公开(公告)号：US20120283119A1

公开(公告)日：2012-11-08

申请号：US13493534

申请日：2012-06-11

Applicant: Yuji Miyahara ,  Kenji Yasuda ,  Kumiko Hattori

Inventor： Yuji Miyahara ,  Kenji Yasuda ,  Kumiko Hattori

IPC: C40B30/04

CPC classification number: B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/5085 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/06 ,  G01N27/4145 ,  C12Q2565/501 ,  C12Q2565/607

Abstract: A DNA microarray system whereby measurement can be performed at a low running cost, a low price and yet a high accuracy. A nucleic acid probe (3) is immobilized on the surface of a gate insulator of an electric field effect transistor and then hybridized with a target gene on the surface of the gate insulator. A change in the surface electric charge density thus arising is detected by using the electric effect.

Abstract translation: 一种DNA微阵列系统，其可以以低运行成本，低价格和高精度进行测量。 将核酸探针（3）固定在电场效应晶体管的栅极绝缘体的表面上，然后与栅极绝缘体的表面上的目标基因杂交。 通过使用电效应来检测由此产生的表面电荷密度的变化。

公开(公告)号：US20050170347A1

公开(公告)日：2005-08-04

申请号：US10499005

申请日：2001-12-19

Applicant: Yuji Miyahara ,  Kenji Yasuda ,  Kumiko Hattori

Inventor： Yuji Miyahara ,  Kenji Yasuda ,  Kumiko Hattori

IPC: B01J19/00 ,  B01L3/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  G01N27/414 ,  H01L29/06 ,  H01L29/66 ,  C12M1/34

CPC classification number: B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/5085 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/06 ,  G01N27/4145 ,  C12Q2565/501 ,  C12Q2565/607

Abstract: A DNA microarray system whereby measurement can be performed at a low running cost, a low price and yet a high accuracy. A nucleic acid probe (3) is immobilized on the surface of a gate insulator of an electric field effect transistor and then hybridized with a target gene on the surface of the gate insulator. A change in the surface electric charge density thus arising is detected by using the electric effect.

Abstract translation: 一种DNA微阵列系统，其可以以低运行成本，低价格和高精度进行测量。 将核酸探针（3）固定在电场效应晶体管的栅极绝缘体的表面上，然后与栅极绝缘体的表面上的目标基因杂交。 通过使用电效应来检测由此产生的表面电荷密度的变化。

公开(公告)号：US07273704B2

公开(公告)日：2007-09-25

申请号：US10522991

申请日：2002-08-12

Applicant: Takuya Matsui ,  Yuji Miyahara ,  Kumiko Hattori

Inventor： Takuya Matsui ,  Yuji Miyahara ,  Kumiko Hattori

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6825 ,  B01J2219/00286 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00662 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00722 ,  C12Q1/6837 ,  G01N27/4145 ,  G01N33/54373 ,  C12Q2565/607 ,  C12Q2565/501 ,  C12Q2545/114

Abstract: A nucleic acid contained in a sample is quantified with high accuracy. The procedure includes the step of allowing the sample containing a specific nucleic acid to interact with a DNA microarray provided with a plurality of nucleic acid probe parts having a nucleic acid probe capable of hybridizing to the specific nucleic acid; the step of monitoring outputs from each of the plurality of nucleic acid probe parts due to hybridization between the nucleic acid probe and the specific nucleic acid to determine normal distribution of time taken to reach a predetermined output value with respect to each of the plurality of nucleic acid probe parts; and the step of quantifying the specific nucleic acid contained in the sample based on a maximum value determined from the normal distribution obtained in the above step.

