公开(公告)号：US11666874B2

公开(公告)日：2023-06-06

申请号：US16052705

申请日：2018-08-02

申请人： Massachusetts Institute of Technology

发明人： David Lai ,  Filippos Touriomousis ,  Andreas Mershin ,  Neil Gershenfeld

IPC分类号： B01F23/41 ,  B01F33/3011 ,  C09D11/102 ,  A61K9/107 ,  B33Y80/00 ,  B01L3/00 ,  B01F33/81 ,  B01F35/00 ,  B01F35/22 ,  B01F101/22

CPC分类号： B01F33/3011 ,  A61K9/107 ,  B01F23/41 ,  B01F33/813 ,  B01F35/2202 ,  B01F35/561 ,  B01L3/502784 ,  B33Y80/00 ,  C09D11/102 ,  B01F23/4143 ,  B01F2101/22 ,  B01L2300/0887

摘要： The invention comprises a novel modular, generalizable meso-micro-nano-fluidic platform apparatus, design and methodology which in exemplary embodiments may be applied in conjunction with a novel external triggering and automation/feedback loop control mechanism deployed via computer to explore the phase space of single or double emulsification for applications including the encapsulation of hydrophilic active pharmacological ingredients (APIs). End use applications include the mass production of particulate encapsulation of hydrophobic or hydrophilic APIs with automatic or user-supervised feedback methodology to control and discover mass production or per-drug customized settings of interest for the manufacture of novel or extant therapeutics. This invention allows for a process to produce monodispersed particles of varying sizes and may be used to rapidly screen for optimal size for maximal bioavailability of API particles either on lab bench for in vitro dissolution or in vivo studies, and patient-specific handhelds for maximal drug inhalation.

公开(公告)号：US12121898B2

公开(公告)日：2024-10-22

申请号：US17148287

申请日：2021-01-13

申请人： President and Fellows of Harvard College

发明人： David A. Weitz ,  Adam R. Abate

IPC分类号： B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/302 ,  B01L3/02

CPC分类号： B01L3/502761 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/3021 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0681 ,  Y10T137/0318 ,  Y10T137/8593

摘要： The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.

公开(公告)号：US12103005B2

公开(公告)日：2024-10-01

申请号：US16995555

申请日：2020-08-17

申请人： Bio-Rad Laboratories, Inc.

发明人： Kevin D. Ness ,  Christopher F. Kelly ,  Donald A. Masquelier

IPC分类号： B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01L3/02 ,  B01L7/00 ,  C12Q1/6806 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC分类号： B01L3/502784 ,  B01F23/41 ,  B01F33/3011 ,  B01L3/502715 ,  B01L3/502723 ,  C12Q1/6806 ,  G01N35/085 ,  B01L3/0275 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2400/0487 ,  G01N2035/00148 ,  G01N2035/1034 ,  G01N35/1065 ,  Y10T29/494

摘要： A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US20240280569A1

公开(公告)日：2024-08-22

申请号：US18653506

申请日：2024-05-02

申请人： Bio-Rad Laboratories, Inc.

发明人： Michael L. Samuels ,  Darren Roy Link

IPC分类号： G01N33/543 ,  B01F33/3011 ,  B01F33/302 ,  C12Q1/6874 ,  C40B30/04 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532

CPC分类号： G01N33/54366 ,  C12Q1/6874 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  B01F33/3011 ,  B01F33/3021 ,  C40B30/04

摘要： The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US11898193B2

公开(公告)日：2024-02-13

申请号：US16565773

申请日：2019-09-10

申请人： Bio-Rad Laboratories, Inc.

发明人： Benjamin J. Miller ,  Qun Zhong ,  Darren Roy Link

IPC分类号： C12Q1/6806 ,  B05B1/08 ,  B01L3/00 ,  B05B1/26 ,  B05B7/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/221 ,  B05B1/02 ,  B01L7/00

CPC分类号： C12Q1/6806 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/2213 ,  B01L3/502761 ,  B01L3/502784 ,  B05B1/02 ,  B05B1/08 ,  B05B1/26 ,  B05B7/0012 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/0647 ,  B01L2200/141 ,  B01L2200/143 ,  B01L2200/148 ,  B01L2300/0867 ,  B01L2300/14 ,  B01L2400/0487 ,  Y10T436/2575

摘要： The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.

