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公开(公告)号：US20190300924A1
公开(公告)日：2019-10-03
申请号：US16413883
申请日：2019-05-16
发明人： Jan Strey , Helmut Merk , Wolfgang Stiege
摘要： The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).
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公开(公告)号：US20210071220A1
公开(公告)日：2021-03-11
申请号：US16839148
申请日：2020-04-03
发明人： Jan Strey , Helmut Merk , Wolfgang Stiege
摘要： The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).
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公开(公告)号：US20180187228A1
公开(公告)日：2018-07-05
申请号：US15700467
申请日：2017-09-11
发明人： Michael Gerrits , Jan Strey , Wolfgang Stiege
摘要： The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof.
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公开(公告)号：US20170198327A1
公开(公告)日：2017-07-13
申请号：US15469817
申请日：2017-03-27
发明人： Jan Strey , Helmut Merk , Wolfgang Stiege
摘要： The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).
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5.发明申请METHOD FOR THE PRODUCTION OF A LYSATE USED FOR CELL-FREE PROTEIN BIOSYNTHESES 审中-公开用于生产无细胞蛋白质生物试剂的木质素的方法公开(公告)号：US20150140603A1
公开(公告)日：2015-05-21
申请号：US14222937
申请日：2014-03-24
发明人： Michael Gerrits , Jan Strey , Wolfgang Stiege
IPC分类号： C12P21/00
摘要： The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof
摘要翻译： 本发明涉及一种用于细胞费用蛋白质生物合成的裂解物的制备方法，包括以下步骤：a）生物体中的基因组序列，其编码降低细胞费用蛋白质生物合成产率的必需翻译产物， 由位于合适调节元件下的外源DNA代替，所述外源DNA编码另外含有标记序列的必需翻译产物; b）培养根据步骤a）克隆的生物体; c）裂解步骤b）中得到的培养物的生物体; 和d）通过对标记序列具有选择性的分离过程来消除必需的翻译产物。 还讨论了所述裂解物及其用途
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公开(公告)号：US20190284599A1
公开(公告)日：2019-09-19
申请号：US16165067
申请日：2018-10-19
发明人： Michael Gerrits , Jan Strey , Wolfgang Stiege
摘要： The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof.
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公开(公告)号：US20170152539A1
公开(公告)日：2017-06-01
申请号：US14976509
申请日：2015-12-21
发明人： Michael Gerrits , Jan Strey , Wolfgang Stiege
摘要： The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof.
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公开(公告)号：US20140162313A1
公开(公告)日：2014-06-12
申请号：US14152221
申请日：2014-01-10
发明人： Jan Strey , Helmut Merk , Wolfgang Stiege
IPC分类号： C12P21/00
摘要： The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).
摘要翻译： 本发明涉及无细胞转录/翻译系统中制备型体外蛋白质合成的方法，包括以下步骤：a）在反应容器中制备反应溶液，其包含以下合成物质：转录成分 /定义的蛋白质，氨基酸和代谢组分的翻译装置，其提供能量并且是合成定义的蛋白质所必需的，b）在规定的时间段内在反应容器中进行合成，c） 在确定的时间段内，将反应溶液进行分离步骤，其中产生的低分子代谢产物与溶液分离（并萃取）。
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9.发明申请METHOD FOR THE PRODUCTION OF A LYSATE USED FOR CELL-FREE PROTEIN BIOSYNTHESES 审中-公开用于生产无细胞蛋白质生物试剂的木质素的方法公开(公告)号：US20130115655A1
公开(公告)日：2013-05-09
申请号：US13631146
申请日：2012-09-28
发明人： Michael Gerrits , Jan Strey , Wolfgang Stiege
IPC分类号： C12P21/00
摘要： The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof.
摘要翻译： 本发明涉及一种用于细胞费用蛋白质生物合成的裂解物的制备方法，包括以下步骤：a）生物体中的基因组序列，其编码降低细胞费用蛋白质生物合成产率的必需翻译产物， 由位于合适调节元件下的外源DNA代替，所述外源DNA编码另外含有标记序列的必需翻译产物; b）培养根据步骤a）克隆的生物体; c）裂解步骤b）中获得的培养物的生物体; 和d）通过对标记序列具有选择性的分离过程来消除必需的翻译产物。 还讨论了所述裂解物及其用途。
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