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公开(公告)号：US08361412B2
公开(公告)日：2013-01-29
申请号：US11317004
申请日：2005-12-27
Applicant: Kenji Yasuda , Akihiro Hattori , Kazunori Okano
Inventor： Kenji Yasuda , Akihiro Hattori , Kazunori Okano
IPC: B01L3/00
CPC classification number: G01N15/147 , B01L3/502761 , B01L2200/0647 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2400/0415 , B01L2400/0487 , C12M47/04 , G01N35/085 , G01N2015/149 , G06T7/0012 , G06T2207/10056 , G06T2207/30024
Abstract: A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.
Abstract translation: 一种使用廉价芯片的细胞分选装置，能够对每个样品进行交换。 芯片包括：允许包含电池的缓冲流体向下流动的第一流动路径; 第二和第三流动路径，其中第一流动路径在其间并且允许不包含电池的缓冲流体向下流动; 第四流路，其允许缓冲流体作为通过连接其它三个流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测沿着第四流动路径流动的缓冲液的细胞; 以及用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一至第四流动路径级联，从具有相同流体水平的储存器提供缓冲流体，并且具有基本上相同的宽度或横截面面积。
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公开(公告)号：US20060194307A1
公开(公告)日：2006-08-31
申请号：US11317004
申请日：2005-12-27
Applicant: Kenji Yasuda , Akihiro Hattori , Kazunori Okano
Inventor： Kenji Yasuda , Akihiro Hattori , Kazunori Okano
CPC classification number: G01N15/147 , B01L3/502761 , B01L2200/0647 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2400/0415 , B01L2400/0487 , C12M47/04 , G01N35/085 , G01N2015/149 , G06T7/0012 , G06T2207/10056 , G06T2207/30024
Abstract: The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow a buffer fluid not containing cells to flow down from both sides of the first flow path; a fourth flow path which allows the buffer fluid as a single flow path formed by the join of the buffer fluid in the first flow path and the buffer fluid in the second and third flow paths; a cell detecting region for detecting cells flowing with a buffer fluid down the fourth flow path; a cell sorting region for sorting the cells according to a type of the cells detected. The first flow path, the second and the third flow paths, the fourth flow path, the cell detecting region and the cell sorting region provided in a cascade. Two flow paths are provided to allow the cells sorted to flow downstream of the cell sorting region. The buffer fluid flowing in the first to the fourth flow paths is supplied from reservoirs having the same fluid level. The first flow path and the fourth flow path are structured to have substantially the same width or cross-section area.
Abstract translation: 本发明提供一种使用廉价的芯片的细胞分选装置，其能够用于每个样品进行细胞分选。 细胞分选装置使用形成在基板上的流路，具有以下结构。 芯片包括：允许包含电池的缓冲流体向下流动的第一流路; 第二和第三流动路径，其将第一流动路径放置在其间并允许不含有电池的缓冲流体从第一流动路径的两侧向下流动; 第四流动路径，其允许缓冲流体作为由第一流动路径中的缓冲流体的连接和第二和第三流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测在第四流路下方以缓冲液流动的细胞; 用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一流路，第二流路，第三流路，第四流路，单元检测区域和电池排列区域。 提供了两个流动路径以允许排序的细胞流动到细胞分选区域的下游。 在第一至第四流动路径中流动的缓冲流体从具有相同流体液位的储存器供应。 第一流路和第四流路被构造成具有基本相同的宽度或横截面面积。
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公开(公告)号：US20060177348A1
公开(公告)日：2006-08-10
申请号：US11348361
申请日：2006-02-07
Applicant: Kenji Yasuda , Akihiro Hattori , Kazunori Okano
Inventor： Kenji Yasuda , Akihiro Hattori , Kazunori Okano
IPC: G01N33/48
CPC classification number: B01L3/502761 , B01L3/502715 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0421 , B01L2400/0487 , G01N15/147 , G01N2015/149
Abstract: A cell sorting chip and a cell sorting technology are to be established which can positively detect and sort a specified cell for cell separation and detection using micro flow paths formed on a substrate, whereby a cell analyzing/sorting device is provided which uses an inexpensive disposable chip replaceable for each sample. To this end, micro flow paths formed on the substrate are formed, and cells are roughly sorted in a first stage and then finely sorted in a second stage. More specifically, cells are sorted roughly by using scattered light or according to intensity of luminescence in the first stage. In the second stage, the roughly sorted cells are sorted with high precision using image recognition.
