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    MUTANT GLUCOSE DEHYDROGENASE
    14.
    发明申请
    MUTANT GLUCOSE DEHYDROGENASE 有权

    公开(公告)号：US20100086989A1

    公开(公告)日：2010-04-08

    申请号：US12556142

    申请日：2009-09-09

    申请人： Hideaki Yamaoka ,  Masashi Tsukada

    发明人： Hideaki Yamaoka ,  Masashi Tsukada

    IPC分类号： C07H21/04 ,  C12N1/21

    CPC分类号： C12Q1/32 ,  C12N9/0006 ,  C12Q1/54 ,  C12Y101/01049

    摘要： A mutant glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 3 or an amino acid sequence of SEQ ID NO: 3 including substitution, deletion, insertion or addition of one or more amino acid residues other than the amino acid residue at the 365th position and having glucose dehydrogenase activity, wherein an amino acid residue at a position corresponding to the 365th position of, the amino acid sequence is replaced with another amino acid residue, and the mutant glucose dehydrogenase shows an improved substrate specificity to glucose.

    摘要翻译： 具有SEQ ID NO：3的氨基酸序列或SEQ ID NO：3的氨基酸序列的突变体葡萄糖脱氢酶，包括在第365天除了氨基酸残基之外的一个或多个氨基酸残基的取代，缺失，插入或添加 位置并且具有葡萄糖脱氢酶活性，其中与氨基酸序列的第365位相对应的位置的氨基酸残基被另一氨基酸残基替代，并且突变型葡萄糖脱氢酶显示出对葡萄糖的底物特异性改善。

    Novel glucose-6-phosphate dehydrogenase
    15.
    发明申请
    Novel glucose-6-phosphate dehydrogenase 有权
    新型葡萄糖-6-磷酸脱氢酶

    公开(公告)号：US20040171130A1

    公开(公告)日：2004-09-02

    申请号：US10312007

    申请日：2002-12-23

    发明人： Haruhiko Yokoi ,  Seiko Ando ,  Keiko Ochiai ,  Yoshiyuki Yonetani ,  Shin-ichi Hashimoto

    IPC分类号： C12N009/04 ,  C07H021/04

    CPC分类号： C12N9/0006 ,  C12P13/04 ,  C12P13/08 ,  C12Y101/01049

    摘要： The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as nullG6PDnull) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant. According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.

    摘要翻译： 本发明涉及一种衍生自属于棒状杆菌属的细菌的新型葡萄糖-6-磷酸脱氢酶（以下称为“G6PD”），编码该酶的DNA，包含该DNA的重组DNA，包含重组体 DNA，包含其染色体上的DNA的转化体，以及生产L-氨基酸或G6PD的方法，其包括培养转化体。 根据本发明，获得了修饰的G6PD和编码G6PD的D​​NA，通过使用改良的G6PD可以提高微生物的L-氨基酸的生产率。

    Hybrid enzyme and use thereof
    16.
    发明申请
    Hybrid enzyme and use thereof 审中-公开
    杂交酶及其用途

    公开(公告)号：US20020081690A1

    公开(公告)日：2002-06-27

    申请号：US09879257

    申请日：2001-06-12

    发明人： Sachiko Yamamoto ,  Minoru Shiro ,  Toshiro Hanada ,  Shinzo Kobatake

    IPC分类号： G01N033/53 ,  G01N033/537 ,  G01N033/543 ,  C12Q001/44 ,  C12Q001/32 ,  C12N009/04 ,  C12N009/16

    CPC分类号： C12N9/0006 ,  C07K14/4737 ,  C07K2319/00 ,  C12N9/16 ,  C12N9/2471 ,  C12Y101/01049 ,  C12Y302/01023

    摘要： Disclosed is a hybrid enzyme containing a foreign peptide, the hybrid enzyme having an enzyme activity similar to that prior to the substitution or insertion of the peptide, and having a property that the hybrid enzyme activity is modulated or modified when a material having binding ability to the peptide introduced by substitution or insertion is bound to the peptide moiety. Using the hybrid enzyme, it becomes possible to assay a trace amount of CRP in a sample by a homogeneous colorimetry, and a macromolecule materials can be easily assayed in a homogeneous system.

    摘要翻译： 公开了含有外来肽的杂合酶，所述杂交酶具有与肽取代或插入前相似的酶活性，并且具有当具有结合能力的材料具有调节或修饰的性质时 通过取代或插入引入的肽与肽部分结合。 使用混合酶，可以通过均匀比色法测定样品中痕量的CRP，并且可以容易地在均相系统中测定大分子材料。