公开(公告)号：US20180245061A1

公开(公告)日：2018-08-30

申请号：US15524219

申请日：2015-11-04

申请人： THE UNIVERSITY COURT OF THE UNIVERSITY OF ABERDEEN ,  THE UNIVERSITY COURT OF THE UNIVERSITY OF ST. ANDREWS

发明人： James NAISMITH ,  Jesko KOEHNKE ,  Andrew BENT ,  Nicholas WESTWOOD ,  Greg Mann ,  Wael HOUSSEN IBRAHIM ,  Marcel JASPARS ,  Ying GE

IPC分类号： C12N9/90 ,  C12P21/02 ,  C07K1/107 ,  C12N9/00

CPC分类号： C12N9/90 ,  C07K1/107 ,  C12N9/00 ,  C12P21/02 ,  C12Y504/99027

摘要： This invention relates to an engineered leader-independent heterocyclase (also known as a cyclodehydratase) comprising a defined cyanobactin leader sequence which drives the efficient conversion of heterocyclisable amino acids, such as Ser, Thr and Cys, within a peptide substrate lacking a leader sequence into heterocycles produce a homogenous heterocycle-containing product. This may be useful in biotechnology and chemical synthesis.

公开(公告)号：US10059973B2

公开(公告)日：2018-08-28

申请号：US14414957

申请日：2013-07-18

申请人： Vland Biotech Group Co., Ltd.

发明人： Tom Tao Huang

IPC分类号： C12N1/14 ,  C12P21/00 ,  C07H13/04 ,  C12P19/12 ,  C12P19/44 ,  C12P21/02 ,  C12N1/38 ,  C12N9/26 ,  C12N9/42 ,  C12N15/80 ,  C07K14/475 ,  C07K14/575 ,  C07K16/00

CPC分类号： C12P21/005 ,  C07H13/04 ,  C07K14/475 ,  C07K14/575 ,  C07K16/00 ,  C12N1/14 ,  C12N1/38 ,  C12N9/2408 ,  C12N9/2411 ,  C12N9/2434 ,  C12N9/2437 ,  C12N15/80 ,  C12P19/12 ,  C12P19/44 ,  C12P21/02

摘要： The invention provides novel carbohydrate esters, in particular disaccharide esters, and the methods of their preparation. These compounds find use as microbial media components for the induction of gene expression in microbial fermentation processes.

公开(公告)号：US20180237847A1

公开(公告)日：2018-08-23

申请号：US15752884

申请日：2016-08-19

申请人： GENOMATICA, INC.

发明人： Stephanie CULLER ,  Ihsiung Brandon CHEN ,  Priti PHARKYA ,  Steve VAN DIEN ,  Nelson BARTON

IPC分类号： C12Q1/6869 ,  C12N15/09 ,  C12N5/16 ,  A61K36/02 ,  A61K35/66 ,  A61K36/06 ,  C40B40/04

CPC分类号： C12Q1/6869 ,  A61K35/66 ,  A61K36/02 ,  A61K36/06 ,  A61K2236/15 ,  A61K2236/30 ,  C12N5/16 ,  C12N15/09 ,  C12N2330/50 ,  C12P19/34 ,  C12P21/02 ,  C40B40/04 ,  Y02A50/409 ,  Y02A50/469 ,  A61K35/12

摘要： In alternative embodiments, provided herein are transcription/translation (TX-TL) systems and methods of using them for use as rapid prototyping platforms for the synthesis, modification and identification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites, from biosynthetic gene cluster pipelines. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the combinatorial biosynthesis of natural products (NPs), natural product analogs (NPAs) and secondary metabolites. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the rapid prototyping of complex biosynthetic pathways as a way to rapidly assess combinatorial and biosynthetic designs before moving to cellular hosts. In alternative embodiments, these exemplary TX-TL systems are multiplexed for high-throughput (HT) automation and for prototyping engineered platforms for the synthesis or modification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites analogs.

公开(公告)号：US10053496B2

公开(公告)日：2018-08-21

申请号：US15660572

申请日：2017-07-26

申请人： MOREHOUSE SCHOOL OF MEDICINE

发明人： Vincent Craig Bond ,  Michael Powell ,  Ming Bo Huang ,  Syed Ali

IPC分类号： C07K14/05 ,  C07K14/005 ,  C07K14/16 ,  G01N33/543 ,  A61K9/127 ,  A61K38/16 ,  A61K39/39 ,  A61K45/06 ,  G01N33/566 ,  C12P21/02 ,  C12N15/00 ,  A61K39/00 ,  A61K38/00

CPC分类号： C07K14/005 ,  A61K9/127 ,  A61K38/00 ,  A61K38/162 ,  A61K39/39 ,  A61K45/06 ,  A61K2039/55555 ,  C07K14/163 ,  C07K2319/00 ,  C07K2319/055 ,  C07K2319/50 ,  C07K2319/60 ,  C12N15/00 ,  C12N2740/16322 ,  C12P21/02 ,  G01N33/543 ,  G01N33/566

摘要： The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

公开(公告)号：US20180216121A1

公开(公告)日：2018-08-02

申请号：US15747923

申请日：2016-07-28

申请人： KAO CORPORATION

发明人： Toshiharu ARAI ,  Hiroshi KAKESHITA

IPC分类号： C12N15/80 ,  C12N1/14 ,  C12R1/885 ,  C12P1/02 ,  C13K1/02

CPC分类号： C12N15/80 ,  C07K14/37 ,  C12N1/14 ,  C12N9/2437 ,  C12N9/248 ,  C12P1/02 ,  C12P19/02 ,  C12P19/14 ,  C12P21/02 ,  C12R1/885 ,  C13K1/02

摘要： A filamentous fungus mutant strain in which enzyme production inhibition caused by glucose is suppressed is constructed, and a method of producing a polysaccharide-degrading enzyme, a method of producing a saccharide from biomass, and a method of saccharifying biomass, each using the filamentous fungus, are provided. The filamentous fungus mutant strain in which Sre1 expression is reduced compared to a parent strain or is lost.

