supplied from reservoirs having the same fluid level. The first flow path and the fourth flow path are structured to have substantially the same width or cross-section area.

    Abstract translation: 本发明提供一种使用廉价的芯片的细胞分选装置，其能够用于每个样品进行细胞分选。 细胞分选装置使用形成在基板上的流路，具有以下结构。 芯片包括：允许包含电池的缓冲流体向下流动的第一流路; 第二和第三流动路径，其将第一流动路径放置在其间并允许不含有电池的缓冲流体从第一流动路径的两侧向下流动; 第四流动路径，其允许缓冲流体作为由第一流动路径中的缓冲流体的连接和第二和第三流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测在第四流路下方以缓冲液流动的细胞; 用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一流路，第二流路，第三流路，第四流路，单元检测区域和电池排列区域。 提供了两个流动路径以允许排序的细胞流动到细胞分选区域的下游。 在第一至第四流动路径中流动的缓冲流体从具有相同流体液位的储存器供应。 第一流路和第四流路被构造成具有基本相同的宽度或横截面面积。

    Apparatus and method for machining microchamber for cell culture
    36.
    发明申请
    Apparatus and method for machining microchamber for cell culture 有权
    用于细胞培养微孔加工的装置和方法

    公开(公告)号：US20060141619A1

    公开(公告)日：2006-06-29

    申请号：US10544799

    申请日：2004-02-19

    Applicant: Akihiro Hattori ,  Kenji Yasuda

    Inventor： Akihiro Hattori ,  Kenji Yasuda

    IPC: C12N5/06 ,  C12M1/00

    CPC classification number: C12M23/16 ,  B01J2219/00317 ,  B01J2219/00441 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/163

    Abstract: There is provided a processing apparatus and a processing method for micro-chamber for cell culture having a desired culturing space. The micro-chamber processing apparatus for cell culture of the present invention comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, said absorption layer having absorbency in visible and infrared regions, and said gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and said light source having a monochromatic light in said specific wave length, wherein said light source is disposed such that it irradiates on said absorption layer and/or said gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on said transparent base plate.

    Abstract translation: 提供了具有期望的培养空间的用于细胞培养的微室的处理装置和处理方法。 本发明的细胞培养用微细胞处理装置，具有不超过一个吸收层和至少一层凝胶层的微室，依次层叠在透明基板上，在可见光和红外线中不具有明显的吸收性 区域和至少一个光源，所述吸收层在可见光和红外区域具有吸收性，所述凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物 在具有特定波长的可见光和红外区域具有吸收能力，并且所述光源具有所述特定波长的单色光，其中所述光源被设置成使得其照射在所述吸收层和/或 所述凝胶层除了没有设置吸收层之外，将由凝胶状材料构成的至少两层层合在所述透明体b上 灰板

    Substrate holding apparatus and substrate process system
    39.
    发明授权
    Substrate holding apparatus and substrate process system 有权
    基板保持装置和基板处理系统

    公开(公告)号：US06168669A

    公开(公告)日：2001-01-02

    申请号：US09250394

    申请日：1999-02-16

    Applicant: Kenji Yasuda ,  Naruaki Iida

    Inventor： Kenji Yasuda ,  Naruaki Iida

    IPC: C23C1600

    CPC classification number: H01L21/68707

    Abstract: Supporting members are provided at three places, for example, on a frame section of a pair of tweezers. Each supporting member has a tapered face as an inclined guide for allowing a rim portion of a substrate to slide down to be guided to a predetermined position. A vertical wall which is formed continuing from the tapered face and being nearly perpendicular to a supported face of a wafer is provided at an upper end of the tapered face. Even when the rim portion of the wafer rises along the tapered face with the movement of the tweezers, the rim portion stops by hitting against the vertical wall, thereby preventing the wafer from falling from the tweezers.

    Abstract translation: 支撑构件设置在三个位置，例如，在一对镊子的框架部分上。 每个支撑构件具有锥形面作为倾斜引导件，用于允许基板的边缘部分向下滑动以被引导到预定位置。 在锥面的上端设置有从锥形表面连续并且几乎垂直于晶片的支撑面的垂直壁。 即使当晶片的边缘部分随着镊子的移动而沿着锥形表面上升时，边缘部分通过撞击垂直壁而停止，从而防止晶片从镊子落下。

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