Abstract translation: 以高精度量化样品中所含的核酸。 该方法包括允许含有特定核酸的样品与提供有多个具有能够与特定核酸杂交的核酸探针的多个核酸探针部分的DNA微阵列相互作用的步骤; 由于核酸探针和特定核酸之间的杂交，监测来自多个核酸探针部分中的每一个的输出的步骤，以确定相对于多个核酸中的每一个核酸达到预定输出值所需的时间的正态分布 酸性探针部件; 以及基于从上述步骤中获得的正态分布确定的最大值来量化样品中所含特定核酸的步骤。

公开(公告)号：US20060088839A1

公开(公告)日：2006-04-27

申请号：US10522991

申请日：2002-08-12

Applicant: Takuya Matsui ,  Yuji Miyahara ,  Kumiko Hattori

Inventor： Takuya Matsui ,  Yuji Miyahara ,  Kumiko Hattori

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: C12Q1/6825 ,  B01J2219/00286 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00662 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00722 ,  C12Q1/6837 ,  G01N27/4145 ,  G01N33/54373 ,  C12Q2565/607 ,  C12Q2565/501 ,  C12Q2545/114

Abstract: A nucleic acid contained in a sample is quantified with high accuracy. The procedure includes the step of allowing the sample containing a specific nucleic acid to interact with a DNA microarray provided with a plurality of nucleic acid probe parts having a nucleic acid probe capable of hybridizing to the specific nucleic acid; the step of monitoring outputs from each of the plurality of nucleic acid probe parts due to hybridization between the nucleic acid probe and the specific nucleic acid to determine normal distribution of time taken to reach a predetermined output value with respect to each of the plurality of nucleic acid probe parts; and the step of quantifying the specific nucleic acid contained in the sample based on a maximum value determined from the normal distribution obtained in the above step.

Abstract translation: 以高精度量化样品中所含的核酸。 该方法包括允许含有特定核酸的样品与提供有多个具有能够与特定核酸杂交的核酸探针的多个核酸探针部分的DNA微阵列相互作用的步骤; 由于核酸探针和特定核酸之间的杂交，监测来自多个核酸探针部分中的每一个的输出的步骤，以确定相对于多个核酸中的每一个核酸达到预定输出值所需的时间的正态分布 酸性探针部件; 以及基于从上述步骤中获得的正态分布确定的最大值来量化样品中所含特定核酸的步骤。

公开(公告)号：US20120283403A1

公开(公告)日：2012-11-08

申请号：US13520710

申请日：2010-12-27

Applicant: Akira Matsumoto ,  Takehiko Ishii ,  Kazunori Kataoka ,  Yuji Miyahara

Inventor： Akira Matsumoto ,  Takehiko Ishii ,  Kazunori Kataoka ,  Yuji Miyahara

IPC: C07F5/02 ,  C08F230/06 ,  C08F30/06

CPC classification number: C07F5/025 ,  C08F220/56

Abstract: Disclosed are a phenylboronic acid monomer and a phenylboronic acid polymer, each of which can have a pKa value suitable for the use under physiological environments and can be used for various applications. The pheanylboronic acid monomer has a structure represented by formula (13) and therefore has high hydrophilicity, can have a satisfactorily low pKa value when the phenyl ring is fluorinated, and can have a polymerizable unsaturated bond. The phenylboronic acid monomer has high hydrophilicity at a pKa value of 7.4 or less which is a physiological level, and can be polymerized with a wide variety of monomers to produce polymers suitable for the intended purposes.

Abstract translation: 公开了苯基硼酸单体和苯基硼酸聚合物，其各自可以具有适合在生理环境下使用的pKa值，并可用于各种应用。 苯基苯基硼酸单体具有式（13）所示的结构，因此具有高亲水性，当苯基环被氟化时，可以具有令人满意的低pKa值，并且可以具有可聚合的不饱和键。 苯基硼酸单体在pKa值为7.4或更低的生物水平下具有高亲水性，并且可以与各种单体聚合以产生适合于预期目的的聚合物。

公开(公告)号：US07888013B2

公开(公告)日：2011-02-15

申请号：US11574353

申请日：2005-08-26

Applicant: Yuji Miyahara ,  Toshiya Sakata

Inventor： Yuji Miyahara ,  Toshiya Sakata

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: B01L7/00 ,  B01L3/5027 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  G01N27/4145