公开(公告)号：US10036000B2

公开(公告)日：2018-07-31

申请号：US15313568

申请日：2015-05-19

申请人： GENOFOCUS CO., LTD.

发明人： Jae Youl Choi ,  Jae Gu Pan ,  Seung Hwan Park ,  Eui Joong Kim

IPC分类号： C12N9/38 ,  C12N9/10 ,  C12P19/18 ,  C12P21/06 ,  C12P19/34 ,  C12N1/20 ,  C12N1/00 ,  C07H21/04 ,  C13K5/00 ,  C07K1/00 ,  C12P19/04 ,  C12P21/02

CPC分类号： C12N9/2471 ,  C12N15/09 ,  C12P19/04 ,  C12P21/02 ,  C12Y302/01023

摘要： The present invention relates to a novel beta-galactosidase, and more particularly to a novel beta-galactosidase derived from Bacillus circulans, a gene encoding the beta-galactosidase, a recombinant vector and a recombinant microorganism, which contain the gene, a method for producing a beta-galactosidase using the recombinant microorganism, and a method for producing galactooligosaccharide using the beta-galactosidase. The use of the novel beta-galactosidase according to the present invention makes it possible to efficiently produce a large amount of galactooligosaccharide.

公开(公告)号：US10035886B2

公开(公告)日：2018-07-31

申请号：US15285256

申请日：2016-10-04

申请人： Bolt Threads, Inc.

发明人： Daniel M. Widmaier ,  David N. Breslauer ,  Joshua Kittleson ,  Brendan Turner ,  Lindsay Wray

IPC分类号： C08H1/00 ,  C07K14/435 ,  B29C47/00 ,  B29C47/88 ,  D01F4/02 ,  D01F6/68 ,  D01D1/02 ,  D01D5/00 ,  D01F11/02 ,  B29L31/00

CPC分类号： C08H1/00 ,  B29C48/022 ,  B29C48/919 ,  B29K2089/00 ,  B29K2995/0077 ,  B29L2031/731 ,  C07K14/43518 ,  C07K14/43586 ,  C07K2319/02 ,  C07K2319/43 ,  C12P21/00 ,  C12P21/02 ,  D01D1/02 ,  D01D5/00 ,  D01F4/00 ,  D01F4/02 ,  D01F6/68 ,  D01F11/02

摘要： The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

公开(公告)号：US20180208936A1

公开(公告)日：2018-07-26

申请号：US15745030

申请日：2016-07-15

申请人： HONDA MOTOR CO., LTD.

发明人： Satoru SHINKAWA ,  Shigenobu MITSUZAWA ,  Maiko TANAKA ,  Masayuki MACHIDA ,  Hiroko HAGIWARA ,  Hideaki KOIKE ,  Mahoko KUNINAGA

IPC分类号： C12N15/82 ,  C12N15/62 ,  C12P21/02 ,  C12N15/80 ,  C07K14/38

CPC分类号： C12N15/8226 ,  C07K14/38 ,  C12N15/62 ,  C12N15/63 ,  C12N15/80 ,  C12P21/02

摘要： To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequence 1 for protein expression includes: a gene 3 encoding protein 2; a promoter 4 of the gene 3, the promoter being linked upstream of the gene 3; and a cis element 5 whose activity is improved by an artificial transcription factor 6, the cis element being linked further upstream of the promoter 4. The cis element 5 is represented by SEQ ID NO: 1.

公开(公告)号：US10017728B2

公开(公告)日：2018-07-10

申请号：US14786972

申请日：2014-04-24

申请人： NORTHWESTERN UNIVERSITY

发明人： Michael Christopher Jewett ,  Brian Robert Fritz ,  Laura Elyse Timmerman ,  Yi Liu

IPC分类号： C12P19/34 ,  C12P21/02 ,  C12N1/20 ,  C12P21/00

CPC分类号： C12N1/20 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02

摘要： A platform for preparing a sequence defined biopolymer in vitro is disclosed. The platform includes a ribosome-depleted cellular extract ribosomal RNAs prepared by in vitro transcription and purified ribosomal proteins depleted of ribosomal RNAs. A method of synthesizing and assembling ribosomes in vitro for use in the platform is provided, as well as a method for preparing a sequence defined biopolymer in vitro using assembling ribosomes and the platform.

公开(公告)号：US20180187228A1

公开(公告)日：2018-07-05

申请号：US15700467

申请日：2017-09-11

申请人： RINA-NETZWERK RNA TECHNOLOGIEN GMBH

发明人： Michael Gerrits ,  Jan Strey ,  Wolfgang Stiege

IPC分类号： C12P21/00 ,  C12N15/70 ,  C12N1/20 ,  C12P21/02 ,  C12N15/67 ,  C12N1/06

CPC分类号： C12P21/00 ,  C12N1/06 ,  C12N1/20 ,  C12N15/67 ,  C12N15/70 ,  C12P21/02

摘要： The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof.