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    Cell measuring and sorting chip
    8.
    发明授权
    Cell measuring and sorting chip 有权
    电池测量和分选芯片

    公开(公告)号：US08361412B2

    公开(公告)日：2013-01-29

    申请号：US11317004

    申请日：2005-12-27

    Applicant: Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    Inventor： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    IPC: B01L3/00

    CPC classification number: G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

    Abstract: A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.

    Abstract translation: 一种使用廉价芯片的细胞分选装置，能够对每个样品进行交换。 芯片包括：允许包含电池的缓冲流体向下流动的第一流动路径; 第二和第三流动路径，其中第一流动路径在其间并且允许不包含电池的缓冲流体向下流动; 第四流路，其允许缓冲流体作为通过连接其它三个流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测沿着第四流动路径流动的缓冲液的细胞; 以及用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一至第四流动路径级联，从具有相同流体水平的储存器提供缓冲流体，并且具有基本上相同的宽度或横截面面积。

    Cell measuring and sorting chip
    9.
    发明申请
    Cell measuring and sorting chip 有权
    电池测量和分选芯片

    公开(公告)号：US20060194307A1

    公开(公告)日：2006-08-31

    申请号：US11317004

    申请日：2005-12-27

    Applicant: Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    Inventor： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    IPC: C12M1/34 ,  B07C5/00

    CPC classification number: G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

    Abstract: The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow a buffer fluid not containing cells to flow down from both sides of the first flow path; a fourth flow path which allows the buffer fluid as a single flow path formed by the join of the buffer fluid in the first flow path and the buffer fluid in the second and third flow paths; a cell detecting region for detecting cells flowing with a buffer fluid down the fourth flow path; a cell sorting region for sorting the cells according to a type of the cells detected. The first flow path, the second and the third flow paths, the fourth flow path, the cell detecting region and the cell sorting region provided in a cascade. Two flow paths are provided to allow the cells sorted to flow downstream of the cell sorting region. The buffer fluid flowing in the first to the fourth flow paths is supplied from reservoirs having the same fluid level. The first flow path and the fourth flow path are structured to have substantially the same width or cross-section area.

    Abstract translation: 本发明提供一种使用廉价的芯片的细胞分选装置，其能够用于每个样品进行细胞分选。 细胞分选装置使用形成在基板上的流路，具有以下结构。 芯片包括：允许包含电池的缓冲流体向下流动的第一流路; 第二和第三流动路径，其将第一流动路径放置在其间并允许不含有电池的缓冲流体从第一流动路径的两侧向下流动; 第四流动路径，其允许缓冲流体作为由第一流动路径中的缓冲流体的连接和第二和第三流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测在第四流路下方以缓冲液流动的细胞; 用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一流路，第二流路，第三流路，第四流路，单元检测区域和电池排列区域。 提供了两个流动路径以允许排序的细胞流动到细胞分选区域的下游。 在第一至第四流动路径中流动的缓冲流体从具有相同流体液位的储存器供应。 第一流路和第四流路被构造成具有基本相同的宽度或横截面面积。

    CELLOMICS SYSTEM
    10.
    发明申请
    CELLOMICS SYSTEM 审中-公开

    公开(公告)号：US20090042739A1

    公开(公告)日：2009-02-12

    申请号：US12143181

    申请日：2008-06-20

    Applicant: Kazunori Okano ,  Kenji Yasuda

    Inventor： Kazunori Okano ,  Kenji Yasuda

    IPC: C40B30/10 ,  C40B60/12 ,  C40B40/02

    CPC classification number: B01L7/50 ,  A01N1/0284 ,  G01N33/5061 ,  G01N33/566

    Abstract: In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.

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