公开(公告)号：US20030207431A1

公开(公告)日：2003-11-06

申请号：US10341692

申请日：2003-01-14

发明人： Mitali Ghoshal ,  Gerald Sigler ,  Raymond A. Hui ,  Ronald Hawley ,  Eva Hoess ,  Erasmus J. Huber ,  John W. Patterson ,  Salvatore J. Salamone ,  Allan Dorn

IPC分类号： C12Q001/32 ,  C12N009/00 ,  A61K039/00

CPC分类号： C07D405/12 ,  C07D307/88 ,  C07D473/08 ,  C07H15/20 ,  C07H15/22 ,  C07H15/24 ,  C07J17/00 ,  C07J19/005 ,  C07J41/0005 ,  C07J41/0055 ,  C07J43/003 ,  G01N33/53 ,  G01N33/535 ,  G01N33/5735 ,  G01N33/581 ,  G01N33/78 ,  G01N33/9453 ,  G01N2333/904

摘要： The present invention provides a method for the measurement of an analyte in biological samples whereby an uncompetitive inhibitor is coupled to a ligand and utilized in a homogeneous assay. The analyte can be a drug or drug derivative, hormone, polypeptide, or oligonucleotide. The present invention also provides novel compounds, assay reagents and packaged kits useful for performing such measurements.

摘要翻译： 本发明提供了一种用于测量生物样品中分析物的方法，由此使非竞争性抑制剂与配体偶联并用于均匀测定。 分析物可以是药物或药物衍生物，激素，多肽或寡核苷酸。 本发明还提供了可用于进行这种测量的新型化合物，测定试剂和包装的试剂盒。

公开(公告)号：US20020132283A1

公开(公告)日：2002-09-19

申请号：US09928434

申请日：2001-08-14

发明人： Makoto Inada ,  Nobuhiro Ikei ,  Hideji Nonomura ,  Yasuo Irie

IPC分类号： C12Q001/32

CPC分类号： A61K49/0004 ,  A61K49/1815 ,  Y10T436/24

摘要： The present invention provide a method for assessing the sensitivity to a pyrimidine drug such as 5-FU which is degraded in the pyrimidine metabolic pathway, specifically in vivo pyrimidine metabolizing activity, of an individual subject; and a preparation useful for the assessment. The present invention can be carried out by: administering a preparation comprising as an active ingredient a pyrimidine compound or its metabolite which acts as a substrate for a pyrimidine metabolizing enzyme, in which compound or metabolite at least one of C, O and N is labeled with an isotope; and assessing in vivo pyrimidine metabolizing activity based on the amount of excreted metabolite.

摘要翻译： 本发明提供了评估对于个体受试者的嘧啶代谢途径（特别是体内嘧啶代谢活性）降解的嘧啶药物如5-FU的敏感性的方法。 以及对评估有用的准备。 本发明可以通过以下方式进行：给予包含作为活性成分的嘧啶化合物或其代谢物的制剂，所述嘧啶化合物或其代谢物用作嘧啶代谢酶的底物，其中C，O和N中至少一个的化合物或代谢物被标记 与同位素; 并基于排泄代谢物的量评估体内嘧啶代谢活性。

公开(公告)号：US20010044128A1

公开(公告)日：2001-11-22

申请号：US09760205

申请日：2001-01-12

发明人： Ralf Zielenski

IPC分类号： C12Q001/32

CPC分类号： C12Q1/32

摘要： Stabilized aqueous solution of a coenzyme for hydrogen-transferring enzymes characterized in that the solution contains NAD, NADP or a derivative thereof in an oxidized or reduced form and one or several organic compounds or salts thereof having a pKa value between 1.5 and 6.0 and/or a nitrogen compound of the general formula (I) 1 in which the residues R1, R2 and R3 are the same or different and denote hydrogen, or a saturated or unsaturated alkyl or aryl group as well as the use of the solution to determine dehydrogenases in particular lactate dehydrogenase or substrates thereof.

