公开(公告)号：US20190300924A1

公开(公告)日：2019-10-03

申请号：US16413883

申请日：2019-05-16

Applicant: Rina-Netzwerk RNA Technologien GmbH

Inventor： Jan Strey ,  Helmut Merk ,  Wolfgang Stiege

IPC: C12P21/00 ,  C07K1/34

Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).

公开(公告)号：US20170198327A1

公开(公告)日：2017-07-13

申请号：US15469817

申请日：2017-03-27

Applicant: Rina-Netzwerk RNA Technologien GmbH

Inventor： Jan Strey ,  Helmut Merk ,  Wolfgang Stiege

IPC: C12P21/00 ,  C07K1/34

CPC classification number: C12P21/00 ,  C07K1/34

Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).

公开(公告)号：US20210071220A1

公开(公告)日：2021-03-11

申请号：US16839148

申请日：2020-04-03

Applicant: Rina-Netzwerk RNA Technologien GmbH

Inventor： Jan Strey ,  Helmut Merk ,  Wolfgang Stiege

IPC: C12P21/00 ,  C07K1/34

Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).

公开(公告)号：US20140162313A1

公开(公告)日：2014-06-12

申请号：US14152221

申请日：2014-01-10

Applicant: Rina-Netzwerk RNA Technologien GmbH

Inventor： Jan Strey ,  Helmut Merk ,  Wolfgang Stiege

IPC: C12P21/00

CPC classification number: C12P21/00 ,  C07K1/34

Abstract: The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted).

Abstract translation: 本发明涉及无细胞转录/翻译系统中制备型体外蛋白质合成的方法，包括以下步骤：a）在反应容器中制备反应溶液，其包含以下合成物质：转录成分 /定义的蛋白质，氨基酸和代谢组分的翻译装置，其提供能量并且是合成定义的蛋白质所必需的，b）在规定的时间段内在反应容器中进行合成，c） 在确定的时间段内，将反应溶液进行分离步骤，其中产生的低分子代谢产物与溶液分离（并萃取）。