Invention Grant
US07888013B2 Method of analyzing DNA sequence using field-effect device, and base sequence analyzer
有权
使用场效应分析DNA序列的方法和碱基序列分析仪
- Patent Title: Method of analyzing DNA sequence using field-effect device, and base sequence analyzer
- Patent Title (中): 使用场效应分析DNA序列的方法和碱基序列分析仪
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Application No.: US11574353Application Date: 2005-08-26
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Publication No.: US07888013B2Publication Date: 2011-02-15
- Inventor: Yuji Miyahara , Toshiya Sakata
- Applicant: Yuji Miyahara , Toshiya Sakata
- Applicant Address: JP Tsukuba-shi
- Assignee: National Institute for Materials Science
- Current Assignee: National Institute for Materials Science
- Current Assignee Address: JP Tsukuba-shi
- Agency: Westerman, Hattori, Daniels & Adrian, LLP
- Priority: JP2004-248258 20040827
- International Application: PCT/JP2005/015522 WO 20050826
- International Announcement: WO2006/022370 WO 20060302
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/02 ; C07H21/04

Abstract:
Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary.A field-effect device for gene detection of the present invention analyzes a base sequence by immobilizing a single-strand nucleic acid probe at a gate portion, inducing hybridization at the gate portion to form a double-stranded DNA, inducing elongation reaction by adding a DNA polymerase and one of the substrates, and measuring the electrical characteristic of the field-effect device caused by elongation reaction.Since the elongation reaction of one base induced at the gate portion can be directly converted to an electrical signal, expensive lasers or complex optical systems are not needed. Thus, a small gene polymorphism detection system that can conduct measurement at high precision can be provided.
Public/Granted literature
- US20080286767A1 Method of Analyzing Dna Sequence Using Field-Effect Device, and Base Sequence Analyzer Public/Granted day:2008-11-20
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