摘要翻译： 稳定的用于氢转移酶的辅酶水溶液，其特征在于该溶液含有氧化或还原形式的NAD，NADP或其衍生物和一种或几种pKa值介于1.5和6.0之间的有机化合物或其盐和/或 其中残基R 1，R 2和R 3相同或不同并表示氢，或饱和或不饱和的烷基或芳基的通式（I）的氮化合物以及该溶液特别用于确定脱氢酶的用途 乳酸脱氢酶或其底物。

公开(公告)号：US20030203420A1

公开(公告)日：2003-10-30

申请号：US10124029

申请日：2002-04-17

发明人： Terry L. Riss ,  Richard A. Moravec

IPC分类号： C12Q001/32 ,  C12Q001/26

CPC分类号： G01N33/5014 ,  Y10S435/975

摘要： Disclosed are a method and a corresponding kit for determining the cytotoxicity of a test agent. The method includes the steps of adding a pre-determined amount of the test agent to a vessel containing living cells in culture medium. The cells are then incubated for a pre-determined amount of time. To the cells and culture medium is then added a reagent mixture that is non-toxic to the living cells. In the preferred embodiment, the reagent mixture contains a solvent, a dye, an electron transfer agent, a substrate for a cytoplasmic enzyme having a half-life greater than two hours and NADnull. The contents of the vessel are then measured for production of the reduced state of the dye, the production of the reduced state of the dye being caused by a cytoplasmic enzyme specific for the substrate in the reagent mixture. In the preferred embodiment, the production of the reduced state of the dye is caused by lactate dehydrogenase released from dead and dying cells within the vessel. The kits contain components necessary for performing the method.

摘要翻译： 公开了用于测定试剂的细胞毒性的方法和相应试剂盒。 该方法包括以下步骤：将预定量的测试剂加入到培养基中含有活细胞的容器中。 然后将细胞孵育预定量的时间。 然后向细胞和培养基中加入对活细胞无毒的试剂混合物。 在优选实施方案中，试剂混合物含有溶剂，染料，电子转移剂，半衰期大于2小时的细胞质酶底物和NAD +。 然后测量容器的内容物以产生染料的还原状态，染料的还原状态的产生由对试剂混合物中底物特异的细胞质酶引起。 在优选的实施方案中，染料的还原状态的产生是由容器内死亡和死亡细胞释放的乳酸脱氢酶引起的。 套件包含执行该方法所需的组件。

公开(公告)号：US20030170772A1

公开(公告)日：2003-09-11

申请号：US10388145

申请日：2003-03-12

发明人： Tianmei Ouyang ,  Paing Huang ,  Xiaoling Zheng

IPC分类号： C12Q001/26 ,  C12Q001/32 ,  A61K033/22

CPC分类号： C12Q1/26 ,  G01N33/52

摘要： Stabilized tetrazolium dye-phenazine reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include: (1) a tetrazolium dye component, e.g., a water soluble tetrazolium salt; (2) a phenazine component; and (3) an effective amount of one or more tetrazolium dye-phenazine stabilizing reagents, e.g., an inorganic Group IIIA compound and/or a flavin. In many embodiments, the subject reagent compositions include additional members of an analyte oxidizing signal producing system, such as: an analyte oxidizing enzyme, e.g., an analyte dehydrogenase or an analyte oxidase; and an enzyme cofactor. Also provided are test strips that include the subject reagent compositions, as well as systems and kits incorporating the subject test strips. The subject reagent compositions, test strips, systems and kits find use in the detection of a wide variety of analytes in a sample, such as a physiological sample, e.g., blood or a fraction thereof, or ISF (interstitial fluid).

摘要翻译： 提供稳定的四唑染料 - 吩嗪试剂组合物及其用于测量样品中分析物的方法。 主题试剂组合物包括：（1）四唑鎓染料组分，例如水溶性四唑鎓盐; （2）吩嗪组分; 和（3）有效量的一种或多种四唑染料 - 吩嗪稳定试剂，例如无机IIIA族化合物和/或黄素。 在许多实施方案中，主题试剂组合物包括分析物氧化信号产生系统的附加成员，例如：分析物氧化酶，例如分析物脱氢酶或分析物氧化酶; 和酶辅因子。 还提供了包括主题试剂组合物的测试条，以及包含主题测试条的系统和试剂盒。 主题试剂组合物，测试条，系统和试剂盒可用于检测样品中各种分析物，例如生理样品，例如血液或其部分，或ISF（间质液）。

公开(公告)号：US20020081690A1

公开(公告)日：2002-06-27

申请号：US09879257

申请日：2001-06-12

发明人： Sachiko Yamamoto ,  Minoru Shiro ,  Toshiro Hanada ,  Shinzo Kobatake

IPC分类号： G01N033/53 ,  G01N033/537 ,  G01N033/543 ,  C12Q001/44 ,  C12Q001/32 ,  C12N009/04 ,  C12N009/16

CPC分类号： C12N9/0006 ,  C07K14/4737 ,  C07K2319/00 ,  C12N9/16 ,  C12N9/2471 ,  C12Y101/01049 ,  C12Y302/01023

摘要： Disclosed is a hybrid enzyme containing a foreign peptide, the hybrid enzyme having an enzyme activity similar to that prior to the substitution or insertion of the peptide, and having a property that the hybrid enzyme activity is modulated or modified when a material having binding ability to the peptide introduced by substitution or insertion is bound to the peptide moiety. Using the hybrid enzyme, it becomes possible to assay a trace amount of CRP in a sample by a homogeneous colorimetry, and a macromolecule materials can be easily assayed in a homogeneous system.