Abstract translation: 建立细胞分选芯片和细胞分选技术，其可以使用形成在基底上的微流路来积极地检测和分类用于细胞分离和检测的细胞，由此提供细胞分析/分选装置，其使用便宜的一次性 芯片可替代每个样品。 为此，形成在基板上形成的微流路，在第一阶段细胞粗略分选，然后在第二阶段细分。 更具体地，通过使用散射光或根据第一阶段中的发光强度粗略地对细胞进行分类。 在第二阶段，使用图像识别以高精度对大致排序的细胞进行排序。
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公开(公告)号：US20110212522A1
公开(公告)日：2011-09-01
申请号：US13036600
申请日：2011-02-28
Applicant: Akihiro Hattori , Kenji Yasuda
Inventor： Akihiro Hattori , Kenji Yasuda
CPC classification number: C12M23/16 , B01J2219/00317 , B01J2219/00441 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/0063 , B01J2219/00637 , B01J2219/00743 , B01L3/5085 , B01L2200/12 , B01L2300/0819 , B01L2300/12 , B01L2300/163
Abstract: This invention relates to a method and apparatus for process cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, said absorption layer having absorbency in visible and infrared regions, and said gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and said light source having a monochromatic light in said specific wave length, wherein said light source is disposed such that it irradiates on said absorption layer and/or said gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on said transparent base plate.
Abstract translation: 本发明涉及一种用于过程细胞培养的方法和装置。 该装置包括一个微室，该微室包括不超过一个吸收层和至少一个凝胶层，依次层叠在透明基板上，在可见光和红外区域中没有明显的吸收性，以及至少一个光源，所述吸收 并且所述凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物，在室温下处于凝胶状态，具有特定的吸光度 可见光和红外区域的波长，以及所述光源具有所述特定波长的单色光，其中所述光源被设置成使其照射在所述吸收层和/或所述凝胶层上，除了当没有吸收 在所述透明基板上层压由凝胶状材料构成的至少两层。
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公开(公告)号：US09109197B2
公开(公告)日：2015-08-18
申请号：US13138785
申请日：2010-03-31
Applicant: Kenji Yasuda , Masahito Hayashi , Akihiro Hattori
Inventor： Kenji Yasuda , Masahito Hayashi , Akihiro Hattori
CPC classification number: C12M47/04 , B01D21/283 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2400/0415 , B01L2400/0424 , B01L2400/0439
Abstract: Provided is a device for concentrating and separating cells, which has a function for continuously concentrating cells; a function for then continuously arranging the concentrated cells in predetermined regions of a flow path; a function for simultaneously identifying shape and fluorescent emission in one-cell units on the basis of cell concentration and purification images, which serve to continuously separate and purify cells that have different properties in that they are either attracted to or repelled by an induction electrophoresis force of a predetermined frequency; and a function for identifying cells on the basis of this shape and fluorescent emission information and thereby separating and purifying the cells.
Abstract translation: 提供了用于浓缩和分离细胞的装置，其具有用于连续浓缩细胞的功能; 然后连续地将浓缩的细胞排列在流路的预定区域中的功能; 基于细胞浓度和纯化图像同时鉴定单细胞单位形状和荧光发射的功能，其用于连续分离和纯化具有不同性质的细胞，因为它们被感应电泳力吸引或排斥 的预定频率; 以及基于该形状和荧光发射信息鉴别细胞，从而分离和纯化细胞的功能。
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Patent Agency Ranking
公开(公告)号：US09023294B2
公开(公告)日：2015-05-05
申请号：US13261410
申请日：2011-02-24
Applicant: Hideyuki Terazono , Kenji Yasuda , Masahito Hayashi , Hiroyuki Takei , Akihiro Hattori
Inventor： Hideyuki Terazono , Kenji Yasuda , Masahito Hayashi , Hiroyuki Takei , Akihiro Hattori
CPC classification number: G01N1/31 , C12N1/066 , G01N1/34 , G01N1/4077 , G01N15/1459 , G01N15/1463 , G01N21/6408 , G01N21/6428 , G01N2001/4094 , G01N2015/149 , G01N2021/6482 , Y10T436/11
Abstract: Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof.
Abstract translation: 提供一种细胞浓缩和净化装置，其具有：连续浓缩细胞的功能; 随后随后将细胞连续地放置在通道的特定区域中的功能; 基于图像同时识别每个单个细胞的形状和荧光发射的功能; 以及基于与其形状和荧光发射有关的数据识别细胞，然后分离和纯化细胞的功能。
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公开(公告)号：US08008069B2
公开(公告)日：2011-08-30
申请号：US10544799
申请日：2004-02-19
Applicant: Akihiro Hattori , Kenji Yasuda
Inventor： Akihiro Hattori , Kenji Yasuda
CPC classification number: C12M23/16 , B01J2219/00317 , B01J2219/00441 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/0063 , B01J2219/00637 , B01J2219/00743 , B01L3/5085 , B01L2200/12 , B01L2300/0819 , B01L2300/12 , B01L2300/163
Abstract: This invention relates to a method and apparatus for process cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source. The absorption layer has absorbency in visible and infrared regions, and the gel-like material is a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions. The light source is monochromatic light in the specific wave length. The light source is disposed such that it irradiates on the absorption layer and/or gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.
