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    Method and apparatus for sample preparation
    1.
    发明申请
    Method and apparatus for sample preparation 审中-公开
    样品制备方法和装置

    公开(公告)号：US20080241841A1

    公开(公告)日：2008-10-02

    申请号：US11984065

    申请日：2007-11-13

    申请人： Katsuji Murakawa ,  Sumiyo Takiguchi ,  Hideki Kambara

    发明人： Katsuji Murakawa ,  Sumiyo Takiguchi ,  Hideki Kambara

    IPC分类号： C12Q1/68 ,  C12P21/04 ,  C12M1/34

    CPC分类号： C12Q1/6806 ,  C12Q1/6851 ,  C12Q2527/143 ,  C12Q2563/173

    摘要： A method of the present invention comprises fractionating a sample solution containing analyte DNA molecules into small droplets, wherein the number M of the droplets is greater than the total number N of the DNA molecules, subjecting an emulsion containing the droplets to, for example, PCR amplification, and detecting the presence or absence (amount) of an amplicon obtained in each droplet by fluorescent detection using an intercalator or the like.

    摘要翻译： 本发明的方法包括将含有分析物DNA分子的样品溶液分级成小液滴，其中液滴的数量M大于DNA分子的总数N，使含有液滴的乳液经受例如PCR 通过使用嵌入剂等进行荧光检测，检测各液滴中获得的扩增子的存在或不存在（量）。

    Centrifugal separator and sample preparation device using the separator
    2.
    发明授权
    Centrifugal separator and sample preparation device using the separator 失效
    离心分离器和使用分离器的样品制备装置

    公开(公告)号：US06808633B1

    公开(公告)日：2004-10-26

    申请号：US10018430

    申请日：2001-12-20

    申请人： Kazunori Okano ,  Katsuji Murakawa ,  Shinichi Fukuzono ,  Yukiko Ikeda

    发明人： Kazunori Okano ,  Katsuji Murakawa ,  Shinichi Fukuzono ,  Yukiko Ikeda

    IPC分类号： B01D1100

    CPC分类号： B01L3/5021 ,  B04B1/02 ,  B04B5/0407 ,  B04B5/0442 ,  B04B5/10 ,  B04B7/08 ,  Y10T436/111666 ,  Y10T436/25375 ,  Y10T436/255

    摘要： A centrifugal separator of the invention has a centrifugal rotor (10-1), with symmetric rotation axes, having single sample separation chamber in it for centrifuging samples contained in sample solutions, an upper opening passing through to said sample separation chamber in the upper part, members of frameworks capable of being coupled to said upper opening, a rotation driving means, assuming that the direction of said symmetric rotation axis is the first direction, for driving said centrifugal rotor around said rotation axis in the first direction, wherein assuming that two directions intersecting with said first direction are the second and third directions, the length of said sample preparation chamber in said third direction is longer than the length of said sample preparation chamber in said second direction. Since one kind of sample is handled in one centrifugal rotor, the centrifugal separator of the invention allows discrete treatment (sequential treatment) suitable for the flow system, in which individual samples can be treated independently, making easy automation of sample preparation revolving centrifugation.

    摘要翻译： 本发明的离心分离机具有对称旋转轴的离心转子（10-1），在其中具有单个样品分离室，用于离心样品溶液中包含的样品，上部开口穿过上部样品分离室 假定所述对称旋转轴的方向为第一方向的旋转驱动装置，用于沿所述第一方向围绕所述旋转轴驱动所述离心转子，其中，假定所述两个 与第一方向相交的方向是第二和第三方向，所述样品制备室在所述第三方向上的长度比所述第二方向上的样品制备室的长度长。 由于在一个离心转子中处理了一种样品，本发明的离心分离器允许适用于流动系统的独立处理（顺序处理），其中可以独立地处理各个样品，从而容易地自动进行样品制备旋转离心。

    Automobile transmission casing having reinforcement
    6.
    发明授权
    Automobile transmission casing having reinforcement 失效
    汽车变速箱外壳加强

    公开(公告)号：US4435994A

    公开(公告)日：1984-03-13

    申请号：US277603

    申请日：1981-06-26

    申请人： Shuji Hata ,  Toshio Yamada ,  Katsuji Murakawa

    发明人： Shuji Hata ,  Toshio Yamada ,  Katsuji Murakawa

    IPC分类号： F16H3/091 ,  F16H57/02 ,  F16H57/028 ,  F16H57/03

    CPC分类号： F16H57/028 ,  F16H57/021 ,  F16H57/03 ,  F16H2057/02043 ,  F16H3/0915 ,  Y10T74/2186

    摘要： An automobile transmission including a peripheral wall having an inner surface comprised in a cross-section of a plurality of curved portions which are continuous in series with each other through inwardly projecting node portions. The configuration of the casing provides a structure of high rigidity so that it is effective to suppress the vibrations of the casing wall and consequently the noise caused by such vibrations. The casing wall is formed with brackets for supporting the reverse idle gear shaft in the vicinity of one of the node portions. This arrangement is also effective to suppress vibrations.

    摘要翻译： 一种汽车变速器，其包括具有内表面的周壁，该内表面包括在通过向内突出的节点部分彼此串联连续的多个弯曲部分的横截面中。 壳体的构造提供了高刚性的结构，使得有效地抑制壳体壁的振动，并因此有效地抑制由这种振动引起的噪声。 壳体壁形成有用于在其中一个节点部分附近支撑反向空转齿轮轴的支架。 这种布置也有效地抑制振动。