Abstract: Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary.A field-effect device for gene detection of the present invention analyzes a base sequence by immobilizing a single-strand nucleic acid probe at a gate portion, inducing hybridization at the gate portion to form a double-stranded DNA, inducing elongation reaction by adding a DNA polymerase and one of the substrates, and measuring the electrical characteristic of the field-effect device caused by elongation reaction.Since the elongation reaction of one base induced at the gate portion can be directly converted to an electrical signal, expensive lasers or complex optical systems are not needed. Thus, a small gene polymorphism detection system that can conduct measurement at high precision can be provided.

Abstract translation: 由于常规DNA序列分析技术是基于荧光检测的基本原理，因此必须使用昂贵的复杂光学系统和激光源。 本发明的用于基因检测的场效应装置通过在门部固定单链核酸探针来分析碱基序列，在门部诱导杂交以形成双链DNA，通过加入 DNA聚合酶和其中一种底物，并测量由伸长反应引起的场效应装置的电特性。 由于在栅极部分引起的一个基极的伸长反应可以直接转换成电信号，所以不需要昂贵的激光器或复杂的光学系统。 因此，可以提供可以高精度地进行测量的小型基因多态性检测系统。

公开(公告)号：US20100197001A1

公开(公告)日：2010-08-05

申请号：US12696342

申请日：2010-01-29

Applicant: Yuji Miyahara ,  Toshiya Sakata

Inventor： Yuji Miyahara ,  Toshiya Sakata

IPC: C12M1/00

CPC classification number: C12Q1/6825 ,  C12Q2600/156 ,  G01N27/4145 ,  C12Q2565/607 ,  C12Q2565/537

Abstract: A gene detection field-effect device provided with an insulation film (2), a semiconductor substrate (3), and a reference electrode (4), includes: (a) the insulation film (2) including a nucleic acid probe (5) immobilized on one of the surfaces thereof and is in contact with a sample solution (6) containing at least one type of a target gene (601) for detection and analysis; (b) the semiconductor substrate (3) being installed so as to abut against the other surface of the insulation film (2); and (c) the reference electrode (4) being provided in the sample solution (6).

Abstract translation: 设置有绝缘膜（2），半导体基板（3）和参考电极（4）的基因检测场效应元件包括：（a）包含核酸探针（5）的绝缘膜（2） 固定在其一个表面上并与含有至少一种类型的目标基因（601）的样品溶液（6）接触以进行检测和分析; （b）半导体衬底（3）被安装成抵靠绝缘膜（2）的另一个表面; 和（c）参考电极（4）设置在样品溶液（6）中。

公开(公告)号：US07695907B2

公开(公告)日：2010-04-13

申请号：US10587941

申请日：2005-02-03

Applicant: Yuji Miyahara ,  Toshiya Sakata

Inventor： Yuji Miyahara ,  Toshiya Sakata

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  C12Q2600/156 ,  G01N27/4145 ,  C12Q2565/607 ,  C12Q2565/537

Abstract: A gene detection field-effect device provided with an insulation film (2), a semiconductor substrate (3), and a reference electrode (4), includes: (a) the insulation film (2) including a nucleic acid probe (5) immobilized on one of the surfaces thereof and is in contact with a sample solution (6) containing at least one type of a target gene (601) for detection and analysis; (b) the semiconductor substrate (3) being installed so as to abut against the other surface of the insulation film (2); and (c) the reference electrode (4) being provided in the sample solution (6).

