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公开(公告)号：US20190071704A1
公开(公告)日：2019-03-07
申请号：US16041734
申请日：2018-07-20
Applicant: GENERAL ELECTRIC COMPANY
Inventor： John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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公开(公告)号：US20180345278A1
公开(公告)日：2018-12-06
申请号：US15611586
申请日：2017-06-01
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Christine Lynne Surrette , Erik Leeming Kvam , Steven Yuehin Go , Feng Chen , John Richard Nelson , Craig Patrick Galligan , Radislav Alexandrovich Potyrailo , Gregory Andrew Grossmann
IPC: B01L3/00 , C12Q1/02 , G01N21/64 , G01N21/76 , G01N33/554
CPC classification number: C12Q1/02 , C12M41/00 , C12M41/46 , C12Q1/18 , G01N21/6408 , G01N21/6452 , G01N33/4836
Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.
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公开(公告)号：US09938568B2
公开(公告)日：2018-04-10
申请号：US14933275
申请日：2015-11-05
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ryan Charles Heller , Erik Leeming Kvam , John Richard Nelson
CPC classification number: C12Q1/6844 , C12Q1/6869 , C12Q2521/501 , C12Q2525/307 , C12Q2527/137 , C12Q2531/125
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.
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公开(公告)号：US20130289257A1
公开(公告)日：2013-10-31
申请号：US13721948
申请日：2012-12-20
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Brian Christopher Bales , Erik Leeming Kvam , Jason Louis Davis
IPC: B01J20/04
CPC classification number: B01J20/045 , B01J20/24 , C12N15/1006 , C12N15/1017 , G01N1/40
Abstract: The present disclosure generally relates to dry solid matrices for the extraction, stabilization, and storage of nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.
Abstract translation: 本公开内容通常涉及用于在环境条件下长时间提取，稳定和储存核酸，特别是RNA的干固体基质。 还描述了收集和回收存储在干燥固体基质中的核酸的方法。
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公开(公告)号：US10787695B2
公开(公告)日：2020-09-29
申请号：US15611586
申请日：2017-06-01
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Christine Lynne Surrette , Erik Leeming Kvam , Steven Yuehin Go , Feng Chen , John Richard Nelson , Craig Patrick Galligan , Radislav Alexandrovich Potyrailo , Gregory Andrew Grossmann
IPC: C12Q1/02 , C12M1/34 , G01N21/64 , C12Q1/18 , G01N33/483
Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.
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Patent Agency Ranking
公开(公告)号：US10518196B2
公开(公告)日：2019-12-31
申请号：US15209411
申请日：2016-07-13
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Christopher Michael Puleo , Craig Patrick Galligan , Gregory Andrew Grossman , Erik Leeming Kvam , Robert Scott Duthie , Kenneth Wayne Rigby , Paul Michael Smigelski, Jr. , Victoria Eugenia Cotero , Jason William Castle , John Donald Burczak , James Edward Rothman
IPC: B01D21/00 , B01D21/24 , G01N33/49 , B01L3/00 , G01N1/40 , C12M1/12 , C12M1/26 , B01D17/02 , B01D21/02 , C12M1/00 , C02F1/48 , C02F1/44 , C02F101/32
Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.
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公开(公告)号：US20180354987A1
公开(公告)日：2018-12-13
申请号：US15619148
申请日：2017-06-09
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , Bruce Allan Hay , Ernest William Kovacs
Abstract: A method for purification of a target in a solution includes providing a target in a solution, the solution comprising one or more contaminants, providing a polymer conjugate in the solution, wherein the polymer conjugate is configured to specifically bind the target, incubating the solution at a first temperature to facilitate binding of the polymer conjugate to the target, providing an environmentally-responsive block copolymer in the solution, wherein the environmentally-responsive block copolymer comprises one or more of an ethylene oxide (EO), a propylene oxide (PO), or an EO/PO block copolymer, and heating the solution comprising the environmentally-responsive block copolymer and the polymer conjugate bound to the target at a second temperature to initiate a liquid-liquid phase separation, wherein the liquid-liquid phase separation produces an aqueous phase and a liquid polymer phase, and wherein the liquid polymer phase comprises the polymer conjugate bound to the target.
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公开(公告)号：US20180273914A1
公开(公告)日：2018-09-27
申请号：US16005633
申请日：2018-06-11
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Erik Leeming Kvam , Robert Scott Duthie , John Richard Nelson
IPC: C12N7/00 , C12Q1/70 , C12Q1/6806 , B01L3/00 , C12N15/10
CPC classification number: C12N7/00 , B01L3/508 , B01L2200/082 , C12N15/1017 , C12N2760/14111 , C12N2760/14151 , C12N2760/14163 , C12Q1/6806 , C12Q1/70 , C12Q1/701 , C12Q2523/113 , C12Q2523/308 , C12Q2527/125
Abstract: The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.
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公开(公告)号：US20170211059A1
公开(公告)日：2017-07-27
申请号：US15463943
申请日：2017-03-20
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , John Richard Nelson , Gregory Andrew GROSSMANN , Ryan Charles Heller , Erin Jean Finehout , Christopher Michael Puleo , William Patrick Waters
CPC classification number: C12N15/1017 , B01L3/5023 , B01L7/52 , B01L2300/0681 , B01L2300/0825 , C12Q1/68 , C12Q1/6844 , C12Q1/6806 , C12Q2521/501 , C12Q2531/125
Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.
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公开(公告)号：US20170145387A1
公开(公告)日：2017-05-25
申请号：US14945483
申请日：2015-11-19
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Erik Leeming Kvam , Robert Scott Duthie , John Richard Nelson
CPC classification number: C12N7/00 , B01L3/508 , B01L2200/082 , C12N15/1017 , C12N2760/14111 , C12N2760/14151 , C12N2760/14163 , C12Q1/6806 , C12Q1/70 , C12Q1/701 , C12Q2523/113 , C12Q2523/308 , C12Q2527/125
Abstract: The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.
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