公开(公告)号：US10272432B2

公开(公告)日：2019-04-30

申请号：US16160783

申请日：2018-10-15

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Christopher F. Kelly ,  Donald A. Masquelier

IPC: B01L3/02 ,  B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  C12Q1/6806 ,  G01N35/08 ,  G01N35/10 ,  G01N35/00 ,  B01L7/00

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US12103005B2

公开(公告)日：2024-10-01

申请号：US16995555

申请日：2020-08-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Christopher F. Kelly ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01L3/02 ,  B01L7/00 ,  C12Q1/6806 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC classification number: B01L3/502784 ,  B01F23/41 ,  B01F33/3011 ,  B01L3/502715 ,  B01L3/502723 ,  C12Q1/6806 ,  G01N35/085 ,  B01L3/0275 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2400/0487 ,  G01N2035/00148 ,  G01N2035/1034 ,  G01N35/1065 ,  Y10T29/494

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US10744506B2

公开(公告)日：2020-08-18

申请号：US16397774

申请日：2019-04-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Christopher F. Kelly ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08 ,  G01N35/08 ,  C12Q1/6806 ,  B01L3/02 ,  B01L7/00 ,  G01N35/10 ,  G01N35/00

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US10099219B2

公开(公告)日：2018-10-16

申请号：US15357840

申请日：2016-11-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Christopher F. Kelly ,  Donald A. Masquelier

IPC: B01L3/00 ,  G01N35/08 ,  C12Q1/6806 ,  B01L3/02 ,  B01L7/00 ,  G01N35/10 ,  G01N35/00

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US20170065979A1

公开(公告)日：2017-03-09

申请号：US15357840

申请日：2016-11-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Christopher F. Kelly ,  Donald A. Masquelier

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01L3/0275 ,  B01L3/502715 ,  B01L3/502723 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2400/0487 ,  C12Q1/6806 ,  G01N35/085 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/1034 ,  Y10T29/494

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

Abstract translation: 一种用于产生适合于基于液滴的测定的液滴的方法和装置的系统。 所公开的系统可以包括通过将含水样品的流体与背景乳液流体（例如油）并入而形成含有样品的液滴的乳液，其被配置成通过将含水样品的流体与背景乳液流体例如油混合而构成用于形成含样品的液滴的单件或多件式液滴产生组件 在背景液中。 在一些情况下，所公开的系统可以包括被配置成将含样品的液滴输送到出口区域的通道或其它合适的机制，从而可以执行随后的测定步骤。