摘要翻译： 公开了含有外来肽的杂合酶，所述杂交酶具有与肽取代或插入前相似的酶活性，并且具有当具有结合能力的材料具有调节或修饰的性质时 通过取代或插入引入的肽与肽部分结合。 使用混合酶，可以通过均匀比色法测定样品中痕量的CRP，并且可以容易地在均相系统中测定大分子材料。

公开(公告)号：US20030032078A1

公开(公告)日：2003-02-13

申请号：US09885303

申请日：2001-06-19

申请人： Board of Regents, The University of Texas System

发明人： Gabriel H. Travis

IPC分类号： C12Q001/32 ,  C12N009/04

CPC分类号： C12N9/0006 ,  A61K45/06 ,  C12Y101/01105 ,  G01N33/6893 ,  G01N2333/904

摘要： The present invention is a method for screening and identifying therapeutic agents for the treatment of macular or retinal degeneration. The candidate substances preferably reduces the activity of 11-cis-retinol dehydrogenase. In vitro and in vivo studies administering the inhibitor molecules to abcr knockout mice and analyzing for the inhibition of lipofuscin (A2E) accumulation are contemplated.

摘要翻译： 本发明是用于筛选和鉴定用于治疗黄斑或视网膜变性的治疗剂的方法。 候选物质优选降低11-顺式 - 视黄醇脱氢酶的活性。 预期给予抑制剂分子以抑制敲除小鼠并分析脂褐素（A2E）积聚的抑制的体外和体内研究。

公开(公告)号：US20020150958A1

公开(公告)日：2002-10-17

申请号：US10029654

申请日：2001-12-21

发明人： Birgit Jung ,  Stefan Mueller ,  Norbert Kraut

IPC分类号： C12Q001/48 ,  C12Q001/32

CPC分类号： C12Q1/48 ,  C12Q1/26 ,  G01N33/5055

摘要： The present invention relates to proteins involved in inflammatory processes and the modulation of the function of such a protein in order to positively influence inflammatory diseases.

摘要翻译： 本发明涉及涉及炎症过程的蛋白质和调节这种蛋白质的功能以积极地影响炎性疾病。

公开(公告)号：US20020068311A1

公开(公告)日：2002-06-06

申请号：US09509610

申请日：2000-03-30

发明人： HIDEAKI TANAKA ,  YOSHIKAZU AMANO

IPC分类号： C12Q001/32

CPC分类号： C12Q1/527 ,  C12Q1/32

摘要： This invention provides a method for the determination of bicarbonate ion existing in liquid using phosphenolpyruvate carboxylase and malate dehydrogenase as conjugate enzyme wherein thioNAD(P)H is used as substrate of malate dehydrogenase. The use of the thioNAD(P)H enables the measurement with visible light, and enables the simple and accurate determination of bicarbonate ion concentration of biological liquid sample in the clinical test.

摘要翻译： 本发明提供了使用磷酸烯醇丙酮酸羧化酶和苹果酸脱氢酶作为其中使用thioNAD（P）H作为苹果酸脱氢酶底物的共轭酶的液体中存在的碳酸氢根离子的测定方法。 使用thioNAD（P）H可以用可见光进行测量，并能够在临床试验中简单而准确地测定生物液体样品的碳酸氢根离子浓度。

公开(公告)号：US20030203421A1

公开(公告)日：2003-10-30

申请号：US10431668

申请日：2003-05-08

发明人： Tianmei Ouyang ,  Paing Huang

IPC分类号： C12Q001/32

CPC分类号： C12Q1/26 ,  G01N33/52

摘要： Stabilized tetrazolium dye reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include a tetrazolium dye component, e.g., a water soluble tetrazolium salt, and an effective amount of a nitrite stabilizing agent, e.g., a nitrite salt. In many embodiments, the subject reagent compositions include additional members of an analyte oxidizing signal producing system, such as: an analyte oxidizing enzyme, e.g., an analyte dehydrogenase or an analyte oxidase; an electron transfer agent; and an enzyme cofactor. Also provided are test strips that include the subject reagent compositions, as well as systems and kits incorporating the subject test strips. The subject reagent compositions, test strips, systems and kits find use in the detection of a wide variety of analytes in a sample, such as a physiological sample, e.g., blood or a fraction thereof, or ISF (interstitial fluid).