Abstract translation: 设置有绝缘膜（2），半导体基板（3）和参考电极（4）的基因检测场效应元件包括：（a）包含核酸探针（5）的绝缘膜（2） 固定在其一个表面上并与含有至少一种类型的目标基因（601）的样品溶液（6）接触以进行检测和分析; （b）半导体衬底（3）被安装成抵靠绝缘膜（2）的另一个表面; 和（c）参考电极（4）设置在样品溶液（6）中。

公开(公告)号：US06381015B1

公开(公告)日：2002-04-30

申请号：US09380268

申请日：1999-08-27

Applicant: Tsuyoshi Sonehara ,  Yuji Miyahara ,  Masao Suga

Inventor： Tsuyoshi Sonehara ,  Yuji Miyahara ,  Masao Suga

IPC: G01B902

CPC classification number: G01N21/45 ,  A61B5/14532 ,  A61B5/1455 ,  A61B5/7257 ,  G01J2003/4538

Abstract: According to an aspect of the present invention, there is provided an inspection apparatus using an optical interferometer including splitting and combining means (3) for splitting light from a light source (1) into incident light (41) irradiated on a sample and reference light (40) and combining signal light which is light scattered or reflected by the sample (7, 42) and the reference light, a modulator (4, 5, 10) for subjecting the reference light to phase modulation and a photo detector (9) for detecting light combined by the splitting and combining means (3), the inspection apparatus further including first detecting means (12-1) for detecting amplitudes of first signal components having frequencies of multiples of odd numbers of a fundamental modulation frequency of the modulator in a signal from the photo detector (9), second detecting means (12-2) for separating and detecting amplitudes of second signal components having frequencies of multiples of even numbers of the fundamental modulation frequency in the signal from the photo detector and means (14) for calculating an intensity of the signal light by using the amplitudes of the first and the second signal components for providing high signal stability and signal-to-noise ratio and in measuring a sample of a biomedical tissue or the like having large scattering or attenuation of light, distributions of a refractive index and an extinction coefficient up to a portion having a large depth can be measured with high accuracy.

Abstract translation: 根据本发明的一个方面，提供了一种使用光学干涉仪的检查装置，该光学干涉仪包括用于将来自光源（1）的光分解成照射在样品上的入射光（41）和参考光 （40），并且组合由所述样品（7,42）和所述参考光散射或反射的信号光，用于使所述参考光进行相位调制的调制器（4,5,10）和光电检测器（9） 用于检测由分割和组合装置（3）组合的光，检查装置还包括第一检测装置（12-1），用于检测具有调制器的基本调制频率的奇数倍数的第一信号分量的振幅 来自光检测器（9）的信号，第二检测装置（12-2），用于分离和检测具有基数的偶数倍数的第二信号分量的振幅 来自光电检测器的信号中的调制频率和用于通过使用用于提供高信号稳定性和信噪比的第一和第二信号分量的幅度来计算信号光强度的装置（14） 可以高精度地测量具有大的散射或衰减光的生物医学组织样品，折射率和消光系数的分布直到具有较大深度的部分。

公开(公告)号：US06346383B1

公开(公告)日：2002-02-12

申请号：US09527234

申请日：2000-03-16

Applicant: Tomoharu Kajiyama ,  Yuji Miyahara ,  Katsuji Murakawa

Inventor： Tomoharu Kajiyama ,  Yuji Miyahara ,  Katsuji Murakawa

IPC: C12Q168

CPC classification number: B01L7/52 ,  B01J19/0046 ,  B01J2219/00495 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01L3/50851 ,  B01L7/54 ,  B01L2300/0636 ,  B01L2300/0819 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  Y10S977/924 ,  C12Q2527/107

Abstract: A biochemical reaction detection chip capable of controlling the temperature for biochemical reactions including hybridizations and its substrate. The function of the chip is performed by comprising a plurality of islands of a heat conducting material on the membrane of the substrate, the islands being spaced from each other and individually provided with temperature controllers, and the probes immobilized on the substrate.

Abstract translation: 一种生化反应检测芯片，能够控制生化反应的温度，包括杂交及其底物。 芯片的功能是通过在衬底的膜上包括多个导热材料的岛来实现的，这些岛彼此间隔开并且分别设置有温度控制器，并且探针固定在衬底上。