公开(公告)号：US11873173B2

公开(公告)日：2024-01-16

申请号：US18136126

申请日：2023-04-18

申请人： CYTONOME/ST, LLC

发明人： John R. Gilbert ,  Manish Deshpande ,  Bernard Bunner

IPC分类号： B65G51/08 ,  B01L3/00 ,  G01N15/14 ,  B07C5/00 ,  B07C5/34 ,  B01F33/301 ,  B65G51/00 ,  B01F33/3011

CPC分类号： B65G51/08 ,  B01F33/3017 ,  B01L3/502776 ,  B07C5/00 ,  B07C5/34 ,  G01N15/1404 ,  G01N15/1484 ,  B01F33/3011 ,  B01J2219/0086 ,  B01J2219/00822 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00891 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0487 ,  B65G51/00 ,  G01N2015/1409

摘要： A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

公开(公告)号：US20230408505A1

公开(公告)日：2023-12-21

申请号：US18354144

申请日：2023-07-18

申请人： Bio-Rad Laboratories, Inc.

发明人： Michael L. Samuels ,  Darren Roy Link

IPC分类号： G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874

CPC分类号： G01N33/54366 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F33/3011

摘要： The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20230393069A1

公开(公告)日：2023-12-07

申请号：US18448631

申请日：2023-08-11

申请人： Bio-Rad Laboratories, inc.

发明人： Darren Roy Link ,  Michael L. Samuels

IPC分类号： G01N21/64 ,  B01J19/00 ,  B01L3/00 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58

CPC分类号： G01N21/6445 ,  B01J19/0046 ,  B01L3/502761 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/582 ,  B01L7/52

摘要： The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US11819816B2

公开(公告)日：2023-11-21

申请号：US16806961

申请日：2020-03-02

申请人： INVENTAGE LAB, INC.

发明人： Ju Hee Kim

IPC分类号： B01J13/06 ,  A61K9/00 ,  B01L3/00 ,  B01J19/00 ,  A61K9/16 ,  B01F33/302 ,  B01F33/30 ,  B01F33/3011 ,  A61K9/14 ,  A61K31/365 ,  A61K31/58 ,  C08J5/00 ,  B01J2/06 ,  B01J2/02

CPC分类号： B01J13/06 ,  A61K9/0019 ,  A61K9/146 ,  A61K9/1647 ,  A61K9/1682 ,  A61K31/365 ,  A61K31/58 ,  B01F33/30 ,  B01F33/302 ,  B01F33/3011 ,  B01J19/0093 ,  B01L3/502707 ,  C08J5/00 ,  B01J2/02 ,  B01J2/06 ,  B01J2219/00889 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/161 ,  C08J2367/04

摘要： Provided is an apparatus for a mass production of microspheres and a multichannel forming device incorporatable therein. The apparatus includes a multi-channel microsphere forming unit, a first source material reservoir containing the first source material and in fluid communication with the plurality of first microchannels, a second source material reservoir containing the second source material and in fluid communication with the plurality of second microchannels, a flow control unit configured to supply a first gas to the first source material reservoir at a first source material flow rate and to supply a second gas to a second source material reservoir at a second source material flow rate and a product reservoir for accommodating the microspheres formed from the multi-channel forming unit.

公开(公告)号：US20230302420A1

公开(公告)日：2023-09-28

申请号：US17978304

申请日：2022-11-01

申请人： President and Fellows of Harvard College

发明人： David A. Weitz ,  Mark Romanowsky ,  Adam R. Abate

IPC分类号： B01F33/3011 ,  B01L3/00 ,  B01F23/41 ,  B01J19/00

CPC分类号： B01F33/3011 ,  B01L3/502784 ,  B01F23/41 ,  B01F23/4105 ,  B01J19/0093 ,  Y10T137/8593 ,  B01J2219/00015 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/0097 ,  B01J2219/00975 ,  B01F2101/23

摘要： Parallel uses of microfluidic methods and devices for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid are described. In some aspects, the present invention relates generally to flow-focusing-type technology, and also to microfluidics, and more particularly parallel use of microfluidic systems arranged to control a dispersed phase within a dispersant, and the size, and size distribution, of a dispersed phase in a multi-phase fluid system, and systems for delivery of fluid components to multiple such devices.