Abstract translation: 本发明涉及一种用于过程细胞培养的方法和装置。 该装置包括一个微室，该微室包括不超过一个吸收层和至少一个凝胶层，依次层叠在透明基板上，在可见光和红外区域中没有明显的吸收性，以及至少一个光源。 吸收层在可见光和红外区域具有吸收性，凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物，在室温下处于凝胶状态，具有吸收性 特定波长的可见光和红外区域。 光源是特定波长的单色光。 光源被设置成使其照射在吸收层和/或凝胶层上，除了当没有设置吸收层时，每个由凝胶状材料构成的至少两层层叠在透明基板上。
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公开(公告)号：US08247222B2
公开(公告)日：2012-08-21
申请号：US13036600
申请日：2011-02-28
Applicant: Akihiro Hattori , Kenji Yasuda
Inventor： Akihiro Hattori , Kenji Yasuda
CPC classification number: C12M23/16 , B01J2219/00317 , B01J2219/00441 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/0063 , B01J2219/00637 , B01J2219/00743 , B01L3/5085 , B01L2200/12 , B01L2300/0819 , B01L2300/12 , B01L2300/163
Abstract: This invention relates to a method and apparatus for processing cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, the absorption layer having absorbency in visible and infrared regions, and the gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and the light source having a monochromatic light in the specific wave length, wherein the light source is disposed such that it irradiates on the absorption layer and/or the gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.
Abstract translation: 本发明涉及一种用于处理细胞培养物的方法和装置。 该装置包括一个微室，该微室包括不超过一个吸收层和至少一个凝胶层，依次层叠在透明基板上，在可见光和红外区域中没有明显的吸收性，以及至少一个光源，吸收 在可见光和红外区域具有吸收性的层，凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物，在室温下处于凝胶状态，具有特定的吸光度 可见光和红外区域的波长，以及具有特定波长的单色光的光源，其中光源被设置成使其照射在吸收层和/或凝胶层上，除了当没有吸收 在透明基板上层叠由凝胶状材料构成的至少两层。
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公开(公告)号：US20080003678A1
公开(公告)日：2008-01-03
申请号：US11819681
申请日：2007-06-28
Applicant: Akihiro Hattori , Hideyuki Terazono , Kenji Yasuda
Inventor： Akihiro Hattori , Hideyuki Terazono , Kenji Yasuda
CPC classification number: B01L3/502761 , B01L3/502753 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0415 , C12M45/00 , C12M47/04 , G01N15/1031 , G01N15/12 , G01N15/1459 , G01N15/1463 , G01N15/1484 , G01N2015/1081 , G01N2015/149
Abstract: An inexpensive cell analysis and separation apparatus using a flow channel formed on one surface of a substrate and a chip replaceable for each sample, and a method for culturing the separated cells without contamination, are provided. A flow channel for allowing a cell-containing buffer solution to flow is provided. Cells are detected in the middle of the flow channel, and separated to a plurality of downstream flow channels based on whether each cell fulfills a predetermined condition. A culturing tank for collecting the condition-fulfilling cells is covered with a semipermeable membrane at a top surface so as to prevent contamination during cell separation. When the cell separation is finished, the flow channel communicated with the culturing tank accommodating the condition-fulfilling cells is closed, and the culturing tank is cut off from the apparatus and put into a culturing device containing a predetermined medium to culture the cells.
Abstract translation: 提供了使用形成在基板的一个表面上的流动通道和可替代每个样品的芯片的便宜的电池分析和分离装置，以及用于培养分离的电池而不被污染的方法。 提供了允许含细胞的缓冲溶液流动的流动通道。 基于每个小区是否满足预定条件，在流信道的中间检测小区，并将其分离成多个下游流信道。 用于收集状态满足细胞的培养槽在顶表面被半透膜覆盖，以防止细胞分离过程中的污染。 当细胞分离完成时，与容纳条件满足细胞的培养箱连通的流动通道关闭，并将培养箱从装置中切下并放入含有预定培养基的培养装置中以培养细胞。
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公开(公告)号：US20060141619A1
公开(公告)日：2006-06-29
申请号：US10544799
申请日：2004-02-19
Applicant: Akihiro Hattori , Kenji Yasuda
Inventor： Akihiro Hattori , Kenji Yasuda
CPC classification number: C12M23/16 , B01J2219/00317 , B01J2219/00441 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/0063 , B01J2219/00637 , B01J2219/00743 , B01L3/5085 , B01L2200/12 , B01L2300/0819 , B01L2300/12 , B01L2300/163
Abstract: There is provided a processing apparatus and a processing method for micro-chamber for cell culture having a desired culturing space. The micro-chamber processing apparatus for cell culture of the present invention comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, said absorption layer having absorbency in visible and infrared regions, and said gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and said light source having a monochromatic light in said specific wave length, wherein said light source is disposed such that it irradiates on said absorption layer and/or said gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on said transparent base plate.
Abstract translation: 提供了具有期望的培养空间的用于细胞培养的微室的处理装置和处理方法。 本发明的细胞培养用微细胞处理装置，具有不超过一个吸收层和至少一层凝胶层的微室，依次层叠在透明基板上，在可见光和红外线中不具有明显的吸收性 区域和至少一个光源，所述吸收层在可见光和红外区域具有吸收性，所述凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物 在具有特定波长的可见光和红外区域具有吸收能力，并且所述光源具有所述特定波长的单色光，其中所述光源被设置成使得其照射在所述吸收层和/或 所述凝胶层除了没有设置吸收层之外，将由凝胶状材料构成的至少两层层合在所述透明体b上 灰板
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