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公开(公告)号：US20180223349A1
公开(公告)日：2018-08-09
申请号：US15949061
申请日：2018-04-09
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ryan Charles Heller , Erik Leeming Kvam , John Richard Nelson
IPC: C12Q1/6844 , C12Q1/6869
CPC classification number: C12Q1/6844 , C12Q1/6869 , C12Q2521/501 , C12Q2525/179 , C12Q2531/125
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.
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公开(公告)号：US20160281153A1
公开(公告)日：2016-09-29
申请号：US15177149
申请日：2016-06-08
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson
CPC classification number: C12Q1/6853 , C12P19/34 , C12Q1/6865 , C12Q2521/301 , C12Q2523/31 , C12Q2525/179 , C12Q2531/119 , C12Q2531/125 , C12Q2531/143
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal DNA amplification methods that employ a strand displacing DNA polymerase and polyamine-oligonucleotide conjugate primer are also provided.
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公开(公告)号：US09410172B2
公开(公告)日：2016-08-09
申请号：US14027947
申请日：2013-09-16
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson
CPC classification number: C12Q1/6853 , C12P19/34 , C12Q1/6865 , C12Q2521/301 , C12Q2523/31 , C12Q2525/179 , C12Q2531/119 , C12Q2531/125 , C12Q2531/143
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal DNA amplification methods that employ a strand displacing DNA polymerase and polyamine-oligonucleotide conjugate primer are also provided.
Abstract translation: 本文提供了使用寡聚寡核苷酸共轭引物扩增靶核酸以产生扩增子的等温核酸扩增的方法和试剂盒。 还提供了使用链置换DNA聚合酶和多胺 - 寡核苷酸共轭引物的等温DNA扩增方法。
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公开(公告)号：US20150031086A1
公开(公告)日：2015-01-29
申请号：US13952040
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson , Erik Leeming Kvam
IPC: C12P19/34
CPC classification number: C12P19/34 , C12Q1/6869 , C12Q2521/501 , C12Q2525/307 , C12Q2527/137
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of circulating nucleic acids extracted from blood is provided. Kits for performing the disclosed methods are also provided.
Abstract translation: 本文提供了在单个反应容器中从线性DNA产生和扩增单链DNA环的方法，而没有任何介入的纯化步骤。 通过不依赖模板的单链DNA连接产生单链DNA环。 提供了从血液中提取的循环核酸的全基因组扩增。 还提供了用于执行所公开的方法的套件。
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公开(公告)号：US09920359B2
公开(公告)日：2018-03-20
申请号：US15177149
申请日：2016-06-08
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson
CPC classification number: C12Q1/6853 , C12P19/34 , C12Q1/6865 , C12Q2521/301 , C12Q2523/31 , C12Q2525/179 , C12Q2531/119 , C12Q2531/125 , C12Q2531/143
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use an oligocation-oligonucleotide conjugate primer for amplifying a target nucleic acid to generate amplicons. Isothermal DNA amplification methods that employ a strand displacing DNA polymerase and polyamine-oligonucleotide conjugate primer are also provided.
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Patent Agency Ranking
6.发明申请METHOD AND DEVICE FOR COLLECTION AND AMPLIFICATION OF CIRCULATING NUCLEIC ACIDS 有权用于收集和放大循环核酸的方法和装置公开(公告)号：US20150031035A1
公开(公告)日：2015-01-29
申请号：US13952173
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , John Richard Nelson , Gregory Andrew Grossmann , Ryan Charles Heller , Erin Jean Finehout , Christopher Michael Puleo , William Patrick Waters
IPC: C12Q1/68
CPC classification number: C12N15/1017 , B01L7/52 , B01L2300/0681 , C12Q1/68 , C12Q1/6844 , C12Q1/6806 , C12Q2521/501 , C12Q2531/125
Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.
Abstract translation: 本文提供了从生物样品的非细胞部分收集和扩增循环核酸的方法。 从非细胞级分提取循环核酸，并进行环化以产生单链核酸环，然后通过使用随机引物的滚动循环扩增产生扩增文库，然后扩增其。 还提供了用于从生物样品中收集非细胞级分的装置。 该装置包括与过滤膜直接接触的过滤膜和干燥固体基质。
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公开(公告)号：US10597691B2
公开(公告)日：2020-03-24
申请号：US15408509
申请日：2017-01-18
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson , Paresh Lakhubhai Patel , Alison Myfanwy Wakefield , Stephen James Capper
IPC: C12P19/34 , C12Q1/6844
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.
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公开(公告)号：US20170211059A1
公开(公告)日：2017-07-27
申请号：US15463943
申请日：2017-03-20
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , John Richard Nelson , Gregory Andrew GROSSMANN , Ryan Charles Heller , Erin Jean Finehout , Christopher Michael Puleo , William Patrick Waters
CPC classification number: C12N15/1017 , B01L3/5023 , B01L7/52 , B01L2300/0681 , B01L2300/0825 , C12Q1/68 , C12Q1/6844 , C12Q1/6806 , C12Q2521/501 , C12Q2531/125
Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.
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公开(公告)号：US09644232B2
公开(公告)日：2017-05-09
申请号：US13952173
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , John Richard Nelson , Gregory Andrew Grossmann , Ryan Charles Heller , Erin Jean Finehout , Christopher Michael Puleo , William Patrick Waters
IPC: C12Q1/68
CPC classification number: C12N15/1017 , B01L7/52 , B01L2300/0681 , C12Q1/68 , C12Q1/6844 , C12Q1/6806 , C12Q2521/501 , C12Q2531/125
Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.
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公开(公告)号：US20170121747A1
公开(公告)日：2017-05-04
申请号：US15408509
申请日：2017-01-18
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson , Paresh Lakhubhai Patel , Alison Myfanwy Wakefield , Stephen James Capper
IPC: C12P19/34
CPC classification number: C12P19/34 , C12Q1/6844 , C12Q2527/107 , C12Q2527/125 , C12Q2527/137 , C12Q2531/119
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.
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